Association of molecular polymorphism in exon 2 of Ovar-DRB1 gene with weight traits in Iranian Makuie sheep breed

ASHRAFI, Fereshteh; Mardani, Karim; HASHEMI, Ali; Darvishzadeh, Reza; Farhadian, Mohammad

doi:10.3906/vet-1305-46

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…This result is similar with Khan et al, (2012), who found in the CAST gene with MN genotype in Balkhi sheep give daily gain higher than the MM genotype at four months' age. Similar results were repoted by Gorlov et al, (2016) for the CAST gene in Salsk sheep and by Ashrafi et al, (2014) in Iranian Makuie sheep.…”

Section: Molecular Markers and Quantitative Traitssupporting

confidence: 75%

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Specific Genes Affecting Body Weight in Iraqi Awassi Sheep

Al-Barzinji

AMEEN

2020

juod

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The present study was to investigate genotypes of three genes (MSTN, CAST and MHC-DRB1) that related to body weight in Awassi lambs. A total of 52 lambs were used. The DNA concentration and DNA purity (A260/280) was calculated automatically by nanodrop and ranged between 10.53-53.94 ng/μl and 1.51-2.19, respectively. The RFLP-PCR results showed that the CAST and MHC-DRB1genes were polymorphism among individuals while the MSTN gene was monomorphism. Lambs with CC genotype for MHC-DRB1 locus have positive effect on body weight at most ages while lambs with AB genotype for CAST locus gave higher body weight at all ages and higher average daily gain. The lambs with BBCCBB genotypes for the three genes under study gave higher weight at weaning (22.15 kg/lamb) and higher average daily gain (0.197 kg/lamb/day) compared with all other genotypes. These results showed that Awassi lambs have potential for growth traits and the selection process with the aid of molecular technique can play a positive and rapid role for improvement this breed of sheep.

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Section: Molecular Markers and Quantitative Traitssupporting

confidence: 75%

“…The PCR products for the MHC-DRB1 gene were digested with RsaI restriction enzyme (Ashrafi et al, 2014). Samples were migrated on a 2.5 % agarose gel.…”

Section: Pcr-rflp For Mhc-drb1 Genementioning

confidence: 99%

Specific Genes Affecting Body Weight in Iraqi Awassi Sheep

Al-Barzinji

AMEEN

2020

juod

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“…MHC class II genes encode glycoproteins which bind and present extracellular pathogens to the circulating helper T lymphocytes and initiate humoral immunity (Abbas et al., 2010 ). Class II loci have been found to be highly polymorphic and considered as a principal target in genotyping studies aimed to evaluate the association of MHC with immune responses and phenotypic traits (Ali et al., 2019 ; Ashrafi et al., 2014 ; Cinar et al., 2016 ; Larruskain et al., 2010 ; Shen et al., 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…Numerous investigations have indicated the MHC polymorphism and its involvement in genetic resistance to diseases in different sheep populations (Ali et al, 2019;Herrmann-Hoesing et al, 2008;Larruskain et al, 2012;Li et al, 2010;Stear et al, 2006). Correlation of MHC with some production and reproduction features, such as growth, body weight and fertility has also been documented in sheep (Ashrafi et al, 2014;Cinar et al, 2016;Gruszczyńska et al, 2000).…”

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confidence: 99%

Association of Ovar‐DRB1 alleles with innate immune responses in sheep

Esmailnejad

Ganjiani

Hosseini‐Nasab

et al. 2021

Veterinary Medicine & Sci

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Background Major histocompatibility complex (MHC) is the best characterised genetic region associated with adaptive immune responses, including humoral and cell‐mediated immunities. Objectives In this study, the association of MHC class II alleles with inflammatory cytokines and acute‐phase proteins was evaluated in sheep population. Methods Allelic diversity of second exon of ovine DRB1 locus (Ovar‐DRB1.2) was determined in 100 indigenous Iranian Lori‐Bakhtiari fat‐tailed sheep using restriction fragment length polymorphism and direct sequencing methods. The association of DRB1.2 alleles with inflammatory cytokines (interleukin‐1β, IL‐1β; IL‐6 and tumour necrosis factor‐α) and acute‐phase proteins (serum amyloid A, alpha‐1‐acid glycoprotein and haptoglobin) was examined using generalised linear model and multivariate regression analysis. Results Seven distinct RsaI restriction patterns and fourteen alleles were identified in this population. Allele DRB1*2101 showed a negative influence on the IL‐6 response and was associated with lower serum level of IL‐6. DRB1.2 heterozygous individuals also showed higher haptoglobin concentration than homozygotes. Conclusions These results provide additional support for the association between Ovar‐DRB1 alleles and regulation of immune responses in sheep population. Description of MHC polymorphism and its role in the controlling of immune responses will increase our understanding of host–pathogen interactions, and ultimately facilitate the selection of disease‐resistant flocks in genetic breeding programs.

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