Associations between the use of antimicrobial agents for growth promotion and the occurrence of antimicrobial-resistant Escherichia coli and enterococci in the feces of livestock and livestock farmers in Japan

Katsunuma, Yu; Hanazumi, Michito; Fujisaki, Hirokazu; Minato, Hajime; Hashimoto, Yasushi; Yonemochi, Chisato

doi:10.2323/jgam.53.273

Cited by 30 publications

(19 citation statements)

References 19 publications

(19 reference statements)

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“…Six fecal samples from animals and one fecal sample from each of two livestock farmers were collected for each farm. destomycin-A (DM), sulfaquinoxaline (SQ), bacitracin (BC), and tylosin (TS) have been described in the previous paper (Katsunuma et al, 2007). A total of 2,929 E. coli and E. faecalis-faecium isolates from animal and livestock farmer fecal samples were collected.…”

Section: Methodsmentioning

confidence: 99%

Comparison of pulsed-field gel electrophoresis patterns of antimicrobial-resistant Escherichia coli and enterococci isolates from the feces of livestock and livestock farmers in Japan

Katsunuma¹,

Hanazumi²,

Fujisaki³

et al. 2008

J. Gen. Appl. Microbiol.

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Section: Methodsmentioning

confidence: 99%

Comparison of pulsed-field gel electrophoresis patterns of antimicrobial-resistant Escherichia coli and enterococci isolates from the feces of livestock and livestock farmers in Japan

Katsunuma¹,

Hanazumi²,

Fujisaki³

et al. 2008

J. Gen. Appl. Microbiol.

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“…24,2011 FOOD ANIMALS AND ANTIMICROBIALS: HUMAN HEALTH IMPACTS 719 but possess the potential to become serious human pathogens. The prospective farm study by Levy in 1975 (111) and studies of others in the following decades clearly demonstrated the selective nature of low-dose, nontherapeutic AGPs on both the pathogenic and commensal flora of food animals such as poultry, swine, and cattle (8,16,18,90,98,146,149). Likewise, in the past decade, studies have demonstrated the selective nature of mass treatment with antimicrobials in aquaculture (36,62,84).…”

Section: Impacts Of Nontherapeutic Usementioning

confidence: 99%

“…The observation extended to the farm family as well and showed an increased frequency of tetracyclineresistant and multidrug-resistant E. coli after several months of use of AGP-laden feed. Studies such as this (which examined a variety of antibiotic classes and assorted pathogens) have consistently shown a higher prevalence of resistant gut bacteria among farm workers than in the general public or among workers on farms not using antibiotics (16,90,98,149). While gentamicin is not approved for growth promotion in the United States, it remains the most commonly used antibiotic in broiler production, being employed for prevention of early poultry mortality (115).…”

Section: Resistance Acquisition Through Direct Contact With Animalsmentioning

confidence: 99%

Food Animals and Antimicrobials: Impacts on Human Health

2011

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SUMMARY Antimicrobials are valuable therapeutics whose efficacy is seriously compromised by the emergence and spread of antimicrobial resistance. The provision of antibiotics to food animals encompasses a wide variety of nontherapeutic purposes that include growth promotion. The concern over resistance emergence and spread to people by nontherapeutic use of antimicrobials has led to conflicted practices and opinions. Considerable evidence supported the removal of nontherapeutic antimicrobials (NTAs) in Europe, based on the “precautionary principle.” Still, concrete scientific evidence of the favorable versus unfavorable consequences of NTAs is not clear to all stakeholders. Substantial data show elevated antibiotic resistance in bacteria associated with animals fed NTAs and their food products. This resistance spreads to other animals and humans—directly by contact and indirectly via the food chain, water, air, and manured and sludge-fertilized soils. Modern genetic techniques are making advances in deciphering the ecological impact of NTAs, but modeling efforts are thwarted by deficits in key knowledge of microbial and antibiotic loads at each stage of the transmission chain. Still, the substantial and expanding volume of evidence reporting animal-to-human spread of resistant bacteria, including that arising from use of NTAs, supports eliminating NTA use in order to reduce the growing environmental load of resistance genes.

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“…Studies suggested that antibiotic-resistant bacteria and resistance genes can enter into the community settings via environmental spread from farms, slaughterhouses as well as occupational workers and their families (Marshall & Levy, 2011). Katsunuma et al reported a significantly high prevalence of AMR in commensal bacteria among poultry, pigs and cattle farm workers around Japan, suggesting occupational exposure risk factor for spreading AMR (Katsunuma et al, 2007). In another study, Aubry-Damon et al…”

Section: Productionmentioning

confidence: 99%

Antimicrobial Resistance of Commensal Escherichia coli Isolated from Food Animals in Qatar

Alhababi

Eltai

Nasrallah

et al. 2020

Microbial Drug Resistance

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Background: The dissemination of antimicrobial resistance (AMR) bacteria is associated with the inappropriate use of antibiotics in both humans and animals. In particular, the use of antibiotics for prophylactic and growth promotion purposes in foodproducing animals, in addition to animal-group medication for prolonged times, have rendered many of the antibiotics ineffective, returning us to the old times when a simple infection could be deadly. Objectives: This study aims at evaluating the incidence of AMR in Qatar under the one health approach; i.e., human-animal-environmental interface.Specifically, we aim to characterize the phenotypic and genotypic AMR pattern of 18 clinically relevant antibiotics in major food-producing animals. Ultimately, we aim to compare our findings to the data released by the monitoring programs in Hamad Medical Corporation (HMC) along with previously published reports from Qatar. Methods: Animal fecal samples from camels, cattle, and pigeons (100 samples from each species) were collected from slaughterhouses and farms distributed in different locations in Qatar. One gram of fecal sample was vigorously homogenized with 3 ml sterile Phosphate Buffered Saline (PBS), the suspension was streaked directly onto chromogenic Tryptone Bile Xglucuronide agar for E. coli detection. Single typical E. coli colonies (blue-green) were randomly selected and streaked onto MacConkey agar plates and then recovered on iv nutrient agar plates for lactose fermentation testing by indole spot. The antibiotic susceptibility test was conducted using a Kirby-Bauer disk diffusion assay on Mueller-Hinton agar plates. Results: A total recovery rate of 88.7% (n=266) achieved from all samples. Overall, E. coli isolates had resistance to ten antibiotics in pigeon group, eight antibiotics in cattle group and only five antibiotics in camel group. Resistance to at least one antibiotic was recorded in 63 E. coli isolates (70.7%) from pigeons, 32 isolates (37.2%) from cattle and only in 19 isolates (20.8%) from camels. Multi-drug resistant (MDR) was highest in isolates from pigeons reaching 50% (n=44), followed by isolates from cattle (7%) and camels (2.2%). Highest resistance rate was observed against tetracycline, reaching a frequency of 64%, 27.9% and 15% of isolates from pigeons, cattle and camels, respectively (p <0.0001). Moreover, trimethoprim/sulfamethoxazole (an antibiotic used to treat a variety of bacterial infections) resistance was present in 22.2% (n=59) of all E. coli isolates, with the highest prevalence in pigeons (49.4%), followed by 11.6% in cattle and 5% in camels. Interestingly, one E. coli isolate from pigeon showed resistance to colistin, a drug of last resort in human medicine against gram-negative bacterial infection.Conclusions: We previously reported high multi-drug resistance of commensal E. coli in chickens, with significant resistance to colistin. We observed lower AMR profile in ruminants, presumably due to the different antibiotics used in the two animal industries.

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Associations between the use of antimicrobial agents for growth promotion and the occurrence of antimicrobial-resistant Escherichia coli and enterococci in the feces of livestock and livestock farmers in Japan

Cited by 30 publications

References 19 publications

Comparison of pulsed-field gel electrophoresis patterns of antimicrobial-resistant Escherichia coli and enterococci isolates from the feces of livestock and livestock farmers in Japan

Comparison of pulsed-field gel electrophoresis patterns of antimicrobial-resistant Escherichia coli and enterococci isolates from the feces of livestock and livestock farmers in Japan

Food Animals and Antimicrobials: Impacts on Human Health

Antimicrobial Resistance of Commensal Escherichia coli Isolated from Food Animals in Qatar

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