Association of NRAMP1 Polymorphisms with Immune Traits in
Indonesian Native Chickens

Muhsinin, M.; Ulupi, Niken; Gunawan, Asep; Wibawan, I Wayan Teguh; Sumantri, Cece

doi:10.3923/ijps.2016.401.406

Cited by 7 publications

(7 citation statements)

References 12 publications

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“…NRAMP-1 and iNOS gene fragments were amplified at an annealing temperature of 60 o C for 20 seconds using the ESCO Thermocycler machine. This result is in accordance with the result reported by Muhsinin et al (2016), but different from the result reported by Liu et al (2016) and Ramasamy et al (2011) at annealing temperature that has been carried out for the primers of NRAMP-1 exon 11 and iNOS intron 24 gene fragments, each at 64 o C for 90 seconds and 55 o C for 1 minute.…”

Section: Discussionsupporting

confidence: 83%

“…In the iNOS intron 24 gene fragment, it was found high TC genotype frequencies. Different results were reported in Barnevelder and Broiler chickens (Kramer et al, 2003) and in Indonesian local chickens (Muhsinin et al, 2016).…”

Section: Discussionmentioning

confidence: 78%

“…Research results of Muhsinin et al (2016) and Tohidi et al (2013) showed that the genotype of the NRAMP-1 gene CC had a high frequency and that the TT genotype was found to be low in both chickens. In the iNOS intron 24 gene fragment, it was found high TC genotype frequencies.…”

Section: Discussionmentioning

confidence: 96%

“…Identifications of NRAMP-1 and iNOS gene polymorphisms were performed in four stages: DNA extraction, DNA amplification, PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, and DNA fragment visualization (Sambrook et al, 2001). The SNPs and primers for NRAMP1 and INOS were used according to Muhsinin et al (2016). The primers for the NRAMP-1 gene (F: 5'-CAATGAGACGGTGTCTGTGG-3', R: 5'-CCCAGAAGAAATCTCCCTGC -3') and INOS gene (F: 5'-CCAAGGACTTACAGGTGTGG-3', R: 5'-CCAGGATGTTTGGGCTGTTG -3') were annealed at 60°C for 20 sec, respectively.…”

Section: Nramp-1 and Inos Genotypingmentioning

confidence: 99%

“…Also, several studies have shown that there are genes controlling chicken resistance to diseases, such as the NRAMP-1 and iNOS genes (Tohidi et al, 2013). Muhsinin et al (2016) reported that NRAMP-1 and iNOS genes were associated with immune traits in Indonesian local chicken. However, no study reported that NRAMP-1 and iNOS genes were found in selected local chicken.…”

Section: Introductionmentioning

confidence: 99%

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Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens

Ardiyana

Gunawan

Murtini

et al. 2020

Trop. Anim. Sci. J.

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NRAMP-1 and iNOS genes were reported to be associated with a defense mechanism against bacteria and virus infections. This study aimed to identify NRAMP-1 and iNOS genes polymorphisms and their associations with the defense mechanisms against Salmonella enteritidis and Newcastle Disease (ND) in SenSi-1 Agrinak chicken. The present study used a total number of 172 SenSi-1 Agrinak chicken. Identifications of NRAMP-1 and iNOS genes polymorphisms were performed by PCR-RFLP method. NRAMP-1 and iNOS genotypes were associated with immunoglobulin Y (IgY) concentration, specific antibodies against S. enteritidis and ND using General Linear Model (GLM). Immunity characteristics were further grouped into high, medium, and low categories. NRAMP-1|SacI exon 11 and iNOS|AluI intron 24 in SenSi-1 Agrinak chickens were polymorphic. TC genotype has a higher immune response to infectious agents compared to TT and CC genotypes. The frequency of C allele was higher than the T allele in the concentration of immunoglobulin Y (IgY), antibodies titers against S enteritidis and ND. The TC genotype of NRAMP-1 gene was significantly associated with ND antibody titers, and the TT genotype of iNOS was significantly associated with S. enteritidis specific antibody. NRAMP-1 and iNOS genes can be used as potential candidate genes for immune traits in SenSi-1 Agrinak chickens.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 96%

Section: Nramp-1 and Inos Genotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens

Ardiyana

Gunawan

Murtini

et al. 2020

Trop. Anim. Sci. J.

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Molecular associations of gallinacin genes with immune response against Salmonella typhimurium in chickens

et al. 2021

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Polymorphic characterisation of gallinacin candidate genes and their molecular associations with growth and immunity traits in chickens

Saleh

Khalil

Iraqi

et al. 2020

British Poultry Science

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1. Four gallinacin (GAL) genes were assessed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) to characterise these genes in Fayoumi (F) and Rhode Island Red (R) breeds and their crosses of Rhode Island Red × Fayoumi (½R½F) and Fayoumi × Rhode Island Red (½F½R). 2. Genes examined were GAL2, GAL3, GAL4 and GAL5. The molecular associations between the SNPs of the gallinacin genes and body weight, caecal bacterial count and the serum antibody titres of IgA, IgG and IgM were determined. In the R breed, the frequency of TC genotype was higher than TT and CC genotypes for the GAL3 gene. The GG genotype frequency was higher than AA and AG genotypes for the GAL4 gene in the other genetic groups, and the CA genotype frequency was higher than CC and AA genotypes in crosses for the GAL5 gene. 3. In all populations, the frequency of the C allele was higher than the T allele for the GAL3 gene, the G allele was higher than the A allele for the GAL4 gene and the C allele was higher than the A allele for the GAL5 gene. The observed heterozygosity in R, ½R½F and ½F½R was 0.476, 0.375 and 0.158 for the GAL3 gene, 0.458, 0.615 and 0.250 for the GAL4 gene and 0.053, 0.792 and 0.739 for the GAL5 gene, while the expected heterozygosities were 0.490, 0.430 and 0.145 for the GAL3 gene, 0.430, 0.348 and 0.219 for the GAL4 gene and 0.229, 0.478 and 0.496 for the GAL5 gene, respectively. 4. On a molecular level, the genotype TT was significantly higher for body weight than TC and CC genotypes in the GAL3 gene. Birds with the GG genotype had a significantly lower Salmonella typhimurium count than birds with AA genotype in the GAL4 gene. Birds with the genotype AA had higher significant body weights than those with CC and CA genotypes in the GAL5 gene. 5. The results indicated that the GAL3, GAL4 and GAL5 genes are potential candidates for selection programmes to improve S. typhimurium resistance and body weight in chickens.

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Association of NRAMP1 Polymorphisms with Immune Traits in Indonesian Native Chickens

Cited by 7 publications

References 12 publications

Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens

Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens

Molecular associations of gallinacin genes with immune response against Salmonella typhimurium in chickens

Polymorphic characterisation of gallinacin candidate genes and their molecular associations with growth and immunity traits in chickens

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