“Association of periodontopathic anaerobic bacterial co-occurrence to atherosclerosis” – A cross-sectional study

Chronic periodontitis is associated with erectile dysfunction. A case–control study in european population

“…Atheroma plaque formation at the intima of medium and small vases would be the result of this dysregulation. Although this is a known mechanism, recent research has strengthened the relationship between periodontopathic anaerobic bacteria and atherosclerosis (Mahalakshmi, Krishnan, & Arumugam, ). Based on this pathogenic mechanism, it has been accepted that CP is a risk factor for cardiovascular disease (CVD), and the extent and severity of CP have been positively associated with acute myocardial infarct size as measured by serum troponin I and myoglobin levels (Marfil‐Alvarez et al., ).…”

Innovative application of nested PCR for detection of Porphyromonas gingivalis in human highly calcified atherothrombotic plaques

“…The median number of cycles was 36 with a big range (30 [8,13,9,32]-60 [31]). The results of the PCR were confirmed by gel only in 9 studies [12,15,19,10,21,27,28,30], by DNA sequencing in 8 studies [4,13,16,20,23,25,26,33] and by melting curve analysis in 4 studies [14,18,22,31].…”

“…processed, or in the case of 18 studies, they were further stored until processing [4,8,9,[12][13][14][15][17][18][19][20][22][23][24][25][26]28,29] and 6 studies did not address the processing methods [11,16,30,31,33,34]. Samples were immediately frozen after their transport in 2 studies [8,28], or kept at −20°C in 10 studies [12,[15][16][17]19,21,22,25,29,34] or at −70°C or lower in 10 studies [8,10,11,24,27,28,[30][31][32]34]. In 3 studies, samples were cultured [9], embedded in paraffin [33] or kept in conditioned medium [4] before b...…”

“…Samples have been mechanically homogenized in only 8 studies [4,11,15,16,23,28,30,32]. For 7 studies [9,10,12,13,24,26,27], homogenization was addressed although details were not provided and for 12 studies [8,14,[17][18][19]21,22,25,29,31,33,34] no details on tissue homogenization were provided.…”

“…None of the studies used the exact same PCR protocol and DNA extraction kit. Seventeen studies implemented conventional qualtitative PCR to amplify DNA [8][9][10]17,[19][20][21][23][24][25][26][27][28][29][32][33][34], 7 used quantitative PCR [4,12,14,18,22,30,31] and 4 used nested PCR [11,13,15,16]. The median number of cycles was 36 with a big range (30 [8,13,9,32]-60 [31]).…”