Hepatitis B Virus Infection, MicroRNAs and Liver Disease

Sarkar, Neelakshi; Chakravarty, Runu

doi:10.3390/ijms160817746

Cited by 45 publications

(44 citation statements)

References 94 publications

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“…Recent studies suggest that miRNAs are abundant in the liver, and modulate a wide range of hepatocellular functions . Further, differential expression of miRNAs has been reported for some clinically important liver diseases, suggesting a close association between their dysregulation and liver diseases . Besides, infection with HBV could also alter the expression patterns of miRNAs in the host .…”

mentioning

confidence: 99%

Global microRNA expression profiling in the liver biopsies of hepatitis B virus–infected patients suggests specific microRNA signatures for viral persistence and hepatocellular injury

Singh¹,

Rooge²,

Varshney³

et al. 2018

Hepatology

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confidence: 99%

Global microRNA expression profiling in the liver biopsies of hepatitis B virus–infected patients suggests specific microRNA signatures for viral persistence and hepatocellular injury

Singh¹,

Rooge²,

Varshney³

et al. 2018

Hepatology

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“…miR-22 is shown to control adult neurogenesis, neuronal migration and dendritic arborization 37 . It is also considered as a serum biomarker for hepatitis B infection 38 . miR-124 expression is linked to both pre and post natal neuronal differentiation 39 .Overexpression of miR-124 suppresses the proliferation of medulloblastoma cells 40 .…”

Section: Discussionmentioning

confidence: 99%

Identification and classification of hubs in miRNA target gene networks in human neural stem/progenitor cells following Japanese encephalitis virus infection

Mukherjee

Akbar

Bhagat

et al. 2019

Preprint

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Micro RNA dysregulation is observed in many viral diseases. RNA viruses modulate host miRNA machinery for their own benefit. JEV, a neurotropic RNA virus has been reported to manipulate several miRNAs in neuron or microglia. However, no report indicates a complete sketch of the miRNA profile of NSPCs contributing to viral persistence; hence being focused in our current study. We performed a miRNA array of 84 miRNAs in human neuronal progenitor cell line and primary neural precursor cells isolated from aborted foetus. Several fold downregulation of hsa-miR-9-5p, hsa-miR-22-3p, hsa-miR-124-3p and hsa-miR-132-3p were found in both of the cells. Subsequently, we screened for the target genes of these miRNAs and looked for the important biological pathways the genes significantly regulate. Then we sorted out the target genes which are involved in two or more than two pathways. We constructed a proteinprotein interaction (PPI) network of the miRNA target genes based on their interaction patterns.A binary adjacency matrix for each gene network was prepared. Different modules or communities were identified in those networks using community detection algorithms.Mathematically, we identified the hub genes by analyzing their degree centrality and participation co-efficient in the network. The hub genes were classified either as provincial (P<0.4) or connector hubs (P>0.4). We validated the expression of hub genes in both cell line and primary cells through qRT-PCR post JEV infection and respective miR-mimic transfection.Taken together, our findings highlight the importance of specific target gene networks of miRNAs affected by JEV infection in NSPCs. ImportanceJEV damages the neural stem/progenitor cell population of mammalian brain. However, JEV induced alteration in miRNA expression pattern of the cell population remains an open question, hence warrants our present study. In this study, we specifically address the down-regulation of four miRNAs and we prepared a protein-protein interaction network of miRNA target genes. We identified two types of hub genes in the PPI network namely connector hubs and provincial hubs. These two types of miRNA target hub genes critically influence the participation strength in the networks and thereby significantly influence up and down regulation in several key biological pathways. Computational analysis of the PPI networks identifies key protein interactions and hubs in those modules which opens up the possibility of precise identification and classification of host factors for viral infection in NSPCs and how RNA viruses modulate host miRNA machinery for their own benefit post JEV infection to the cells.

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“…But even if there are several example of how viral miRNAs are important for infection and replication in different contexts such as HIV (Barichievy et al., 2015) or HBV (Sarkar and Chakravarty, 2015), their role in deregulating human mRNAs in different contexts has not been clearly understood.…”

Section: Discussionmentioning

confidence: 99%

Circulating free xeno‐microRNAs – The new kids on the block

Fabris

Calin

2016

Molecular Oncology

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The role of circulating free microRNAs (cfmiRNAs) as promising tools for cancer screening, prognosis and monitoring of anticancer therapies has been widely studied in the past decades. cfmiRNAs have all the characteristics of the perfect biomarkers owing high stability under storage and handling conditions and being detectable not only in plasma, but in almost all body fluids. Moreover, their levels in plasma are likely to resemble ones in the primary tumor. Recently, viral and plant miRNAs have been found in plasma of healthy individuals through deep sequencing technique, and subsequently the same ones were deregulated in patients. Growing body of literature is recently focusing on understanding the potential cross-kingdom regulation of human mRNAs by miRNAs most likely absorbed with food ingestion. In this article we will review the literature concerning the xenomiRs detected in plasma and their role in influencing cancer onset and progression. XenomiRs could potentially be used not only as early screening tool, but also for patients’ prognosis.

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Hepatitis B Virus Infection, MicroRNAs and Liver Disease

Cited by 45 publications

References 94 publications

Global microRNA expression profiling in the liver biopsies of hepatitis B virus–infected patients suggests specific microRNA signatures for viral persistence and hepatocellular injury

Global microRNA expression profiling in the liver biopsies of hepatitis B virus–infected patients suggests specific microRNA signatures for viral persistence and hepatocellular injury

Identification and classification of hubs in miRNA target gene networks in human neural stem/progenitor cells following Japanese encephalitis virus infection

Circulating free xeno‐microRNAs – The new kids on the block

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