Association of SNPs on CAPN1 and CAST genes with tenderness in Nellore cattle

Abstract

ABSTRACT. We examined whether single-nucleotide polymorphisms (SNPs) in the calpain (CAPN) and calpastatin (CAST) genes, described from Bos primigenius taurus, are polymorphic in Nellore cattle. We also looked for a possible association of linkage disequilibrium of this polymorphism with tenderness of the longissimus dorsi muscle after 7, 14 and 21 days of postmortem aging in 638 purebred Nellore bulls. Meat tenderness was measured as Warner-Bratzler shear force. Additive and dominance effects were tested for SNPs of the three genotypic classes; the substitution effect was tested for SNPs with missing genotypic classes. Genotypic and gene frequencies were also calculated for the different SNPs. An increase in tenderness was observed from 7 to 21 days; the average values for shear force at 7, 14 and 21 days of aging were 5.92 ± 0.06, 4.92 ± 0.05, and 4.38 ± 0.04 kg, respectively. All markers showed polymorphism, but there was no CC genotype for CAPN316, and few animals showed the AA genotype for CAPN530. The alleles CAPN4751, UOGCAST1, and WSUCAST were found to have additive and dominance effects for shear force at 7, 14 and 21 days, while CAPN316 showed a substitution effect for shear force at

Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle

“…UoGCAST and CAPN4751 seemed to be the most promising markers for meat tenderness in this breed, but their effects on other productive traits, such as feed intake and feed efficiency, have not yet been established. In the current study, the variability for CAPN4751 and UoGCAST in Nellore cattle, previously reported in other studies (Pinto et al, 2010(Pinto et al, , 2011, was confirmed, showing that the allelic and genotypic frequencies seem consistent across Nellore populations. Similarly, the cited authors obtained low frequencies for the CC type of CAPN4751 (3.2%) and well-distributed frequencies across the three genotypes of UoGCAST in agreement with studies in Brahman cattle (White et al, 2005;Casas et al, 2006).…”

“…Some polymorphisms in the calpain (CAPN4753, CAPN4751, CAPN530, CAPN316) and calpastatin (WSUCAST, AF_159246.1:g.2959A>G) genes have been evaluated for their associations with meat tenderness in Nellore cattle (Curi et al, 2009(Curi et al, , 2010Pinto et al, 2010). UoGCAST and CAPN4751 seemed to be the most promising markers for meat tenderness in this breed, but their effects on other productive traits, such as feed intake and feed efficiency, have not yet been established.…”

Determination of Calpastatin Gene Polymorphism in Kivircik Crossbred Ewes by PCR-RFLP Method

“…CAST gene is located on the fifth chromosome of sheep and have significant effect on meat tenderness by inhibiting calpaines in post mortem process (Kawasaki and Kawashima 1996;Juszczuk-Kubiak et al 2009;Khederzadeh 2011). Previous studies have also determined the relations of CAST gene and meat quality and carcass traits in livestock animals (Morris et al 2006;Zhou and Hickford 2008;Pinto et al 2010;Li et al 2013). Morever, many studies reported the associations of CAST gene with average daily weight…”

Polymorphisms in Candidate Genes for Beef Quality in Pinzgau Cattle

“…However, tenderness is a complex trait for breeding programs, because evaluation also depends on how animals are slaughtered. Thus, molecular marker information can be of great usefulness for identification of animals with high genetic value for tenderness and the selection process can be conducted on younger animals, even before birth (Pinto et al, 2010). Up to now, several genetic markers associated with differences in beef tenderness have been reported.…”

“…These markers target two genes corresponding to the most important proteolytic system of skeletal muscle, the calcium-activated neutral protease gene (CAPN1) encoding the large subunit of µ-calpain and the calpastatin gene (CAST) encoding a specific inhibitor of the calpains (Corva et al, 2007). Moreover, the causative mutations in the CAPN and CAST genes have been shown to affect significantly not only beef tenderness but also marbling score (Casas et al, 2006;Morris et al, 2006;Lisa and Di Stasio, 2009;Pinto et al, 2010). The key parameter of beef nutritional quality is intramuscular fat content (IMF).…”