Association Study and Expression Analysis of Stearoyl Co-A Desaturase as a Candidate Gene for Fatty Acid Composition in Indonesian Crossbred Chickens

Gunawan, Asep; Nurajizah, Eva Siti; Listyarini, Kasita; Furqon, Ahmad; Bilyaro, Woki; Sumantri, Cece; Jakaria, Md.; Akter, Syeda Hasina; Uddin, Muhammad Jasim

doi:10.3923/ijps.2018.348.355

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…In this study, the SNP c.73T>C polymorphism was successfully identified using PCR-RFLP with AciI restriction enzyme (5-C|CGC-3). PCR-RFLP was widely used to identify the gene polymorphism associated with economic traits of animals, such as body compositions, lactation, reproduction, immune system, fatty acids composition, carcass, and meat quality [30][31][32][33]. This study revealed three genotypes of TT, TC, and CC.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of leptin gene (single nucleotide polymorphisms c.73T>C) and its association with body weight and body measurements in Madura cattle

et al. 2022

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Background and Aim: Madura cattle is local cattle in Indonesia. This cattle hasphenotypic variations in growth traits. This study aimed to identify leptin (LEP) gene polymorphism exon 2 associated with body measurements in Madura cattle. Materials and Methods: We recorded body weight (BW) and body measurements of 51 Madura cattle aged 1-4 years in Waru District, Madura. The LEP gene genotyping was conducted using the polymerase chain reaction-restricted fragment length polymorphism method with AciI restriction enzyme. Results: A 267 bp DNA fragment of the LEP gene was successfully amplified using a pair of primers. This study revealed three genotypes (TT, TC, and CC) and two alleles (T and C). The frequencies of TT, TC, and CC genotypes were 0.275, 0.45, and 0.275, respectively, whereas the frequencies of T and C alleles were 0.500 and 0.500, respectively. The c.73T>C mutation was significantly associated with BW, body length (BL), and chest girth (CG) (p<0.05). Among all genotypes, the TC had the highest BW, BL, and CG. Conclusion: Conclusively, LEP gene polymorphism (c.73T>C) exon 2 was polymorphic and associated with body measurements, especially BW, BL, and CG.

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Section: Discussionmentioning

confidence: 99%

Polymorphism of leptin gene (single nucleotide polymorphisms c.73T>C) and its association with body weight and body measurements in Madura cattle

et al. 2022

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RNA deep sequencing reveals novel transcripts and pathways involved in the unsaturated fatty acid metabolism in chicken

et al. 2019

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The genetic quality improvement of native and local chickens to increase production and meat quality in order to build the Indonesian chicken industry

Sumantri

Khaerunnisa

Gunawan

2020

IOP Conf. Ser.: Earth Environ. Sci.

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Native and local chickens have a great effect in the development of the local chicken industry in rural communities. Local chicken industry significantly contributes to the income value of national agricultural development as well as become an important foundation in building food self-sufficient. Indonesia has a wealth of genetic resources of native and local chicken with abundant genetic diversity, but has not been managed and utilized properly. In Indonesia, local chicken agribusiness is less developed due to lack of genetic improvement and has a direct impact with the very low contribution of meat and eggs. Indonesia has a variety of genetic sources for native and local chickens spread throughout the region. Native and local chickens contribute greatly to the rural economy but their production is still limited. Despite its low productivity, native chicken production is very important for rural communities. However, they face challenges how to increase the productivity of their population, which can benefit them financially and increase food security and reach market potential. This native chicken has the characteristics of slow growth and varied egg production. The development of the potential of native chicken and related issues of food security and food independence, local chicken is suitable for this. Information on the performance and productivity of local chickens is needed for local chicken development. Potential local chickens such as pelung chickens are known as singer chickens with a large body frame. Sentul chickens are another potential local chicken, known as meat-producing chickens with good egg production. Through breeding programs (selection and crossing), increasing the productivity of meat and eggs of native chicken can be done effectively. In addition, improving the quality of local chickens can be improved through conventional feeding management. The availability of selected local chicken in accordance to market demand is still limited. To overcome the lack of selected local chicken, the Faculty of Animal Science, IPB has been developing superior local chicken breed since 2012-2018 namely IPB-D1. The up-to-date Next Generation Sequencing technology through RNA Sequencing can identify candidate genes and potential SNPs quickly, thoroughly and comprehensively, as biomarkers for increasing both productivity (egg production, growth rate, and resistant to diseases) and meat quality (tenderness, flavor, meat fiber and fatty acid composition). During the last 6 years (2012-2018), several studies have been conducted on genes related to various trais: (1) disease resistance: toll receptor-4 (TLR4), mycovirus-1 (Mx-1), natural resistance associated macrophage-1 (NRAMP-1), inducible nitric oxide synthase (iNOS), transforming growth factor-β (TGF-β) genes; (2) meat production: growth hormone receptor (GH-r), growth hormone secretagogue receptor (GHSR) genes; (3) meat quality: calpastatine (CAST), calpain (CAPN) and myostatin (MSTN) genes; and (4) composition of fatty acids: stearyol CoA desaturase (SCD) and salute carrier (SLC) genes. Genetic quality of native and local chickens might be improved to increase their production and meat quality in order to build the Indonesian chicken industry.

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Association Study and Expression Analysis of Stearoyl Co-A Desaturase as a Candidate Gene for Fatty Acid Composition in Indonesian Crossbred Chickens

Cited by 3 publications

References 23 publications

Polymorphism of leptin gene (single nucleotide polymorphisms c.73T>C) and its association with body weight and body measurements in Madura cattle

Polymorphism of leptin gene (single nucleotide polymorphisms c.73T>C) and its association with body weight and body measurements in Madura cattle

RNA deep sequencing reveals novel transcripts and pathways involved in the unsaturated fatty acid metabolism in chicken

The genetic quality improvement of native and local chickens to increase production and meat quality in order to build the Indonesian chicken industry

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