1997
DOI: 10.1093/jac/40.6.803
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Association of resistance to trimethoprim/sulphamethoxazole, chloramphenicol and quinolones with changes in major outer membrane proteins and lipopolysaccharide in Burkholderia cepacia

Abstract: Antibiotic treatment options for Burkholderia cepacia infection are limited because of high intrinsic resistance. The problem is complicated by development of cross-resistance between antibiotics of different classes. We isolated antibiotic-resistant mutants by stepwise exposure to chloramphenicol (Chlor) and to trimethoprim/sulphamethoxazole (T/S) for four B. cepacia strains: ATCC13945, Per (clinical isolate), Cas and D4 (environmental isolates). Chlor(r) mutants did not produce chloramphenicol acetyl-transfe… Show more

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Cited by 17 publications
(14 citation statements)
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“…Therefore, we examined whether OMP expression had been altered in our S. enterica serovar Typhimurium mutants. We also investigated the possible modification of the LPS, since it has been shown in E. coli that LPS alterations could affect the correct folding (6) and trimerization (29,30) of porins and also since quinolone resistance has been associated with changes in the LPSs of gram-negative bacteria such as Burkholderia cepacia (26). Cell envelope modifications in both the OMPs and the LPS, possibly in relation to decreased outer membrane permeability, were found in the fluoroquinolone-selected mutants.…”
Section: Discussionmentioning
confidence: 99%
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“…Therefore, we examined whether OMP expression had been altered in our S. enterica serovar Typhimurium mutants. We also investigated the possible modification of the LPS, since it has been shown in E. coli that LPS alterations could affect the correct folding (6) and trimerization (29,30) of porins and also since quinolone resistance has been associated with changes in the LPSs of gram-negative bacteria such as Burkholderia cepacia (26). Cell envelope modifications in both the OMPs and the LPS, possibly in relation to decreased outer membrane permeability, were found in the fluoroquinolone-selected mutants.…”
Section: Discussionmentioning
confidence: 99%
“…Changes in the cell envelope, including loss of outer membrane porins or alterations of the lipopolysaccharide (LPS), can be partially responsible for decreased susceptibility to a wide range of unrelated antibiotics (5,26). Active efflux systems that act synergistically with the outer membrane could have a high level of participation in the intrinsic and the acquired antibiotic resistance of gram-negative bacteria (16,22).…”
mentioning
confidence: 99%
“…However, it does not appear clearly from these studies whether such alterations contributed significantly to decreased outer membrane permeability and consecutive quinolone resistance. The role of lipopolysaccharide composition on the accumulation of quinolones has been studied in several bacteria, but it remains unclear, as contradictory results have been obtained [7,13,22,45,47,62]. It has been hypothesised in quinolone-selected Pseudomonas aeruginosa strains that increased amounts of lipopolysaccharide form a permeability barrier which acts preferentially against hydrophilic quinolones [45].…”
Section: Decreased Outer Membrane Permeabilitymentioning
confidence: 99%
“…However, a proportion of CF patients infected with Bcc can develop "cepacia syndrome," a devastating illness characterized by a fatal acute necrotizing pneumonia that causes rapid and progressive respiratory failure, often leading to the patient's death (22). Intrinsic resistance of Bcc bacteria to many commonly used antibiotics (1) and induction of crossresistance to unrelated antimicrobial agents (40) make it difficult to eradicate these bacteria from CF patients.…”
mentioning
confidence: 99%