Association study of interleukin‐1 family, interleukin‐6, and its receptor gene polymorphisms in patients with recurrent aphthous stomatitis

Hollá, Lýdie Izakovičová; Valová, Simona; Linhartová, Petra Bořilová; Bártová, Jiřina; Petanová, Jitka; Kuklı́nek, Pavel; Fassmann, Antonín

doi:10.1111/jop.12594

Cited by 10 publications

(7 citation statements)

References 28 publications

(96 reference statements)

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“…Our preliminary microarray analysis revealed the altered expression of CASC2 in RAS. It has significant correlation with interleukin 6 (IL-6) and interleukin 18 (IL-18), as two critical inflammatory factors in RAS [14]. Therefore, this study was performed to analyze the involvement of CASC2 in RAS.…”

Section: Introductionmentioning

confidence: 99%

LncRNA CASC 2 is upregulated in aphthous stomatitis and predicts the recurrence

Zhang

2020

BMC Oral Health

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Background: Recurrent aphthous stomatitis (RAS) is a common oral disease with unknown molecular pathogenesis. Our preliminary microarray analysis revealed the altered expression of lncRNA Cancer Susceptibility Gene 2 (CASC2) in RAS. We therefore analyzed the role of CASC2 in RAS. Methods: In this study, plasma samples were obtained from RAS patients and healthy participants. Plasma levels of CASC2 were measured by RT-qPCR. Plasma levels of IL-6 and IL-18 were measured by enzyme-linked immunosorbent assay (ELISA). A follow-up study was performed to analyze the role of CASC2 in the recurrence of RAS. Results: In the present study, we found that lncRNA Cancer Susceptibility Gene 2 (CASC2), as well as pro-inflammatory factors interleukin 6 (IL-6) and interleukin 18 (IL-18), were upregulated in plasma of RAS patients compared with healthy participants. Plasma levels of lncRNA CASC2 were positively correlated with plasma levels of IL-6 and IL-18 in RAS patients but not in healthy participants. Compared with pre-treatment levels, plasma levels of lncRNA CASC2, IL-6 and IL-18 were reduced after recovery. A follow-up study showed that patients with high levels of lncRNA CASC2 had a significantly higher recurrence rate. Conclusion: LncRNA CASC 2 is upregulated in RAS and predicts the recurrence.

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Section: Introductionmentioning

confidence: 99%

LncRNA CASC 2 is upregulated in aphthous stomatitis and predicts the recurrence

Zhang

2020

BMC Oral Health

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“…The lesions usually heal spontaneously within 7-10 days without scarring [2][3][4]. Most affected patients usually develop more than one ulcer in each episode, and experience several episodes annually [2] Despite the high prevalence, and the extensive research on RAS, its etiology is still unclear; instead, a number of predisposing factors have been proposed including, but not limited to, genetic predilection, hematologic factors, immunologic defects, vitamins deficiencies, emotional stress, and local trauma [2,3,[5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Single application of topical doxycycline in management of recurrent aphthous stomatitis: a systematic review and meta-analysis of the available evidence

et al. 2020

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Background: Recurrent aphthous stomatitis (RAS) is a highly prevalent oral mucosal disease. The management of RAS is quite challenging, and as yet, there is no definitive cure. The present systematic review and meta-analysis assessed the efficacy of a single application of topical doxycycline for the management of RAS. Methods: A comprehensive online search of PubMed, Scopus, Embase, and Web of Science databases was conducted to identify all relevant studies published up to March 31, 2019. All randomized clinical trials that assessed the efficacy of a single application of topical doxycycline in the management of RAS were included. Primary outcome measures were pain scores and/or healing time, while secondary outcomes included the associated side effects. RevMan 5.3 software was used for data analysis. Results: Five clinical trials fulfilled the eligibility criteria, three of which were included in the meta-analysis. All of the included studies found doxycycline effective in alleviating signs and symptoms of RAS. The results of the pooled 3 studies revealed a statistically significant decrease in the healing time in favor of the doxycycline group as compared to the control groups (I 2 = 51%; MD:-1.77, 95% CI: − 2.11, − 1.42, P <0.00001); however, the results showed no significant differences between doxycycline and control groups with regard to pain reduction (I 2 = 96%; MD:-1.28, 95% CI: − 2.83, 0.27; P = 0.11). Conclusion: Although still inconclusive, the available evidence suggests that a single application of topical doxycycline might be effective for reducing signs and symptoms of RAS. However, owing to the limited number of the included studies, further well-designed clinical trials with adequate sample sizes are required to discern the clinical efficacy of topical doxycycline in patients with RAS.

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“…Despite the rapid development of anticatarrhals and treatment methods, recurrent aphthous stomatitis (RAS) still represents the most common chronic oral disease, which suffered from a long-term painful and characterized by a yellowish ulcer and with an erythematous halo that heals spontaneously [ 1 – 3 ]. The prevalence of RAS could variy from 5% to 25% for people with different diet habits and come to the highest for people between 10-40 years of age [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Systemic bioinformatics analysis of recurrent aphthous stomatitis gene expression profiles

Chen

Shang

et al. 2017

Oncotarget

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Recurrent aphthous stomatitis (RAS) represents the most common chronic oral diseases with the prevalence ranges from 5% to 25% for different populations. Its pathogenesis remains poorly understood, which limits the development of effective drugs and treatment methods. In this study, we conducted systemic bioinformatics analysis of gene expression profiles from the Gene Expression Omnibus (GEO) to identify potential drug targets for RAS. We firstly downloaded the gene microarray datasets with the accession number of GSE37265 from GEO and performed robust multi-array (RMA) normalization with affy R programming package. Secondly, differential expression genes (DEGs) in RAS samples compared with control samples were identified based on limma package. Enriched gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of DEGs were obtained through the Database for Annotation, Visualization and Integrated Discovery (DAVID). Finally, protein-protein interaction (PPI) network was constructed based on the combination of HPRD and BioGrid databases. What’s more, we identified modules of PPI network through MCODE plugin of Cytoscape for the purpose of screening of valuable targets. As a result, 915 genes were found to be significantly differential expression in RAS samples and biological processes related to immune and inflammatory response were significantly enriched in those genes. Network and module analysis identified FBXO6, ITGA4, VCAM1 and etc as valuable therapeutic targets for RAS. Finally, FBXO6, ITGA4, and VCAM1 were further confirmed by real time RT-PCR and western blot. This study should be helpful for the research and treatment of RAS.

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Association study of interleukin‐1 family, interleukin‐6, and its receptor gene polymorphisms in patients with recurrent aphthous stomatitis

Abstract: In conclusion, IL-1 and IL-6 or its receptor gene variants cannot be used as markers for identification of Czech patients with increased risk of recurrent aphthous stomatitis.

Cited by 10 publications

References 28 publications

LncRNA CASC 2 is upregulated in aphthous stomatitis and predicts the recurrence

LncRNA CASC 2 is upregulated in aphthous stomatitis and predicts the recurrence

Single application of topical doxycycline in management of recurrent aphthous stomatitis: a systematic review and meta-analysis of the available evidence

Systemic bioinformatics analysis of recurrent aphthous stomatitis gene expression profiles

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