Association of mating-type with mycelium growth rate and genetic variability of Fusarium culmorum

Irzykowska, Lidia; Bocianowski, Jan; Baturo‐Cieśniewska, Anna

doi:10.2478/s11535-013-0176-3

Cited by 18 publications

(18 citation statements)

References 46 publications

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“…; Irzykowska et al. ), to our knowledge, this is the first work that sequences SRAP fragments to corroborate the efficacy to this molecular marker to amplify ORF.…”

Section: Resultsmentioning

confidence: 67%

“…Although there are some reports that study fungus genetic variability using the SRAP markers (Sun et al 2006;Pasquali et al 2010;Irzykowska et al 2013), to TSS2a TSa1a TPu1a TJu1a THo1b MICAT01 MICAT06 MICAA01 MICAA02 MICAA03 MICAA04 MICAA05 F18 4/3084/1 4/3084/4 F730 F733 6326 60902 FP0335 FP0378 6402 3107 I1499 6025 6107 6159 P2 P9 P5 6191 6183 887 736 527 718 303 295 P12 P15 P18 P21 P23 TAPO21 TAPO34 CA1 CA3 CA2 CA4 CA5 CA6 CA7 P7 721 522 731 506 61colta11 150sanle17 93payca11 20riogr2 127sorpa7 P20 GE1 GE12 GE13 GE4 GE5 GE9 182I468 183I469 188I474 204I490 198I484 206I492 192I478 GE10 I71 I72 I475 LT030857 LT1427491 LT1516525 LT033580 LT1405491 VE1081 ZW3102 ZW3141 ZW1061 TO1081 TO3021 BO1011 I110 BO2071 GE8 199I485 6947 ZW2101 6408 6410 I109 444 F95 I II IIb IIa IIa2 IIa1 IIa1b IIa1a Fig. 1...…”

Section: Resultsmentioning

confidence: 99%

“…and F. culmorum. In these studies, the authors concluded that SRAP is an efficient technique to identify polymorphisms useful to evaluate variability among individuals (Sun et al 2006;Pasquali et al 2010;Irzykowska et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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SRAP as an Informative Molecular Marker to Study the Fusarium poae Genetic Variability

Dinolfo

Castañares

Stenglein

2014

Journal of Phytopathology

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“…; Irzykowska et al. ), to our knowledge, this is the first work that sequences SRAP fragments to corroborate the efficacy to this molecular marker to amplify ORF.…”

Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

SRAP as an Informative Molecular Marker to Study the Fusarium poae Genetic Variability

Dinolfo

Castañares

Stenglein

2014

Journal of Phytopathology

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“…Several strategies including random amplified polymorphic DNA, inter simple sequence repeats and simple sequence repeats, have been currently used in F. culmorum genotyping (Miedaner et al, 2001;Gurel et al, 2010;Irzykowska et al, 2013;Albayrak et al, 2016). Sequence related amplified polymorphism (SRAP) method uses two primers with nucleotide sequence of conserved coding sites throughout the genomes.…”

Section: Introductionmentioning

confidence: 99%

“…Sequence related amplified polymorphism (SRAP) method uses two primers with nucleotide sequence of conserved coding sites throughout the genomes. The method is powerful tool for genetic mapping and genetic diversity analysis (Li and Quiros, 2001;Irzykowska et al, 2013;Mahmoud, 2016). Moreover reliable and supportive output can be provided for making association between phenotypic characteristics (aggressiveness, linear growth rate etc.)…”

Section: Introductionmentioning

confidence: 99%

Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum

Zümrüt

Tunalı

Kansu

et al. 2018

Turkish JAF Sci.Tech.

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Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR) in cereals worldwide. Since F. culmorum has a great level of genetic diversity and the parasexual stage is assumed for this phytopathogen, characterization of isolates from different regions is significant step in food safety and controlling the HB. In this study, it was aimed to characterize totally 37 F. culmorum isolates from Turkey via sequence related amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 type assay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 and MAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands, respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were of MAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer sets yielded amplicons from all isolates. Genetic similarity values were ranged from 39 to 94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5 kb. Amplicon numbers for individuals were ranged from 1 to 16. According to data obtained from current study, SRAP based genotyping is powerful tool for supporting the data obtained from investigations including phenotypic and agro-ecological characteristics. Findings showed that SRAP-based markers could be useful in F. culmorum characterization.

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Mating type genes and genetic markers to decipher intraspecific variability among Fusarium udum isolates from pigeonpea

Kashyap

Rai

Kumar

et al. 2015

J. Basic Microbiol.

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To ascertain the variability in Fusarium udum (Fu) isolates associated with pigeonpea wilt is a difficult task, if based solely on morphological and cultural characters. In this respect, the robustness of five different genetic marker viz., random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC), BOX elements, mating type locus, and microsatellite markers were employed to decipher intra-specific variability in Fu isolates. All techniques yielded intra-specific polymorphism, but different levels of discrimination were obtained. RAPD-PCR was more discriminatory, enabling the detection of thirteen variants among twenty Fu isolates. By microsatellite, ERIC- and BOX-PCR fingerprinting, the isolates were categorized in seven, five, and two clusters, respectively. Cluster analysis of the combined data also showed that the Fu isolates were grouped into ten clusters, sharing 50-100% similarity. The occurrence of both mating types in Fu isolates is reported for the first time in this study. All examined isolates harbored one of the two mating-type idiomorphs, but never both, which suggests a heterothallic mating system of sexual reproduction among them. Information obtained from comparing results of different molecular marker systems should be useful to organize the genetic variability and ideally, will improve disease management practices by identifying sources of inoculum and isolate characteristics.

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Association of mating-type with mycelium growth rate and genetic variability of Fusarium culmorum

Abstract: Abstract

Cited by 18 publications

References 46 publications

SRAP as an Informative Molecular Marker to Study the Fusarium poae Genetic Variability

SRAP as an Informative Molecular Marker to Study the Fusarium poae Genetic Variability

Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum

Mating type genes and genetic markers to decipher intraspecific variability among Fusarium udum isolates from pigeonpea

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