Association of Polymorphisms in the Promoter Region of NOS2A Gene with Primary Knee Osteoarthritis in the Greek Population

Leonidou, Andreas; Lepetsos, Panagiotis; Kenanidis, Eustathios; Macheras, George A.; Tzetis, Maria; Potoupnis, Michael; Tsiridis, Eleftherios

doi:10.7759/cureus.6780

Cited by 2 publications

(3 citation statements)

References 28 publications

(27 reference statements)

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“…NO and iNOS are important regulators of OA, synovial inflammation, and chondrocyte apoptosis in synovial joint tissues (Leonidou et al, 2020 ). To confirm whether pretreatment with PBSL inhibits the generation of NO and/or iNOS, we measured the levels of NO and expression of iNOS in LPS‐induced RAW264.7 cells.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, cotreatment with various concentrations of PBSL and IL-1β (20 ng/mL) did not result in synergetic toxicity (Figure 3b). (Leonidou et al, 2020). To confirm whether pretreatment with PBSL inhibits the generation of NO and/or iNOS, we measured the levels of NO and expression of iNOS in LPS-induced RAW264.7 cells.…”

Section: Pretreatment Of Pbsl Affected the Expression Of Mmps And The...mentioning

confidence: 99%

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Chondroprotective effects of Protaetia brevitarsis seulensis larvae as an edible insect on osteoarthritis in mice

Chun,

Nam,

Lee

et al. 2023

Food Science & Nutrition

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Osteoarthritis (OA) is a common chronic joint inflammatory disease characterized by progressive destruction of the articular cartilage, bone remodeling, and excessive chronic pain. Most therapeutic approaches do not rescue the progression of OA effectively or provide relief of symptoms. Protaetia brevitarsis seulensis larva (PBSL), which is attracting attention, is an edible insect with very high nutritional value and herbal medicine for the treatment of blood stasis, hepatic disease, and various inflammatory diseases. However, the effect of PBSL on OA has not yet been investigated. This study aimed to demonstrate the effects of PBSL water extract on the progression of OA using monosodium iodoacetate (MIA)‐induced mice and SW1353 chondrocytes or murine macrophages. We injected MIA into the intraarticular area of mice following pretreatment with either saline or PBSL (200 mg/kg) for 2 weeks, and then locomotor activity, microcomputed tomography and histopathological analysis, quantitative reverse transcriptase–polymerase chain reaction analysis, and western blot analysis were performed. To determine the molecular effects of PBSL, we used interleukin‐1β (IL‐1β)‐induced SW1353 chondrosarcoma or lipopolysaccharide (LPS)‐stimulated macrophages. Pretreatment with PBSL diminished the symptoms of OA. Physical activity, articular cartilage damage, and the generation of microfractures were rescued by pretreatment with PBSL in the mouse model. Pretreatment with PBSL suppressed the progress of OA through the regulation of articular cartilage degradation genes and inflammation in both in vivo and in vitro models. Our results demonstrated that PBSL has value as edible insect that can be used in the development of functional foods for OA.

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Section: Resultsmentioning

confidence: 99%

Section: Pretreatment Of Pbsl Affected the Expression Of Mmps And The...mentioning

confidence: 99%

Chondroprotective effects of Protaetia brevitarsis seulensis larvae as an edible insect on osteoarthritis in mice

Chun,

Nam,

Lee

et al. 2023

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…Unlike synoviocytes, chondrocytes have the ability to self-produce NO, as evidenced by increased levels of iNOS and NO in articular cartilage tissues. However, chondrocytes from patients without OA do not express iNOS, and experimental OA does not develop in iNOS knockout mice [ 130 ]. Some experiments have shown that NO by itself is not cytotoxic to cultured chondrocytes.…”

Section: Molecular Mechanisms Of Gasotransmitter-dependent Apoptosis ...mentioning

confidence: 99%

The Role of Gasotransmitter-Dependent Signaling Mechanisms in Apoptotic Cell Death in Cardiovascular, Rheumatic, Kidney, and Neurodegenerative Diseases and Mental Disorders

Rodkin

Nwosu

Sannikov

et al. 2023

IJMS

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Cardiovascular, rheumatic, kidney, and neurodegenerative diseases and mental disorders are a common cause of deterioration in the quality of life up to severe disability and death worldwide. Many pathological conditions, including this group of diseases, are based on increased cell death through apoptosis. It is known that this process is associated with signaling pathways controlled by a group of gaseous signaling molecules called gasotransmitters. They are unique messengers that can control the process of apoptosis at different stages of its implementation. However, their role in the regulation of apoptotic signaling in these pathological conditions is often controversial and not completely clear. This review analyzes the role of nitric oxide (NO), carbon monoxide (CO), hydrogen sulfide (H2S), and sulfur dioxide (SO2) in apoptotic cell death in cardiovascular, rheumatic, kidney, and neurodegenerative diseases. The signaling processes involved in apoptosis in schizophrenia, bipolar, depressive, and anxiety disorders are also considered. The role of gasotransmitters in apoptosis in these diseases is largely determined by cell specificity and concentration. NO has the greatest dualism; scales are more prone to apoptosis. At the same time, CO, H2S, and SO2 are more involved in cytoprotective processes.

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Association of Polymorphisms in the Promoter Region of NOS2A Gene with Primary Knee Osteoarthritis in the Greek Population

Cited by 2 publications

References 28 publications

Chondroprotective effects of Protaetia brevitarsis seulensis larvae as an edible insect on osteoarthritis in mice

Chondroprotective effects of Protaetia brevitarsis seulensis larvae as an edible insect on osteoarthritis in mice

The Role of Gasotransmitter-Dependent Signaling Mechanisms in Apoptotic Cell Death in Cardiovascular, Rheumatic, Kidney, and Neurodegenerative Diseases and Mental Disorders

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