Association of Listeria monocytogenes LIPI-1 and LIPI-3 marker llsX with invasiveness

Vilchis-Rangel, Rodolfo Erik; Espinoza-Mellado, María del Rosario; Salinas-Jaramillo, Irving Jesús; Martínez-Peña, Marcos Daniel; Rodas-Suárez, Oscar R.

doi:10.1007/s00284-019-01671-2

Cited by 24 publications

(22 citation statements)

References 31 publications

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“…LLS is expressed only under oxidative stress conditions and this confers a better ability in terms of phagosome escape. Therefore, the presence of LIPI-3 is considered responsible for the increased virulence in some strains and is the best candidate to date to explain the greater association of lineage I with human listeriosis [68,69]. Another factor playing a fundamental role in host cells invasion and, in particular, in crossing human intestinal barrier during infection is InlA, encoded by the inlA gene.…”

Section: Discussionmentioning

confidence: 99%

Hypo- and Hyper-Virulent Listeria monocytogenes Clones Persisting in Two Different Food Processing Plants of Central Italy

et al. 2021

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A total of 66 Listeria monocytogenes (Lm) isolated from 2013 to 2018 in a small-scale meat processing plant and a dairy facility of Central Italy were studied. Whole Genome Sequencing and bioinformatics analysis were used to assess the genetic relationships between the strains and investigate persistence and virulence abilities. The biofilm forming-ability was assessed in vitro. Cluster analysis grouped the Lm from the meat plant into three main clusters: two of them, both belonging to CC9, persisted for years in the plant and one (CC121) was isolated in the last year of sampling. In the dairy facility, all the strains grouped in a CC2 four-year persistent cluster. All the studied strains carried multidrug efflux-pumps genetic determinants (sugE, mdrl, lde, norM, mepA). CC121 also harbored the Tn6188 specific for tolerance to Benzalkonium Chloride. Only CC9 and CC121 carried a Stress Survival Islet and presented high-level cadmium resistance genes (cadA1C1) carried by different plasmids. They showed a greater biofilm production when compared with CC2. All the CC2 carried a full-length inlA while CC9 and CC121 presented a Premature Stop Codon mutation correlated with less virulence. The hypo-virulent clones CC9 and CC121 appeared the most adapted to food-processing environments; however, even the hyper-virulent clone CC2 warningly persisted for a long time. The identification of the main mechanisms promoting Lm persistence in a specific food processing plant is important to provide recommendations to Food Business Operators (FBOs) in order to remove or reduce resident Lm.

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Section: Discussionmentioning

confidence: 99%

Hypo- and Hyper-Virulent Listeria monocytogenes Clones Persisting in Two Different Food Processing Plants of Central Italy

et al. 2021

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“…However, those virulence genes were also found in non-outbreak clusters including infection-associated MLST CCs, such as CC1 and CC6 [ 34 ]. Additionally, LIPI-3 ( llsAGHXBYDP , [ 26 ]) was detected in non-outbreak clusters, a genetic island which is linked to increased invasiveness of L. monocytogenes [ 38 ]. Last but not least, pathogenicity is a multifactorial process, and cannot merely be derived from the presence or absence of virulence genes [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Backtracking and forward checking of human listeriosis clusters identified a multiclonal outbreak linked to Listeria monocytogenes in meat products of a single producer

Lüth

Halbedel

Rosner

et al. 2020

Emerging Microbes & Infections

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“…LLS (hemolytic and cytotoxic factor conferring a greater virulence to Lm) is expressed only under oxidative stress conditions and this confers a better ability in terms of phagosome escape. Therefore, the presence of LIPI-3 is considered responsible for the increased virulence in some strains [61,62].…”

Section: Distribution Of Ccs and Cg-mlst Clusters And Their Virulence And Stress Resistance Profilesmentioning

confidence: 99%

Intensive Environmental Surveillance Plan for Listeria monocytogenes in Food Producing Plants and Retail Stores of Central Italy: Prevalence and Genetic Diversity

Centorotola

Guidi

D'Aurizio³

et al. 2021

Foods

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Listeria monocytogenes (Lm) can persist in food processing environments (FPEs), surviving environmental stresses and disinfectants. We described an intensive environmental monitoring plan performed in Central Italy and involving food producing plants (FPPs) and retail grocery stores (RSs). The aim of the study was to provide a snapshot of the Lm circulation in different FPEs during a severe listeriosis outbreak, using whole genome sequencing (WGS) to investigate the genetic diversity of the Lm isolated, evaluating their virulence and stress resistance profiles. A total of 1217 samples were collected in 86 FPEs with 12.0% of positive surfaces at FPPs level and 7.5% at RSs level; 133 Lm isolates were typed by multilocus sequencing typing (MLST) and core genome MLST (cgMLST). Clonal complex (CC) 121 (25.6%), CC9 (22.6%), CC1 (11.3%), CC3 (10.5%), CC191 (4.5%), CC7 (4.5%) and CC31 (3.8%) were the most frequent MLST clones. Among the 26 cgMLST clusters obtained, 5 of them persisted after sanitization and were re-isolated during the follow-up sampling. All the CC121 harboured the Tn6188_qac gene for tolerance to benzalkonium chloride and the stress survival islet SSI-2. The CC3, CC7, CC9, CC31 and CC191 carried the SSI-1. All the CC9 and CC121 strains presented a premature stop codon in the inlA gene. In addition to the Lm Pathogenicity Island 1 (LIPI-1), CC1, CC3 and CC191 harboured the LIPI-3. The application of intensive environmental sampling plans for the detection and WGS analysis of Lm isolates could improve surveillance and early detection of outbreaks.

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Association of Listeria monocytogenes LIPI-1 and LIPI-3 marker llsX with invasiveness

Cited by 24 publications

References 31 publications

Hypo- and Hyper-Virulent Listeria monocytogenes Clones Persisting in Two Different Food Processing Plants of Central Italy

Hypo- and Hyper-Virulent Listeria monocytogenes Clones Persisting in Two Different Food Processing Plants of Central Italy

Backtracking and forward checking of human listeriosis clusters identified a multiclonal outbreak linked to Listeria monocytogenes in meat products of a single producer

Intensive Environmental Surveillance Plan for Listeria monocytogenes in Food Producing Plants and Retail Stores of Central Italy: Prevalence and Genetic Diversity

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