Association of Neuropeptide-Y (NPY) and Interleukin-1beta (IL1B), Genotype-Phenotype Correlation and Plasma Lipids with Type-II Diabetes

Patel, Roma; Dwivedi, Mitesh; Mansuri, Mohmmad Shoab; Ansarullah,; Laddha, Naresh C.; Thakker, Ami; Ramachandran, A. V.; Begum, Rasheedunnisa

doi:10.1371/journal.pone.0164437

Cited by 29 publications

(30 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since TNFα is shown to be an important regulator of adiponectin multimerization 45 , our observations of increased TNFα, reduced adiponectin transcript and HMW adiponectin levels in obese patients are self-explanatory. We had also reported a rise in IL1β levels in obese diabetic patients 46 , asserting the rise in pro-inflammatory adipokine and drop in anti-inflammatory adipokine in obesity-associated low-grade inflammatory condition. Further, adiponectin levels show sexual dimorphism 47 and our results further confirm this as females in general demonstrated a higher tendency of HMW adiponectin/total adiponectin ratio than males.…”

Section: Discussionmentioning

confidence: 73%

A genetic analysis identifies a haplotype at adiponectin locus: Association with obesity and type 2 diabetes

Palit

Patel

Jadeja

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Adiponectin is a prime determinant of the status of insulin resistance. Association studies between adiponectin (ADIPOQ) gene single nucleotide polymorphisms (Snps) and metabolic diseases have been reported earlier. However, results are ambiguous due to apparent contradictions. Hence, we investigated (1) the association between ADIPOQ Snps: −11377C/G, +10211T/G, +45T/G and +276G/T for the risk towards type 2 diabetes (T2D) and, (2) genotype-phenotype association of these SNPs with various biochemical parameters in two cohorts. Genomic DNA of diabetic patients and controls from Gujarat and, Jammu and Kashmir (J&K) were genotyped using PCR-RFLP, TaqMan assay and MassArray. transcript levels of ADIPOQ were assessed in visceral adipose tissue samples, and plasma adiponectin levels were estimated by qPCR and ELISA respectively. Results suggest: (i) reduced HMW adiponectin/ total adiponectin ratio in Gujarat patients and its association with +10211T/G and +276G/T, and reduced ADIPOQ transcript levels in T2D, (ii) association of the above SNPs with increased FBG, BMI, TG, TC in Gujarat patients and (iii) increased GGTG haplotype in obese patients of Gujarat population and, (iv) association of −11377C/G with T2D in J&K population. Reduced HMW adiponectin, in the backdrop of obesity and ADIPOQ genetic variants might alter metabolic profile posing risk towards T2D.Metabolic Syndrome (MS) is the new wave of diseases that has hit the human population in the last few decadesthe Metabolic Syndrome Era. It has become pandemic and with obesity and type 2 diabetes (T2D) clubbed under the MS umbrella, millions of people around the globe have come under its grip. Though obesity and T2D are ubiquitous, there exists a pattern of prevalence based on ethnicity. A recent report has identified demographic transitions, nutrition and lifestyle in the backdrop of genetic predisposition as the chief factors responsible for the rising trend of obesity associated amongst South Asians 1 . Over accumulation of visceral adipose tissue (AT) has been identified as one of the major driving factors towards T2D. Adipose tissue is an important regulator of metabolic homeostasis by virtue of the adipokines (pro-inflammatory and anti-inflammatory) that it secretes. In obese conditions, the fine-tuned balance between the pro-and anti-inflammatory adipokines gets altered leading to various metabolic disorders 2 . These bioactive peptides act locally and distally to calibrate and fine tune various metabolic pathways. Adiponectin is one such calibrator which is abundantly expressed in white adipose tissue 3 . It circulates in three polymorphic forms, low molecular weight (LMW), moderate molecular weight (MMW) and high molecular weight (HMW). Interestingly, the ratio of plasma HMW adiponectin to total adiponectin is more strongly correlated with plasma glucose levels than any of the forms alone 4 . Adiponectin gene (ADIPOQ/ APM1/GBP28) locus, 3q27, has been strongly associated with a variety of metabolic disorders like-impaired glucose tolerance, obesity, dys...

show abstract

Section: Discussionmentioning

confidence: 73%

A genetic analysis identifies a haplotype at adiponectin locus: Association with obesity and type 2 diabetes

Palit

Patel

Jadeja

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many of these adipokines are crucial in maintaining the status of insulin sensitivity of peripheral tissues viz. adipose tissue and skeletal muscle (Pramanik et al, 2017, Patel et al, 2016Patel et al, 2018;Patel et al, 2019;Rathwa et al, 2019 a & b;Rathwa et al, 2020). In view of this, regulating hyperglycaemia by merely regulating the hepatic glucose out-put or enhancing glucose clearance seem superficial.…”

Section: Discussionmentioning

confidence: 99%

Repurposing statin and L-glutamine: replenishing β-cells in hyperlipidemic T2D mouse model

Patel

Parmar

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

While cases of obesity-induced type 2 diabetes (T2D) are on the upswing, current therapies help only manage the symptoms. Of late, L-glutamine has been implicated in the amelioration of T2D by virtue of its glucagon like peptide-1 (GLP-1) secretagogue property. Alongside, there are mixed reports on adiponectin (insulin sensitizer) potentiating property of statins. We aimed to investigate the effect of pitavastatin (P) and L-glutamine (LG) combination on glycemic control and pancreatic β-cell regeneration in a high-fat diet (HFD)+Streptozotocin (STZ) induced T2D mouse model. C57BL6/J mice treated with HFD+STZ were randomly assigned into four groups: Diabetic control, L-glutamine, Pitavastatin and P+LG. Control group was fed with the chow diet. Significant amelioration in insulin resistance along with plasma glucose, lipid profile, adiponectin levels, and mitochondrial complexes I, II, III activities were observed in P+LG group as compared to HFD+STZ treated group. Phosphoenolpyruvate carboxykinase, glucose 6-phophatase, glycogen phosphorylase, and GLUT2 transcript levels were reduced with increased glycogen synthase transcript levels in liver. Further, the protein levels of Insulin receptor 1-β, pAkt/Akt, and AdipoR1 were restored in the skeletal muscle and a significant increase in islet number as a result of β-cell regeneration and reduced β-cell death were also observed in the combination drug treated group. Thus, L-glutamine and pitavastatin in combination can induce β-cell regeneration and regulate glucose homeostasis to bring about amelioration of HFD+STZ induced T2D.

show abstract

“…The IL-1 gene cluster is located on chromosome 2q13–2q14.1 and is comprised of nine genes: IL-1α , IL-1β , the IL-1 receptor antagonist (IL-1RN) , and six IL-1-like factor genes ( IL-1F5 through IL-1F10 ) [ 31 ]. IL-1β is in a 70–110 kb region of chromosome 2q13–21 and consists of 7 exons and 6 introns [ 32 ]. At least 20 SNPs have been reported in the region of IL-1β , among which rs1143627 was identified as an important functional polymorphism that could influence gene transcription and, therefore, lead to functional changes [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

A Study of IL-1β, MMP-3, TGF-β1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population

Chen

Meng

Qian

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

Primary frozen shoulder (PFS) is a common condition of uncertain etiology that is characterized by shoulder pain and restriction of active and passive glenohumeral motions. The pathophysiology involves chronic inflammation and fibrosis of the joint capsule. Single nucleotide polymorphisms (SNPs) at IL-1β, MMP3, TGF-β1, and GDF5 have been associated with risk of a variety of inflammatory diseases; however, no studies have examined these SNPs with susceptibility to PFS. We investigated allele and genotype frequencies of rs1143627 at IL-1β, rs650108 at MMP-3, rs1800469 at TGF-β1, and rs143383 at GDF5 in 42 patients with PFS and 50 healthy controls in a Chinese Han population. Serum samples from both cohorts were evaluated to determine the expression levels of IL-1β. We found that the IL-1β rs1143627 CC genotype was associated with a decreased risk of PFS compared to the TT genotype (P = 0.022) and that serum IL-1β was expressed at a significantly higher level in the PFS cohort compared to that found in the control group (P < 0.001). Our findings indicated no evidence of an association between rs650108, rs1800469, or rs143383 and PFS. IL-1β is associated with susceptibility to PFS and may have a role in its pathogenesis in a Chinese Han population.

show abstract

Association of Neuropeptide-Y (NPY) and Interleukin-1beta (IL1B), Genotype-Phenotype Correlation and Plasma Lipids with Type-II Diabetes

Cited by 29 publications

References 50 publications

A genetic analysis identifies a haplotype at adiponectin locus: Association with obesity and type 2 diabetes

A genetic analysis identifies a haplotype at adiponectin locus: Association with obesity and type 2 diabetes

Repurposing statin and L-glutamine: replenishing β-cells in hyperlipidemic T2D mouse model

A Study of IL-1β, MMP-3, TGF-β1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population

Contact Info

Product

Resources

About