Association of the -2518 A/G Polymorphism of MCP-1 with Breast Cancer in Punjab, North-West India

Abstract

Background: Monocyte chemoattractant protein-1 (MCP-1) is a major chemokine thought to be responsible for monocyte and T-lymphocyte recruitment in acute inflammatory conditions and recruitment of macrophages in tumors. It is also implicated in cardiovascular disease, rheumatoid arthritis and chronic obstructive pulmonary disease. The aim of the present study was to investigate the correlation between MCP-1 -2518 A/G polymorphism and breast cancer risk in patients from Amritsar city of Punjab state in North-West India. Materials and Methods: We screened DNA samples of 200 sporadic breast cancer patients and 200 age and gender matched unrelated healthy individuals for MCP-1 -2518 A/G polymorphism using the PCR-RFLP method. Results: A significantly increased frequency of the GG genotype was observed in patients as compared to controls. Individuals carrying the MCP1 -2518GG genotype had a two fold risk for breast cancer (OR=2.06, 95%CI, 1.06-3.98; p=0.03). Genetic models analysis revealed a significant association between MCP-1 -2518 A/G polymorphism and cancer risk in homozygous co-dominant (OR=2.06, 95%CI, 1.06-3.98; p=0.03) and recessive (OR=1.97, 95%CI, 1.05-3.70; p=0.03) models. Conclusions: We conclude that the GG genotype of the MCP-1-2518 A/G polymorphism is associated with increased risk to breast cancer in Punjab, North-West India.

Reproductive factors and breast cancer risk: A meta-analysis of case–control studies in Indian women

“…The result of the meta-analysis done in this study reveals that modifiable factors such as BMI, age at first pregnancy, breastfeeding, OCP use, parity, and marital status are risk factors of breast cancer in addition to nonmodifiable risk factors. Similar findings are given in Datta et al , Devi et al , Dey et al , Gathani et al , Mathew et al (2008), Meshram and Kulkarni, Mohite and Mohite, Samson et al , Singh and Jangra, Wirth et al , Bhadoria et al , Parameshwari et al , Saxena et al , Jayalekshmi et al , Rao et al , and Babita et al [ 9 10 12 17 20 21 22 23 24 26 28 29 30 31 32 33 ] and others. Marital status and urban residence are not independent factors as they are associated with sedentary lifestyle and dietary pattern.…”

“…[ 20 21 22 23 ] Dietary pattern and use of oral contraceptive pills (OCP) were the controversial risk factor of breast cancer. [ 9 21 24 25 26 27 ] Similarly, Meshram and Kulkarni 2009 and Wirth et al 2014 reported that obese women have lower risk of breast cancer. [ 28 29 ] Considering the aforementioned issues, we conducted a meta-analysis of all case–control studies of breast cancer risk conducted in India during 1991–2018 to find pooled estimates of odds ratio (OR) for the corresponding reproductive and other risk factors in Indian women as compared to controls.…”

Association of MCP‐1 promoter polymorphism with susceptibility to nasopharyngeal carcinoma

“…15 The frequency of rs1024611 GG genotype was reported to be clearly increased in several cancers, such as ovarian, 28,29 colorectal 16 and breast cancer. However, the results in different cancer types have been inconsistent.…”

“…[15][16][17] Interestingly, rs1024611 was also reported to be associated with metastasis of NPC after treatment. [15][16][17] Interestingly, rs1024611 was also reported to be associated with metastasis of NPC after treatment.…”

“…Several single nucleotide polymorphisms (SNPs) within the promoter region of MCP-1 have been reported to be significantly associated with the risk of multiple cancers, especially for rs1024611 (-2578 A/T). [15][16][17] Interestingly, rs1024611 was also reported to be associated with metastasis of NPC after treatment. 18 However, the role of rs1024611 in NPC susceptibility has never been investigated.…”