Association of TLR2 and TLR4 Gene Single Nucleotide Polymorphisms with Fungal Keratitis in Chinese Han Population

Wang, Ningqian; Zhao, Guopeng; Gao, Ang; Che, Chang-Yan; Qu, Xiaoli; Liu, Y.; Guo, Yanli

doi:10.3109/02713683.2013.827212

Cited by 13 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Різноспрямований характер зв'язку одиночних нуклеотидних поліморфізмів (SNP), які розташовані в генах TLR2 і TLR4, з виникненням грібкового кератиту також показано у популяції Хань [16]. Найбільши зв'язок було показано для rs10983755 TLR4, що розташований у 5'-нетрансльованій області (UTR) гена.…”

Section: результати та їх обговоренняunclassified

TLR4 GENE POLYMORPHISM rs2149356 IN TYPE 2 DIABETES - POSSIBLE RELATIONSHIP WITH DIABETIC MACULAR EDEMA

Ivanyuta

2023

Med. Sci. of Ukr.

View full text Add to dashboard Cite

Background. Polymorphisms in the non-coding regions of the TLR4 gene are known to be associated with the risk of ocular complications of type 2 diabetes (T2DM), with some polymorphisms having no association, others increasing the risk, and some reducing the risk of complications. Aim: to establish the association of the TLR4 gene polymorphism rs2149356 with diabetic retinopathy (DR) and diabetic macular edema (DME) in T2DM. Materials and methods. The study included 81 patients (81 eyes) with T2DM, in whom, according to the guidelines of the American Academy of Ophthalmology (2002), DR and DME were detected, the control group consisted of 50 patients (50 eyes) with T2DM, normalized carbohydrate metabolism, DR 0 (no retinopathy) and absent DME. Genotypes of rs2149356 were determined by real-time polymerase chain reaction using the Gene Amp® PCR System 7500 amplifier (Applied Biosystems, USA) and TaqMan Mutation Detection Assays Life-Technology (USA). MedStat and MedCalc v.15.1 software packages (MedCalc Software bvba) were used for statistical research. Results. In this study, no association of the genetic polymorphism rs2149356 of the TLR4 gene with the development of DR and DME in T2DM was found (p=0.326). Stratification by stages of DR showed no dependence of the distribution of genotypes, while according to the degree of DME, the distribution of genotypes was definitely different in DME 3. Thus, among the carriers of the ancestral genotype G/G, none of DME 3 was found, the borderline level of the total retinal volume (TRV) was 6.7 mm3, above which DME 1 or DME 2 was determined. All carriers of the heterozygote G/T had DME 3, and for carriers of the minor homozygote T/T, two threshold values of TRV were determined: above 6.7 mm3, DME 1 or DME 2 was determined, and higher than 8.7 mm3 – DME 3. Analysis of the relationship between the rs2149356 TLR4 and the phenotype of patients showed greater central retinal thickness and TRV in heterozygous and minor homozygous T/T carriers, which corresponded to greater retinal damage compared to ancestral homozygous G/G carriers (p<0.001). Conclusion. Data were obtained on the association of diabetic retinal damage with the rs2149356 TLR4 – retinal edema was more pronounced in carriers of the T allele.

show abstract

Section: результати та їх обговоренняunclassified

TLR4 GENE POLYMORPHISM rs2149356 IN TYPE 2 DIABETES - POSSIBLE RELATIONSHIP WITH DIABETIC MACULAR EDEMA

Ivanyuta

2023

Med. Sci. of Ukr.

View full text Add to dashboard Cite

show abstract

“…Mucormycosis frequently co-occurs with aspergillosis ( Alfano et al, 2006 ; Tsikala-Vafea et al, 2020 ; Johnson et al, 2021 ; Zayet et al, 2021 ). SNPs in PRRs like Toll-like receptors (TLRs) have already been associated with increased susceptibility to fungal infections ( Bergantim et al, 2013 ; Wang et al, 2013 ; Fischer et al, 2016 ; Zhang et al, 2019 ; Aqsa et al, 2020 ). The TLR2 SNP rs5743708 (R753Q, GA/AA genotype, n = 12) is associated with a higher risk of pneumonia and invasive fungal infections in patients with acute myeloid leukemia undergoing chemotherapy ( Fischer et al, 2016 ).…”

Section: Current Aspects In Diagnosis and Prediction Of Other Fungal ...mentioning

confidence: 99%

“…The TLR2 SNP rs5743708 (R753Q, GA/AA genotype, n = 12) is associated with a higher risk of pneumonia and invasive fungal infections in patients with acute myeloid leukemia undergoing chemotherapy ( Fischer et al, 2016 ). TLR with SNP’s have previously been associated with increased susceptibility to fungal infections ( Wang et al, 2013 ; Fischer et al, 2016 ). TLR2 SNP rs5743708 (R753Q, GA/AA genotype, n = 12) is associated with a higher risk of pneumonia and invasive fungal infections in patients with acute myeloid leukemia receiving chemotherapy ( Fischer et al, 2016 ).…”

Section: Current Aspects In Diagnosis and Prediction Of Other Fungal ...mentioning

confidence: 99%

Mini Review: Risk Assessment, Clinical Manifestation, Prediction, and Prognosis of Mucormycosis: Implications for Pathogen- and Human-Derived Biomarkers

Acosta-España

Voigt

2022

Front. Microbiol.

View full text Add to dashboard Cite

Mucormycosis is a fungal disease caused by members of the fungal order Mucorales, which are abundantly found in terrestrial environments. The fungi propagate clonally via mitospores, which are transmitted to humans through the air and cause superficial or invasive infections. The disease has emerged in recent years and coincides generally with immunosuppression on the patient side. Mucormycosis is still rarely recognized in the clinical because of its unspecific symptoms which often triggers misdiagnosis with bacterial or viral infections leading to prolonged therapeutic cycles and loss of valuable time to manage mucormycosis properly. Infected patients develop various clinical forms, most notably ranging from rhinocerebral via pulmonary to gastrointestinal forms. Traditional diagnosis is based on culture and histopathologic examinations of the affected tissue. But, the achievement of a precise result is time-consuming, labor-intensive, requires mycological expertise and the finding appears often too late. A rapid and precise diagnosis is mandatory because symptoms are non-specific and the disease is rapidly progressing with often fatal outcome. Mucormycosis was increasingly associated with other infections and underlying conditions and risk factors causing comorbidities, which are difficult to successfully manage. This mini-review summarizes the current knowledge on the epidemiology and causative agents of mucormycosis, transmission, risk factors, clinical presentation, diagnosis, and highlights the lack of appropriate biomarkers on the pathogen and the host sides for rapid pathogen and host susceptibility detection, respectively. Fungal antigens and single nucleotide polymorphisms (SNPs) in human host genes are useful for the assessment of susceptibility. This mini-review addresses possibilities for early prediction of susceptibility to mucormycosis based on forecasting of the risk of infection with fungal pathogens other than Mucorales. The topic of early prediction and diagnosis of mucormycosis represents a current research gap and highlights the importance of potential future developments in the area of risk assessment, susceptibility prognosis in conjunction with early diagnosis to reduce mortality in patients suffering from mucormycosis.

show abstract

“…A study among a Han Chinese population of patients with FK compared controls found a particular TLR4 allele that was associated with an increased risk of developing FK [14].…”

Section: Cytokines and Innate Immunitymentioning

confidence: 99%

Fungal Keratitis: Update for 2014

Stone

Tan

2014

Curr Ophthalmol Rep

View full text Add to dashboard Cite

Fungal keratitis is a challenging problem worldwide, and many obstacles remain to the effective prevention, diagnosis, and therapy of this vision-threatening condition. Recent research has highlighted progress in each of these facets of the clinical approach to keratomycosis. Increased awareness of contact lens-associated fungal keratitis has prompted regulators to pay greater attention to testing of contact lens solutions under realworld conditions. Related research has also delved into the mechanisms of fungal biofilm formation and related therapeutic targets. Advances in polymerase chain reaction methodologies promise to increase the rapidity and accuracy of diagnosis, thereby decreasing the delay in instituting appropriate therapy. Antifungal pharmacologic agents remain the first line of therapy, with the newer azole antifungal voriconazole being utilized in innovative ways, such as via intrastromal injection. Large prospective studies such as the Mycotic Ulcer Treatment Trial have helped delineate optimal treatment algorithms, and variations in surgical techniques and postoperative regimens continue to be refined. Natamycin may still be more effective than other recently utilized antifungals for the treatment of Fusarium infections, but treatment failure is still a concern. Post-keratoplasty immunosuppression may benefit from the use of topical calcineurin inhibitors such as cyclosporine and tacrolimus, given their intrinsic antifungal properties. It can be argued that the prevention, diagnosis, and treatment of fungal keratitis lag behind the state of the art for bacterial keratitis, but dramatic improvements are undoubtedly on the horizon.

show abstract

Association of TLR2 and TLR4 Gene Single Nucleotide Polymorphisms with Fungal Keratitis in Chinese Han Population

Abstract: The rs10983755, located in the 5'-untranslated region (UTR) of TLR4, was most strongly associated with FK of Chinese Han population (p < 0.05). The analysis of TLR2 gene polymorphisms showed no significant association upon FK susceptibilities of Chinese Han population.

Cited by 13 publications

References 27 publications

TLR4 GENE POLYMORPHISM rs2149356 IN TYPE 2 DIABETES - POSSIBLE RELATIONSHIP WITH DIABETIC MACULAR EDEMA

TLR4 GENE POLYMORPHISM rs2149356 IN TYPE 2 DIABETES - POSSIBLE RELATIONSHIP WITH DIABETIC MACULAR EDEMA

Mini Review: Risk Assessment, Clinical Manifestation, Prediction, and Prognosis of Mucormycosis: Implications for Pathogen- and Human-Derived Biomarkers

Fungal Keratitis: Update for 2014

Contact Info

Product

Resources

About