Associations of −308G/APolymorphism ofTumor Necrosis Factor(TNF)–αGene and Serum TNF-αLevels with Measures of Obesity, Intra-Abdominal and Subcutaneous Abdominal Fat, Subclinical Inflammation and Insulin Resistance in Asian Indians in North India

Abstract

Objectives:Obesity is associated with high levels proinflammatory cytokines like tumour necrosis factor alpha (TNF-α), which may play an important role in the genesis of insulin resistance. We evaluated the relationship of −308G/Apolymorphism ofTNF-αgene with obesity and insulin resistance in Asian Indians in north India.Methods:This cross-sectional study included 151 apparently healthy individuals (79 males, 72 females) 18–50 yrs of age from New Delhi, India. Body composition by dual-energy x-ray absorptiometry (DEXA) and abdominal fat by magnetic resonance imaging (MRI) were measured. Biochemical measurements included OGTT, lipids, fasting insulin, hs-CRP and TNF-αlevels. We analysed −308G/Apolymorphism ofTNF-αgene and studied its association with obesity and biochemical parameters.Results:At comparable BMI, abdominal obesity was more prevalent in females (50%) as compared to males (20%). The wild genotype (GG) was present in 78.8%, GA in 17.9%, andAAin 3.3% subjects. Measures of body composition, abdominal fat distribution, lipids, insulin, hs-CRP and TNF-αlevels were not influenced by the presence of −308G/Apolymorphism. Serum TNF-αlevels correlated significantly with fasting insulin in both genders.Conclusion:TNF-αlevels correlate with fasting insulin but not with indicators of body composition in Asian Indians. The −308G/Apolymorphism ofTNF-αgene is not associated with differences in the serum levels of TNF-αin Asian Indians.

TNF‐α and IL‐10 polymorphisms increase the risk to hepatocellular carcinoma in HCV infected individuals

“…Some studies have demonstrated that the TNF‐α G allele is associated with low TNF‐α production in vitro and in vivo [Kroeger et al, , ; Wilson et al, ; Karimi et al, ; Baghel et al, ]. However, most studies failed to confirm this correlation [Brinkman et al, ps11996; Skoog et al, ; Bayley et al, ; Kaijzel et al, ; Knight et al, ; Cuchacovich et al, ; Marotte et al, ; O'Dwyer et al, ; Vikram et al, ]. Additionally, Mekinian et al [], through a functional study with a meta‐analysis including 21 studies, found no significant association between TNF‐α production and the TNF‐α ‐308 SNP (rs1800629) [Mekinian et al, ].…”

Impact of host gene polymorphisms on susceptibility to chronic hepatitis B virus infection

“…Similarly, an Iranian study did not report any significant association between TNF-α polymorphisms and HBV pathogenesis (Somi et al, 2006). On the contrary, there was not significant relationship between TNF-α polymorphisms and rheumatoid arthritis (Chen et al, 2007;Cuenca et al, 2003;Vikram et al, 2011) and chronic periodontitis Heidari et al, 2014a;Solhjoo et al, 2014) as inflammatory diseases. Although, it seems that TNF-α polymorphisms can block the HBV gene expression via alteration the expression of TNF-α but conflicting data will need further evaluation to show the real effect of TNF-α polymorphisms in HBV infection.…”

TNF-αG-308A polymorphism is associated with insulin resistance: a meta-analysis

“…The remained articles were further evaluated for appropriateness and 32 articles were excluded (Figure 1). Finally, 15 articles met the inclusion criteria (Fernandez-Real et al, 1997;Walston et al, 1999;Hayakawa et al, 2000;Hoffstedt et al, 2000;Ishii et al, 2000;Rasmussen et al, 2000;Brand et al, 2001;Romeo et al, 2001;Dalziel et al, 2002;Um et al, 2003;Wybranska et al, 2003;De Luis et al, 2011;Vikram et al, 2011;Hedayati et al, 2012;Wingeyer et al, 2012). One article (Ishii et al, 2000) provided separate data on young (aged 21 to 29) and older (aged 45 to 65) subjects, and one article (Hoffstedt et al, 2000) on male and female subjects.…”

“…Five studies involved lean subjects with normal weight (BMI < 25 kg/m 2 ), 3 involved obese subjects (BMI ≥ 30 kg/m 2 ), and 9 involved both obese and lean subjects. The genotype distributions in two studies were deviated from HWE (Wybranska et al, 2003;Vikram et al, 2011). The studies were published between 1997 and 2012.…”