Association of the Risk of Dental Caries and Polymorphism of <b><i>MBL2</i></b> rs11003125 Gene in Iranian Adults

Abstract

This case-control study aimed to investigate the effect of rs11003125 in dental caries. For this purpose, a total number of 404 individuals – from Fars Province in Iran – were studied. The technique of this research was the tetra-primer amplification-refractory mutation system (ARMS)-PCR. Dental caries prevalence among the 404 individuals was assessed by counting the number of decayed, missing, and filled teeth. In this research, individuals were divided into two groups: cases (n = 238) and controls (n = 166), and the peripheral blood samples were used to extract the genomic DNA. For genotyping of DNA, the tetra-primer ARMS-PCR method was conducted using specific primer pairs. While examining MBL2 rs11003125 polymorphism, we found significant differences in the genotype frequencies between the case and the control group. The pooled estimates indicated that the GG and GC genotypes of MBL2 rs11003125 polymorphism significantly increased, and therefore caries risk (OR = 2.40, 95% CI = 1.31–4.40, p = 0.004) under the dominant model. These findings suggested that polymorphism in MBL2 gene was associated with dental caries in Iranian adults. Further verification is needed with more ethnic groups and larger sample sizes to determine whether rs11003125 polymorphism is related to dental caries in other regions or not.

An association analysis for genetic factors for dental caries susceptibility in a cohort of Chinese children

“…AQP5 (aquaporin 5) and CA6 (carbonic anhydrase 6) participate in saliva secretion and resistance to decreased pH (Felszeghy et al., 2004; Peres et al., 2010). Genes involved in the innate immune response were also reported to contribute to the susceptibility of dental caries, such as MBL2 (mannose‐binding lectin 2), DEFB1 (defensin β1), and LTF (lactotransferrin) (Doetzer et al., 2015; Mokhtari et al., 2019; Olszowski et al., 2012; Ozturk et al., 2010). The proteins encoded by these genes play a key role in antimicrobial, antiviral, antifungal, and antiparasitic activities in mouth.…”

The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population

“…The present study specified whether the SNP in the lncRNA H19 gene is associated with OSCC in Iranian individuals by the use of tetra-primer ARMS-PCR which is an accurate, reliable, and simple method for genotyping singlenucleotide polymorphisms. This method includes a PCR reaction in a vial with two pairs of primers followed by electrophoresis on agarose gel [29]. Noteworthy, although previous studies have reported the role of H19 in various diseases, the present research is the first to investigate the genetic variation of H19 in association with OSCC in an Iranian population.…”

Gene polymorphisms and serum levels of mannose‐binding lectin in Czech patients with recurrent aphthous stomatitis: A case–control study

“…MBL deficiency has previously been associated with an increased risk of severe respiratory tract infections, such as pertussis 21 . Several studies have also reported an association between MBL2 variants and oral diseases, including dental caries, 22,23 but the latest study has not confirmed these results 24 . On the contrary, gene polymorphism in MBL2 codon 54 has been associated with Behcet disease (BD), a well‐known multisystem disorder characterised by oral ulcers among other symptoms.…”

“…Moreover, the distribution of mannose-binding lectin 2 haplotypes and haplogenotypes was similar in the healthy subjects and patients with recurrent aphthous stomatitis (p > 0.05, OR: 0.75-1. 23).…”