Association of OLR1 gene polymorphism with live body weight and body morphometric traits in Awassi ewes: short communication

Bioinform Biol Insights

Merzah

et al. 2023

Reproductive traits are affected by many factors, including ovarian function, hormones, and genetics. Genetic polymorphisms of candidate genes are associated with reproductive traits. Several candidate genes are associated with economic traits, including the follistatin ( FST) gene. Thus, this study aimed to evaluate whether the genetic variations in the FST gene are associated with the reproductive traits in Awassi ewes. The genomic DNA was extracted from 109 twin ewes and 123 single-progeny ewes. Therefore, 4 sequence fragments from the FST gene were amplified using polymerase chain reaction (PCR) (exon 2/240, exon 3/268, exon 4/254, and exon 5/266 bp, respectively). For a 254 bp amplicon, 3 genotypes were identified: CC, CG, and GG. Sequencing revealed a novel mutation in CG genotypes c.100C > G. The statistical analysis of c.100C > G showed an association with reproductive characteristics. Ewes carrying the c.100C > G had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing compared with CG and CC genotypes. Logistic regression analysis confirmed that the c.100C > G single-nucleotide polymorphism (SNP) is responsible for decreasing litter size. According to these results, the variant c.100C > G negatively affects the traits of interest and is associated with lower reproductive traits in Awassi sheep. As a result of this study, ewes carrying the c.100C > G SNP have lower litter size and are less prolific.

Section: Methodsmentioning

confidence: 99%

A Novel c.100C > G Mutation in the FST Gene and Its Relation With the Reproductive Traits of Awassi Ewes

Albazi

Bioinform Biol Insights

Merzah

et al. 2023

“… 3 Identifying the genetic mechanisms responsible for lamb reproductive performance is imperative to identify the candidate genes and their causal mutations. 4 , 5 The gremlin 1 (GREM1) gene, 6 the prolactin gene, 7 and the oxidized low density lipoprotein receptor 1 (OLR1) gene 8 have all been found to stimulate reproductive function in Awassi sheep. Another gene, paired-like homeodomain transcription factor 2 ( PITX2 ), has also been found to play a role in livestock reproduction.…”

Section: Introductionmentioning

confidence: 99%

Association of Novel C319T Variant of PITX2 Gene 3’UTR Region With Reproductive Performance in Awassi Sheep

Bioinform Biol Insights

Khazaal

2023

Several genes influence sheep’s reproductive performance, among them the paired-like homeodomain transcription factor 2 ( PITX2) gene. Thus, this study aimed to examine whether the variability within the PITX2 gene is associated with the reproductive performance of Awassi ewes. A total of 123 single-progeny ewes and 109 twin ewes were used to extract genomic DNA. An amplicon of 4 sequence fragments from exons 2, 4, 5 (upstream portion), and 5 (downstream portion) of the PITX2 gene was generated by polymerase chain reaction (PCR), 228, 304, 381, and 382 bp, respectively. Three genotypes of 382 bp amplicons were identified: CC, CT, and TT. Sequence analysis revealed a novel mutation in the CT genotype 319C > T. Statistical analysis revealed that single-nucleotide polymorphism (SNP) 319C > T was associated with reproductive performance. Single-nucleotide polymorphism 319C > T-carrying ewes had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing than those carrying CT and CC genotypes. Based on a logistic regression analysis, it was confirmed that the 319C > T SNP decreased litter size. Ewes with TT genotype produced fewer lambs than ewes with CT and CC genotypes. According to these results, the variant 319C> T SNP negatively affects the reproductive performance of Awassi sheep. Ewes carrying the 319C > T SNP have a lower litter size and are less prolific than those without the SNP.

“…Awassi breed is the most predominant sheep in the Middle East areas that are characterized by a high ability to cope with hardy conditions (Al-Thuwaini, 2021a), with high meat and milk production than the other surrounding breeds in the Middle East (Jawasreh, Al-Amareen, & Aad, 2019;Ajafar, Kadhim, & AL-Thuwaini, 2022a). Several genes of lipid metabolism regulate the fatty acid content of livestock meat (Quiñones, Bravo, Calvo Lacosta, & Sepúlveda, 2017;Ajafar, AL-Thuwaini, & Dakhel, 2022b). Among them, the fatty acid synthase (FASN) gene is used to improve fatty acid components (Shi et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Dr. Novel missense variant L46Q of fatty acid synthase gene and fatty acids content in Awassi sheep

Kareem

2022

Acta Sci. Anim. Sci.

Self Cite

This study was conducted to investigate the association between the polymorphism of the FASN gene with fatty acid content in Awassi sheep. A total of 100 male Awassi sheep between the ages of one and two and a half years old were used in this study. Phenotypic measurement was recorded at slaughter, and from each animal, the longissimus dorsi (LD) muscle samples were taken to analyze the fatty acid profile. Genotyping, sequencing reactions, and in silico tools were performed to confirm the variants in amplified fragments. The result of genotyping revealed two genotypes (AA and AB) of the ovine FASN gene (exon 3). Novel SNP (L46Q) was discovered only within the FASN gene (AB genotype). All utilized in silico tools revealed remarkably deleterious effects for the L46Q on the mutant protein structure, function, and stability. Association analysis revealed that the AB genotype has significantly (p < 0.05) higher levels of animal length and monounsaturated fatty acids (MUFA) with lower amounts of saturated fatty acids (SFA) content than the AA genotype. In conclusion, novel SNP (L46Q) was discovered within the FASN gene (AB genotype), made the animals that has the AB genotype associated with good meat quality traits and this polymorphism may serve as markers for meat quality.