Backtracking and forward checking of human listeriosis clusters identified a multiclonal outbreak linked to
            <i>Listeria monocytogenes</i>
            in meat products of a single producer

Lüth, Stefanie; Halbedel, Sven; Rosner, Bettina; Wilking, Hendrik; Holzer, Alexandra; Roedel, Alice; Dieckmann, Ralf; Vincze, Szilvia; Prager, Rita; Flieger, Antje; Dahouk, Sascha Al; Kleta, Sylvia

doi:10.1080/22221751.2020.1784044

Cited by 31 publications

(33 citation statements)

References 40 publications

(43 reference statements)

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“…Data from these groups are displayed separately to make the changes in trends over time more apparent. The additional case numbers in some quarters of the year (Figure 1) were all associated with large-scale outbreaks (3,6). Successfully identifying and controlling large outbreaks, especially after whole-genome sequencing-based surveillance was introduced, possibly explains why the trend in increases ended after 2017 (9).…”

Section: Discussionmentioning

confidence: 99%

“…Of interest, surveillance data from the United States indicate more listeriosis among women and higher proportions of pregnancy-associated cases (11,13) than in our study. One explanation might be that, in Germany, meat products, more often eaten by men, constitute prominent outbreak vehicles (3,4,6,7), whereas in the United States several outbreaks were caused by nonanimal products or cheese (11). Systematic whole-genome sequence typing of Listeria isolates from patients would aid in detecting and investigating outbreaks.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ongoing High Incidence and Case-Fatality Rates for Invasive Listeriosis, Germany, 2010–2019

Wilking¹,

Lachmann²,

Holzer³

et al. 2021

Emerg. Infect. Dis.

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L isteria monocytogenes infections are primarily foodborne and cause gastrointestinal disease or invasive syndromes among infected persons (1). Because L. monocytogenes is an intracellular pathogen and because invasive listeriosis is the primary manifestation in diagnosed listeriosis, persons with defi cient cell-mediated immunity are at increased risk for its symptoms, including sepsis and meningitis. In addition, infection during pregnancy can lead to chorioamnionitis and fetal infection that can result in miscarriage and stillbirth even 2 months after the mother is exposed. One study found that 44% of patients with non-pregnancyassociated (NPA) listeriosis in Germany had received immunosuppressive therapy ≤3 months before illness onset and another 28% had a coexistent immunocompromising illness, such as diabetes (2). Testing for bacteria in blood cultures or cerebrospinal fl uid (CSF) is recommended for diagnosis.Listeria is ubiquitous in the environment and can produce biofi lms in the food production environment and thus contaminate ready-to-eat (RTE) products, which are typically consumed raw or without further processing. Listeria species grow during shelf life, even at low temperatures, and multiply to concentration levels that make invasive listeriosis and outbreaks more likely. For these reasons, it is suspected that L. monocytogenes exposure is very common but the disease rare. However, in recent years several large outbreaks have been reported in Germany (3-7). The StudyWe analyzed mandatory notifi cation data about invasive listeriosis cases in Germany during 2010-2019 to describe time trends, case-fatality rates, demographic distribution, clinical and diagnostic characteristics, and geographic trends (Appendix, https://wwwnc. cdc.gov/EID/article/27/9/21-0068-App1.pdf). In total, 5,576 listeriosis cases were reported during the 10year study period; 5,064 (91%) of those were NPA and 486 (9%) were pregnancy associated, 241 in mothers and 245 in newborns. Information on disease manifestation was not transmitted for 26 cases. The lowest annual incidence was in 2011 (0.41/100,000 residents) and the highest in 2017 (0.93/100,000 residents); the average for 2010-2019 was 0.69/100,000 residents. We observed a steady increase in cases during 2011-2017, but incidence in 2019 was lower than in previous years. Exceptionally high numbers were reported in the third quarters of 2016, 2017, and 2018 (Figure 1).Among the 5,064 NPA listeriosis case-patients, 2,032 (40%) were female and 3,855 (76%) were >65 years of age (Table 1). Listeriosis among adolescents and children other than newborns is rare (37 cases). Incidence in adults 18-44 years of age is <0.1/100,000 residents, in contrast with incidence among adults ≥85 years of age: 3.99/100,000 residents for men and 2.08/100,000 residents for women. Annual median age of case-patients increased steadily from 72 years of age in 2010 to 77 years of age in 2019.Sources for testing samples included CSF (657, 13%), blood (4,097, 81%), and material from other usually steril...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ongoing High Incidence and Case-Fatality Rates for Invasive Listeriosis, Germany, 2010–2019

Wilking¹,

Lachmann²,

Holzer³

et al. 2021

Emerg. Infect. Dis.

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“…Penicillin, amoxicillin, and co-trimoxazole have also been documented as successful in clinical cases where ampicillin was not an option (Hof, 2004;Pagliano et al, 2017;Temple & Nahata, 2000). Despite Listeria's widespread susceptibility to most antibiotics, there are several reports detailing antibiotic resistance in L. monocytogenes isolates of clinical origin (Charpentier & Courvalin, 1999;Chiaverini et al, 2021;Luth et al, 2020;Morvan et al, 2010;Safdar & Armstrong, 2003). Therefore, monitoring for changes in AMR development in L. monocytogenes is critical to maintaining viable antibiotic treatment options.…”

Section: Listeriosis and Antimicrobials Of Clinical Relevancementioning

confidence: 99%

Probing antimicrobial resistance and sanitizer tolerance themes and their implications for the food industry through the Listeria monocytogenes lens

Bland

Brown

Waite‐Cusic

et al. 2022

Comp Rev Food Sci Food Safe

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The development of antibiotic resistance is a serious public health crisis, reducing our ability to effectively combat infectious bacterial diseases. The parallel study of reduced susceptibility to sanitizers is growing, particularly for environmental foodborne pathogens, such as Listeria monocytogenes. As regulations demand a seek‐and‐destroy approach for L. monocytogenes, understanding sanitizer efficacy and its uses are critical for the food industry. Studies have reported the ability of L. monocytogenes to survive in sanitizer concentrations 10–1000 times lower than the manufacturer‐recommended concentration (MRC). Notably, data show that at MRC and when applied according to the label instructions, sanitizers remain largely effective. Studies also report that variables such as the presence of organic material, application time/temperature, and bacterial attachment to surfaces can impact sanitizer effectiveness. Due to the lack of standardization in the methodology and definitions of sanitizer resistance, tolerance, and susceptibility, different messages are conveyed in different studies. In this review, we examine the diversity of definitions, terminology, and methodologies used in studies examining L. monocytogenes resistance and susceptibility to antimicrobials. Research available to date fails to demonstrate “resistance” of L. monocytogenes to recommended sanitizer treatments as prescribed by the label. As such, sanitizer tolerance would be a more accurate description of L. monocytogenes response to low sanitizer concentrations (i.e., sub‐MRC). Conservative use of word “resistance” will reduce confusion and allow for concise messaging as sanitizer research findings are communicated to industry and regulators.

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“…Also, slaughter line, storage temperature and shelf life considered to be potential risk factors for meat products contamination. More in detail, niches in production of meat products, were responsible for Listeria monocytogenes contamination (Lüth et al, 2020).Storage temperature and selection of starter cultures for dairy products, were reported as risk factors. Furthermore, ripening stage, manufacturing processes and surface biofilms are presented in the articles included.…”

Section: Risk Factorsmentioning

confidence: 99%

Microbiological Factors of Food Quality for Risk Assessments in Europe: A Systematic Review

2022

FSQM

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Public authorities and consumers worldwide are increasingly concern about food quality and safety. Europe has established and suggested management systems in food industry that control hazards in food products. However, outbreak incidents by foodborne pathogens existing until now. European studies that present microbiological risk factors or studies which have conducted microbiological risk assessments about a variety of food products, which represent increased risk of harboring pathogens, are included. By using PRISMA guideline, we searched for the most recent publications from 2017-2021 referring microbiological risk assessments from online databases Scopus, PubMed and Science Direct. From 505 articles initially captured, data was extracted from 84 studies regarding microbiological risk factors in terms of food quality and safety, that are evidenced in European studies. Moreover, information about country of origin, food type, production phase and technology used for detection of pathogens, are also presented. Our results, indicate that quality systems should be further developed in order to control all possible routes of contamination in the supply chain. This work provides information to managers in food industry and scientists for further research regarding microbiological risk assessments.

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Backtracking and forward checking of human listeriosis clusters identified a multiclonal outbreak linked to Listeria monocytogenes in meat products of a single producer

Abstract: Kleta (2020) Backtracking and forward checking of human listeriosis clusters identified a multiclonal outbreak linked to Listeriamonocytogenes in meat products of a single producer,

Cited by 31 publications

References 40 publications

Ongoing High Incidence and Case-Fatality Rates for Invasive Listeriosis, Germany, 2010–2019

Ongoing High Incidence and Case-Fatality Rates for Invasive Listeriosis, Germany, 2010–2019

Probing antimicrobial resistance and sanitizer tolerance themes and their implications for the food industry through the Listeria monocytogenes lens

Microbiological Factors of Food Quality for Risk Assessments in Europe: A Systematic Review

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