Association of polymorphism in MicroRNA 219‐1 with clearance of hepatitis B virus infection

Abstract

Polymorphisms in the primary microRNA region may be associated with natural course of hepatitis B virus (HBV) infection. This study evaluated if the mircoRNA 219-1 (miR-219-1) polymorphism can influence the susceptibility towards persistence of HBV infection and the progression to hepatocellular carcinoma (HCC) in patients with chronic HBV infection. A total of 1,439 individuals having either past or present evidence of HBV infection were enrolled for the study. The subjects were divided into four groups; (1) spontaneous recovery (n = 404), (2) chronic HBV carrier (n = 313), (3) chronic HBV carrier with cirrhosis (n = 305), and (4) hepatocellular carcinoma (n = 417). Genotyping was performed at three polymorphic variants (rs421446, rs107822, and rs213210) in the pri-miRNA region of miR-219-1. The rs421446 T allele was found to be strongly associated with HBV clearance (OR = 0.73, P = 0.0005 in a codominant model and OR = 0.67, P = 0.0009 in a dominant model, OR = 0.69, P = 0.04 in a recessive model, respectively). The rs107822 G allele was also found to be associated with HBV clearance (OR = 0.79, P = 0.008 in a codominant model and OR = 0.72, P = 0.01 in a dominant model, respectively). In haplotype analysis, ht2 (T-G-T) and ht1 (C-A-C) were found to be in significant association with the clearance of HBV. However, no significant association was observed between miR-219-1 polymorphism and the risk of HCC occurrence. This result suggests that polymorphisms in the pri-miRNA region of miR-219-1 might be a genetic factor for HBV clearance after infection.

Association between single nucleotide polymorphisms in MiR219‐1 and MiR137 and susceptibility to schizophrenia in a Chinese population

“…After initial denaturation at 95 °C for 10 min, samples were amplified through 40 cycles (92 °C for 15 s, 60 °C for 1 min). Primer and probe sequences of the two lociwere used according to the description by Guella et al [32] and Cheong et al [33] , respectively. In order to validate the result, 5% PCR products were randomly selected to DNA sequencing.…”

Association of Polymorphism in MicroRNA 604 with Susceptibility to Persistent Hepatitis B Virus Infection and Development of Hepatocellular Carcinoma

“…To serve as primary patients for the case-control study, a total of 1,439 individuals, having either past or present evidence of HBV infection, were recruited from the outpatient clinic of the liver unit and the Center for Health Promotion Center at Seoul National University Hospital, as well as Ajou University Hospital. Spontaneously recovered subjects were defined by a repeated seropositivity for both anti-HBs (antibody to hepatitis B surface antigen) and anti-HBc (antibody to hepatitis B core antigen) of the IgG type without HBsAg ( 10 ). The chronic carrier group was evaluated further for disease progression to cirrhosis or occurrence of HCC.…”

“…Using the Wizard genomic DNA purification kit (Promega, Madison, WI, USA), genomic DNA was extracted from patients' peripheral blood samples. SNP genotyping was performed using the TaqMan® ( 12 ) assay in the ABI prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA, USA), as previously described ( 10 ). Genotyping quality control was performed in 10% of the samples, by duplicate checking (rate of concordance in duplicates=100%).…”

Hepatitis B Virus Infection, MicroRNAs and Liver Disease

“…In a case-control study from Korea, Cheong et al [ 69 ] found that the T allele on the miR-604 gene served as a protective factor for the occurrence of HCC in patients with HBV-related chronic liver disease. In another independent study they found the T allele in the miR-219-1 promoter to be strongly associated with HBV clearance [ 70 ]. On the other hand, Kou et al [ 71 ] found that the miR-196a2C>T polymorphism imparts protection in HBV-related HCC.…”