Association of Pre-miR-146a rs2910164 Polymorphism with Papillary Thyroid Cancer

Abstract

The incidence rate of papillary thyroid cancer (PTC) has increased over the past decades, but the pathogenesis remains unclear. rs2910164, located in pre-miR-146a, has been studied in PTCs with different ethnicity, but the results were inconsistent. Here we evaluate the association between rs2910164 polymorphism and PTC and investigate the effect of this polymorphism on patients' clinicopathological characteristics. 1238 PTC patients and 1275 controls, all Han population, from Northern China, were included in our study. rs2910164 was genotyped using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS). Analysis of inheritance model was performed using the SNPStats program. Strength of association was assessed by odds ratio (OR) and 95% confidence interval (CI). Overall, no statistical difference in rs2910164 genotype distribution and allelic frequencies between cases and controls was found, and patients with different genotypes had similar clinicopathological characteristics in terms of stage, location, concurrent of benign thyroid tumor, and thyroiditis, while, as the number of G alleles increased, proportion of patients aged ≥45 years and those without metastasis increased (P trend < 0.001 and P trend = 0.003, resp.). However, no association remained significant after Bonferroni correction under any model of inheritance. Our results suggest no association between rs2910164 polymorphism with PTC and patients' clinicopathological characteristics.

Genetic polymorphisms of non-coding RNAs associated with increased head and neck cancer susceptibility: a systematic review and meta-analysis

“…Among these 62, 20 were excluded, such as three studies in which primary head and neck cancer was actually not diagnosed, seven studies that did not investigate ncRNA polymorphisms, six studies that did not analyse the association between genetic polymorphism and cancer risk, and four studies that did not report sufficient data for statistical analysis. Finally, 42 studies [ 28 – 69 ] were considered eligible for this meta-analysis and included.…”

Contributions of polymorphisms in miR146a, miR196a, and miR499 to the development of hepatocellular carcinoma

“…Previous studies have reported a correlation between miR-146a genetic polymorphism and the development of a variety of cancers, such as papillary thyroid cancer, esophageal squamous cell carcinoma, bladder cancer, gastric cancer, and lung cancer (Deng et al, 2015;Liu et al, 2015;Qi et al, 2015;Shen et al, 2015a;Sodhi et al, 2015;Wei et al, 2015;Zhang et al, 2015b). Zhang et al (2015b) carried out a study in a Chinese population with 1238 papillary thyroid cancer patients and 1275 controls, and reported no significant association between the miR-146a polymorphism and the risk of papillary thyroid cancer in the Chinese population.…”

“…Zhang et al (2015b) carried out a study in a Chinese population with 1238 papillary thyroid cancer patients and 1275 controls, and reported no significant association between the miR-146a polymorphism and the risk of papillary thyroid cancer in the Chinese population. Shen et al (2015a) performed a case-control study in a Chinese population with 1400 esophageal squamous cell carcinoma patients and 2185 control subjects, and suggested that the miR-146a variant did not influence esophageal squamous cell carcinoma.…”

Investigating the association of rs2910164 with cancer predisposition in an Irish cohort

“…Considering rs2910164 in particular, in vitro studies have suggested that mature miRNA-146a levels are increased in MCF7 cell lines transfected with pcDNA3.3-miR-146C vs pcDNA3.3-miR-146G, which the authors postulate to be related to increased binding capacity of miR-146a to BRCA1 in the presence of the C-variant ( 33 ). However, we, and other authors, have previously shown that circulating miR-146a levels are reduced in the presence of the C allele in patients with BC ( 58 , 59 ).…”

“…Some authors report an association between the variant in heterozygous (GC), but not homozygous (CC) states and an increased risk of papillary thyroid cancer compared to wild-type genotype (GG) ( 24 ), while others report an association with both heterozygous and homozygous states ( 31 ). Data with respect to this association are conflicting, with other groups failing to show an association with hetero- or homozygous genotypes ( 32 , 33 ). Similarly, there are conflicting reports of the impact of the variant allele on mature miR-146a expression, with some authors reporting reduced expression ( 24 ), and others overexpression ( 34 ).…”