Association of single-nucleotide polymorphisms in TLR7 (Gln11Leu) and TLR9 (1635A/G) with a higher CD4T cell count during HIV infection

TLR7 polymorphism, sex and chronic HBV infection influence plasmacytoid DC maturation by TLR7 ligands

“…In both infections, the variant allele associated with higher disease prevalence and augmented disease progression, and with reduced response to IFNα-therapy for HCV (Oh et al, 2009;Said et al, 2014;Askar et al, 2010;Schott et al, 2007b;Fakhir et al, 2018). In all these studies, effects were most prominent in (mostly rs179008 heterozygous) females and all studies predominantly contained Caucasian individuals.…”

“…WT) allele (Oh et al, 2009). Furthermore, in HIV, the variant allele associated with higher infection rates, viral load and disease progression (Oh et al, 2009;Said et al, 2014). In HCV, the variant was more prevalent among chronic patients as compared to healthy controls and related to a poor IFNα treatment response (Schott et al, 2007a;Askar et al, 2010).…”

Frequency of TLR4 (1063A/G and 1363C/T) polymorphisms in healthy and HIV-infected Omani individuals and their relationship to viral load and T cell count

“…Our results in Omani HIV-infected patients corroborate with the results in the Swiss and Spanish cohorts, as we did not find significant associations between these SNPs and the CD4 count during 1 year of viremia, the nadir CD4 count, the CD4 count when the patient reached VL < 50 copies/mL because of cART, and the ratio of the CD4 count 3 and 6 months after reaching VL < 50 copies/mL because of cART to the last CD4 count before reaching VL < 50 copies/mL and to the nadir CD4. It is important to highlight that associations between a high average CD4 count and SNPs in TLR7 and TLR9 were found in this Omani cohort (Said et al, 2014), which underline the weakness of the influence of the TLR4 SNPs on CD4 T cells during HIV infection. Of note, the average CD8 count during 1 year of viremia was also not associated with these SNPs, as shown for the first time by our results.…”

“…DNA sequencing was performed using PCR products treated as previously described by our group (Said et al, 2014). In brief, shrimp alkaline phosphatase/exonuclease I was used to remove the excess dNTPs and primers.…”

“…The use of the average count to investigate such associations allows a better assessment of the long-term effect of these SNPs as opposed to the use of cross-sectional values (Said et al, 2014). Moreover, we investigated the association of these SNPs with the nadir CD4 count and the CD4 count when the patient reached VL < 50 copies/mL because of cART.…”