Association of ionophores, yeast, and bacterial probiotics alters the abundance of ruminal microbial species of pasture intensively finished beef cattle

Mombach, M. A.; Cabral, Luciano da Silva; Lima, Leni Rodrigues; Ferreira, Daniela Cristina; Pedreira, B. C. e; Pereira, D. H.

doi:10.1007/s11250-021-02617-2

Cited by 6 publications

(5 citation statements)

References 31 publications

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“…The ruminal CH 4 production can be reduced in different ways: (1) by decreasing H 2 availability (dos Santos et al, 2023); (2) by increasing H 2 -consuming pathways, such as the production of compounds that act as H 2 sinks; or (3) by reducing the population of methanogenic Archaea. Although we did not observe propionate changes, our results suggest that the decrease in CH 4 concentration was due to the better efficiency of microbial mass, which decreased the available H 2 for CH 4 production (Mombach et al, 2021).…”

Section: Discussioncontrasting

confidence: 78%

Evaluation of yeast-based additives, as an alternative to ionophores, on rumen fermentation of ruminant diets using an in vitro gas production system

Cagliari,

Magnani,

Rigon

et al. 2023

Front. Anim. Sci.

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IntroductionThe study aimed to assess yeast-based additives' effects, as monensin alternatives, on rumen fermentation parameters, greenhouse gas emissions, and ruminal kinetics of ruminant diets using an in vitro system. Three experiments were conducted, each individually evaluating escalating levels of three yeast-based additives.MethodsThree experiments were designed: Experiment 1 evaluated prebiotic blend 1—yeast culture [Saccharomyces cerevisiae (Scer)], beta-glucans, fructooligosaccharides, galactooligosaccharides, and mannanoligosaccharides; Experiment 2 investigated prebiotic blend 2—beta-glucan fractions and mannanoligosaccharides from Scer; Experiment 3 examined yeast cells—hydrolyzed, inactivated, and spray-dried yeast (Scer) cells. Uniform experimental design and procedures were employed across the three experiments. Each experiment had six treatments: monensin (Rumensin®, 25 mg/kg DM) as positive control, and yeast additive levels (0, 533, 1,067, 1,600, and 2,133 mg/kg on DM basis) added to ruminant diets (60% corn silage and 40% concentrate). An in vitro gas production (GP) system with 50 AnkomRF bottles assessed total GP (at 24 and 48 hours), kinetics, fermentation profiles, methane (CH4), and carbon dioxide (CO2) concentrations. Comparison with monensin utilized Dunnett’s test (5%). Yeast additive levels were analyzed for linear and quadratic responses.ResultsIn Experiment 1, the 1,600 mg/kg yeast additive had lower concentrations of propionate, isobutyrate, valerate, and branched-chain volatile fatty acids (BCVFAs), and a higher acetate concentration and acetate-to-propionate ratio than monensin. In Experiment 2, the 1,600 mg/kg yeast additive led to lower total VFA and isovalerate concentrations than monensin. Additionally, compared to the 1,067 mg/kg yeast additive, monensin showed lower isovalerate concentration and higher NH3-N concentration. In Experiment 3, the 533 mg/kg yeast additive resulted in lower valerate and BCVFA concentrations, and higher CH4 and CO2 concentrations than monensin. Monensin had lower total VFA, butyrate, and acetate-to-propionate ratio, and higher propionate concentration compared to the 2,133 mg/kg yeast additive.DiscussionCollectively, these findings suggest yeast-based additives could be monensin alternatives, enhancing animal nutrient utilization efficiency and contributing to improved livestock sustainability.

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Section: Discussioncontrasting

confidence: 78%

Evaluation of yeast-based additives, as an alternative to ionophores, on rumen fermentation of ruminant diets using an in vitro gas production system

Cagliari,

Magnani,

Rigon

et al. 2023

Front. Anim. Sci.

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“…Although S. bovis enhances the degradation of simple sugars and starches, previous research has indicated that Lactobacillus species are the dominant microorganisms adhering to barley and maize grain particles in the rumen (Yang et al, 2018). Consequently, it is plausible that the supplemented LAB strains could compete with S. bovis for the substrates, leading to the observed reduction in its population, a phenomenon consistent with previous findings (Mombach et al, 2021). In addition, Morovský et al (1998) reported that the growth of S. bovis was inhibited by a bacteriocin produced by E. faecium BC25.…”

Section: Discussionsupporting

confidence: 88%

“…This was most likely due to fact that S. ruminantium has a more diverse substrate range, including starch, pectins and proteins when compared to M. elsdenii (Counotte and Prins, 1981). The size of S. ruminantium population also remained stable in a previous study when intensively finished beef cattle was supplemented with a combination of yeast and bacterial probiotics (Mombach et al, 2021). On the other hand, the abundance of S. ruminantium in the present study was higher in the Al.…”

Section: Discussioncontrasting

confidence: 45%

“…On the other hand, both LAB supplementations led to a decrease in the counts of S. bovis and M. elsdenii cells. S. bovis is an amylolytic lactic acid bacterium that grows more readily when animals are fed concentrate-rich diets, and it causes rumen acidosis (Mombach et al, 2021). Although S. bovis enhances the degradation of simple sugars and starches, previous research has indicated that Lactobacillus species are the dominant microorganisms adhering to barley and maize grain particles in the rumen (Yang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

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Preliminary study of the effects of Apilactobacillus kunkeei EIR/BG-1 and Enterococcus hirae EIR/CM-2 supplementation on in vitro rumen fermentation and microbial population

Demirtas,

Sevin,

Musa

et al. 2023

J. Anim. Feed Sci.

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“…(2005), which consists of using sample-derived standards, while real-time PCR analysis was performed according to Mombach et al (2021), using StepOnePlus™ (Applied Biosystems, Carlsbad, CA, USA) equipment with a uorescence detection system using ROX as a reference dye using primers presented in Table 1.…”

Section: Dna Extraction and Qpcr Runsmentioning

confidence: 99%

Effect of concentrate levels on the abundance of some rumen organisms in feedlot Nellore cattle determined by qPCR

Costa

Carvalho

Vieira

et al. 2023

Preprint

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This study aimed to evaluate the effects of concentrate levels (CL) (45 to 90% on a dry matter basis) on the abundance of some species of cultured-rumen bacterial, protozoa, and Archaea in four rumen-fitted Nellore cattle. Species-designated primers were used to quantify rumen bacteria, while group and Domain-specific primers were used to quantify protozoa and Archaea by qPCR. CL caused a tendency of quadratic effect (p = 0.067) in the abundance of Fibrobacter succinogenes, while the abundance of Ruminococcus albus (p = 0.003) and Selenomans ruminantium (p = 0.006) presented a linear increase according to CL. Prevotella ruminicola abundance was quadratically affected by CL (p = 0.005), and Streptococcus bovis demonstrated a tendency of linear decrease (p = 0.076). There was no effect of CL in the abundance of Archaea (p = 0.248), but there was a quadratic effect in the abundance of protozoa (p = 0.033). The increase in CL from 45 to 90% for feedlot cattle promoted significant changes in the abundance of some species of bacteria and protozoa. However, these changes were more abrupt than reported in the literature because the changes in the diets were made progressively. Thus, a progressive increase in CL seems to cause few disturbances in the abundance of cultured rumen bacteria.

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Association of ionophores, yeast, and bacterial probiotics alters the abundance of ruminal microbial species of pasture intensively finished beef cattle

Cited by 6 publications

References 31 publications

Evaluation of yeast-based additives, as an alternative to ionophores, on rumen fermentation of ruminant diets using an in vitro gas production system

Evaluation of yeast-based additives, as an alternative to ionophores, on rumen fermentation of ruminant diets using an in vitro gas production system

Preliminary study of the effects of <i>Apilactobacillus kunkeei</i> EIR/BG-1 and <i>Enterococcus hirae</i> EIR/CM-2 supplementation on<i> in vitro</i> rumen fermentation and microbial population

Effect of concentrate levels on the abundance of some rumen organisms in feedlot Nellore cattle determined by qPCR

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