Associations between Interleukin Gene Polymorphisms and Risks of Developing Extremity Posttraumatic Osteomyelitis in Chinese Han Population

Abstract

This case-control study aimed to investigate potential associations between interleukin (IL) gene polymorphisms and the risks of developing extremity posttraumatic osteomyelitis (PTOM) in Chinese Han population. Altogether, 189 PTOM patients and 200 healthy controls were genotyped of IL-1α (rs17561, rs1800587), IL-1β (rs16944, rs1143627, rs1143634, rs2853550), IL-1RN (rs4251961, rs419598, rs315951), IL-4 (rs2243248, rs2243250), IL-6 (rs1800795, rs1800796, rs1800797), IL-8 (rs4073, rs2227306, rs2227307), IL-10 (rs3024491, rs3024496, rs1800871, rs1800872, rs1800896), IL-17A (rs2275913), and IL-17F (rs763780) using the SNaPshot genotyping method. Statistical differences were observed regarding the genotype distributions of rs16944 (P=0.049) and rs4251961 (P=0.007) between the patients and healthy controls. In addition, significant associations were found between rs16944 and the risk of PTOM development by dominant (OR=1.854, P=0.017), homozygous (OR=1.831, P=0.041), and heterozygous (OR=1.869, P=0.022) models, and of rs1143627 by dominant (OR=1.735, P=0.032) and homozygous (OR=1.839, P=0.040) models. Moreover, significant links were also identified between rs4251961 and the susceptibility to PTOM by dominant (OR=0.446, P=0.005) and heterozygous (OR=0.409, P=0.003) models, and of rs1800796 by dominant (OR=4.184, P=0.029), homozygous (OR=4.378, P=0.026), and heterozygous (OR=3.834, P=0.046) models. The present outcomes demonstrated that rs16944, rs1143627, and rs1800796 associate with increased risks, while rs4251961 links to a decreased risk of PTOM development in Chinese Han population.

Genetic Determinants for Bacterial Osteomyelitis: A Focused Systematic Review of Published Literature

“…The result of IL1A rs1800587 regarded as a risk factor for osteomyelitis was consistent with the outcome reported by Asensi et al (2003). However, no significant difference was observed between patients with osteomyelitis and healthy controls regarding the individual alleles and genotypes of IL-1α rs1800587 in the studies of Jiang et al (2020) and Osman et al (2016). Osman et al (2015) have reported that the population with the C allele or CC genotype might increase the susceptibility to hematogenous osteomyelitis, whereas the T allele or CT genotype could act as a protector factor.…”

“…Osman et al (2016) have also revealed that the AA genotype of rs16944 in IL1β could be a risk factor, whereas the allele G and GG genotype of this SNP were considered a protector against hematogenous osteomyelitis among Saudis. However, this result was contrary to the conclusion of the study by Jiang et al (2020). Zhao et al (2020) have recently reported that the mutant allele A in rs2430561 might be a risk factor of posttraumatic osteomyelitis, and individuals with the AT genotype in this gene might have a higher risk of developing posttraumatic osteomyelitis.…”

“…According to our review, rs1143627 and rs1143634 in IL1B, rs1800796 and rs1800795 in IL6, rs2243248 and rs2243250 in IL4, rs1800871 in IL10, rs3212227 in IL12B, rs1800587 in IL1A, rs2430561 in IFNG, rs1799964 in TNF, rs689466 in PTGS2, rs45567233 in CTSG, rs731236 and rs2228570 in VDR, rs1144393 and rs1799750 in MMP1, and rs4646972 in PLAT(t-PA) and BAX-248G/A increased the risk of osteomyelitis, whereas rs4251961 in IL1RN, rs380099 in TLR2, and the CT genotype of rs2070874 in IL4 could protect against osteomyelitis. However, the results of Jiang et al (2020) have suggested that the GG or GA genotype of IL1B rs16944 was a risk factor of osteomyelitis, which was inconsistent with the results of the study by Osman et al (2016). The inconsistencies could be attributed to the insufficient sample size and population from different countries.…”

“…The document selection process is detailed in the flowchart (Moher et al, 2009), shown in Figure 1. Among the 14 included studies, two have reported the associations between SNPs and posttraumatic bacterial osteomyelitis (Wang et al, 2017;Jiang et al, 2020), and two studies have reported the relationship between SNPs and hematogenous bacterial osteomyelitis (Osman et al, 2015(Osman et al, , 2016. Seven studies have reported the associations between SNPs and three types of osteomyelitis (posttraumatic, hematogenous, and vascular insufficiency-related bacterial osteomyelitis; Asensi et al, 2003;Montes et al, 2006;Valle-Garay et al, 2013;Jiang et al, 2016;Hou et al, 2018;Perez-Is et al, 2019;Zhao et al, 2020).…”

“…In seven case-control studies, 14 SNPs in nine genes encoding cytokine-related proteins (IL-1α, IL-1β, IL-1RN, IL-4, IL-6, IL-10, IL-12β, IFN-γ, and TNF-α) were investigated. Recently, Jiang et al (2020) have reported a significant statistical difference in the genotype distribution of IL1B genes rs16944 and rs1143627 between patients with osteomyelitis and controls and revealed that the GG and AG genotypes of rs16944 and the TT and CT genotypes of rs1143627 were linked to the risk of posttraumatic osteomyelitis. Additionally, the CC and CG genotypes of rs1800796 located on the IL6 gene were associated with an increased risk of posttraumatic osteomyelitis.…”