Associations between inflammasome‐related gene NLRP3 Polymorphisms (rs10754558 and rs35829419) and risk of bladder cancer in a Chinese population

Xu, Gang; Huang, Ruohui; Xia, Wei; Jiang, Bo; Xiao, Guancheng; Li, Yanmin

doi:10.1002/jcla.23973

Cited by 10 publications

(8 citation statements)

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“…COPD susceptibility is associated with the G allele and the 459-461 GCA genotype, which can enhance the risk of COPD. However, for the demonstration that GG is the susceptible genotype [11] and other articles [16] have reported higher expression of the GG gene NLRP3, one of the possibilities for the difference in results is the regional variation of samples collected, and the effect of the rs10754558 SNP on COPD remains unknown.In addition, the distribution of SNPs differs between subtypes of COPD and is associated with COPD development. Notable is the fact that genotype 459-461 GCA, as a mutant containing three consecutive bases, has the highest degree of mutation, which can not only trigger the early aggravation of risk but also foretell the development risk from GOLD2 to GOLD3-4.…”

Section: Discussionmentioning

confidence: 99%

The 3’-UTR Polymorphisms in the NLRP3 Gene Associated with the Risk of COPD and Their Putative Effects on the microRNA Mechanism

Yan,

Ting,

Ling

et al. 2023

Preprint

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Objective Evaluating the association between a single nucleotide polymorphism in the 3'UTR region of the miRNAs binding site of the NLRP3 gene and the occurrence and development of Chronic Obstructive Pulmonary Disease (COPD) and providing information to aid in the early detection and treatment of COPD. Methods The regulatory SNPs located in NLRP3 3'UTR region were searched by using dbSNP database and miRNA binding site prediction database. Meanwhile, samples from COPD patients and healthy controls in the same period were used for verification. The clinical baseline information of all subjects was collected, and the transcription level and protein expression level of NLRP3 and the expression level of inflammatory factors downstream of NLRP3 were detected. The effects of SNPs single nucleotide changes on the transcription and expression of inflammatory factors were analyzed. Results The study included 418 participants (249 in the COPD group and 169 in the control group). NLRP3 SNPs with miRNA binding sites include rs10754558 (G>C), rs1664774076 (ATAT>-), and rs1664775106 (C>G). Furthermore, two genotypes, GCG and GCA, were discovered to have a linkage mutation at 3'UTR 459-461. COPD susceptibility is tightly associated to the expression of the rs1664774076 -/- genotype, and the risk of COPD increased by 3.4 times (P≤0.0001). Type 459-461 GCA was substantially related with the likelihood of developing COPD at various stages (P<0.05). Except for rs10754558, all homozygous mutants increased NLRP3 mRNA and protein levels. NLRP3 had the greatest area under the ROC curve for predicting the development and diagnosis of COPD when compared to its downstream inflammatory variables (AUC= 0.9291). Conclusions The NLRP3 rs1664774076 -/- genotype is a COPD susceptibility gene, and the GCA genotype at 459-461 can be used as an early predictor of COPD exacerbation. The NLRP3 3'UTR polymorphism may alter the loss of miRNA binding sites, leading to an increase in NLRP3 expression. In the development of COPD, NLRP3 has a better diagnostic value than traditional inflammatory factors.

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Section: Discussionmentioning

confidence: 99%

The 3’-UTR Polymorphisms in the NLRP3 Gene Associated with the Risk of COPD and Their Putative Effects on the microRNA Mechanism

Yan,

Ting,

Ling

et al. 2023

Preprint

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“…In one report, patients carrying the rs10754558 polymorphism were found to be at a higher risk of developing gastric cancer following Helicobacter pylori infection [ 12 ], and in another report, it was linked to higher bladder cancer risk, particularly among smokers and individuals that drink alcohol. Moreover, in bladder cancer patients rs10754558 has been linked to both tumor size and lymph node metastasis [ 16 ]. In contrast, there have been fewer studies examining the association between the NLRP3 rs10733113 polymorphism and disease.…”

Section: Discussionmentioning

confidence: 99%

Impact of NLRP3 gene polymorphisms (rs10754558 and rs10733113) on HPV infection and cervical cancer in southern Chinese population

Lu,

Lao,

Gan

et al. 2023

Infect Agents Cancer

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Objective Mutations in the NLRP3gene have previously been linked to certain forms of cancer, but there have not been any specific studies examining the association between NLRP3 polymorphisms and cervical cancer (CC). This study was therefore designed to investigate the effect of NLRP3 gene polymorphisms on HPV infection and cervical cancer in southern Chinese population. Methods Multiplex PCR and next-generation sequencing approaches were used to assess the NLRP3 rs10754558 and rs10733113 polymorphisms in 404 cervical lesion patients, including 227 diagnosed with CC and 177 diagnosed with cervical intraepithelial neoplasia(CIN), with 419 healthy female controls being included for comparison. Correlations between the rs10754558 and rs10733113 genotypes and alleles in these patients and CC and CIN were then analyzed. Results No correlations were found between NLRP3 rs10754558 and rs10733113 and human papillomavirus(HPV) infection status. Relative to the healthy control group, the NLRP3 rs10754558 GG genotype, CG + GG genotype, and G allele frequencies were significantly increased among patients with cervical lesions (CC and CIN) (OR = 1.815,P = 0.013;OR = 1.383, P = 0.026; OR = 1.284, P = 0.014,respectively), whereas no such differences were observed for rs10733113. A higher cervical lesion risk was detected for patients over the age of 45 exhibiting the rs10754558 GG genotype (OR = 1.848, P = 0.040). Additionally, the risk of CC was elevated in patients with the rs10754558 GG genotype or the G allele relative to patients with the CC genotype or the C allele(OR = 1.830, P = 0.029; OR = 1.281, P = 0.039). The rs10733113 genotypes or alleles were not significantly associated with CC risk (P > 0.05). No association between rs10754558 and rs10733113 genotypes and CC patient clinicopathological features were observed (P > 0.05). Serum NLRP3, IL-1β, and IL-18 levels were significantly elevated in CC patients relative to healthy controls(P < 0.05). Relative to the CC genotype, CC patients harboring the rs10754558 GG genotype exhibited significantly elevated IL-1β and IL-18 levels(P < 0.05). Conclusion The rs10754558 polymorphism in the NLRP3 gene may contribute to an elevated risk of CC, although it is not significantly correlated with HPV infection and CC progression.

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“…In addition, furthermore, LIAS expression had the ability to forecast the effectiveness of immunotherapy in cancer patients [ 39 ]. The risk of BLCA, tumor size, and lymph node metastasis are all related to the NLRP3 polymorphism [ 40 ]. The low level of NLRP3 in renal cancer suggests that NLRP3 may play a tumor suppressor role in RCC [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

A novel cuproptosis-related lncRNAs signature predicts prognosis in bladder cancer

Wu,

Chen,

Cao

et al. 2023

Aging

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This study constructed a novel cuproptosis-related lncRNAs signature to predict the prognosis of BLCA patients. The Cancer Genome Atlas (TCGA) database was used to retrieve the RNA-seq data together with the relevant clinical information. The cuproptosis-related genes were first discovered. The cuproptosis-related lncRNAs were then acquired by univariate, the least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression analysis to create a predictive signature. An eight cuproptosis-related lncRNAs (AC005261.1, AC008074.2, AC021321.1, AL024508.2, AL354919.2, ARHGAP5-AS1, LINC01106, LINC02446) predictive signature was created. Compared with the low-risk group, the prognosis was poorer for the high-risk group. The signature served as an independent overall survival (OS) predictor. Receiver operating characteristic (ROC) curve indicated that the signature demonstrated superior predictive ability, as evidenced by the area under the curve (AUC) of 0.782 than the clinicopathological variables. When we performed a subgroup analysis of the different variables, the high-risk group’s OS for BLCA patients was lower than that of the low-risk group’s patients. Gene Set Enrichment Analysis (GSEA) showed that high-risk groups were clearly enriched in many immune-related biological processes and tumor-related signaling pathways. Single sample gene set enrichment analysis (ssGSEA) revealed that the immune infiltration level was different between the two groups. Finally, quantitative RT-PCR showed that AC005261.1, AC021321.1, AL024508.2, LINC02446 and LINC01106 were lowly expressed in tumor cells, while ARHGAP5-AS1 showed the opposite trend. In summary, the predictive signature can independently predict the prognosis and provide clinical treatment guidance for BLCA patients.

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Associations between inflammasome‐related gene NLRP3 Polymorphisms (rs10754558 and rs35829419) and risk of bladder cancer in a Chinese population

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 10 publications

References 50 publications

The 3’-UTR Polymorphisms in the NLRP3 Gene Associated with the Risk of COPD and Their Putative Effects on the microRNA Mechanism

The 3’-UTR Polymorphisms in the NLRP3 Gene Associated with the Risk of COPD and Their Putative Effects on the microRNA Mechanism

Impact of NLRP3 gene polymorphisms (rs10754558 and rs10733113) on HPV infection and cervical cancer in southern Chinese population

A novel cuproptosis-related lncRNAs signature predicts prognosis in bladder cancer

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