Association of Interleukin-10 Cytokine Expression Status with HLA Non-DRB1*02 and<i>Mycobacterium bovis</i>BCG Scar-Negative Status in South Indian Pulmonary Tuberculosis Patients

Dheenadhayalan, Veerabadran; Shanmugalakshmi, S.; Vani, S.; Muthuveeralakshmi, P.; Arivarignan, G.; Nageswari, A.D.; Pitchappan, R. M.

doi:10.1128/iai.69.9.5635-5642.2001

Cited by 5 publications

(5 citation statements)

References 42 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high-risk allele HLA DRB1*15 also has a bearing on BCG vaccination-induced immunity and long-term memory as produced by PPD-recalled cytokine responses [21]. Another pathway of tuberculosis susceptibility seems to be associated with a combination of PPD-specific IL-10 cytokine expression, HLA non-DR2 status,and BCG scar-negative status.…”

Section: Community Genetics As a Tool To Investigate Differential Dismentioning

confidence: 99%

Castes, Migration, Immunogenetics and Infectious Diseases in South India

Pitchappan

2002

Public Health Genomics

View full text Add to dashboard Cite

It has been said that the grandest genetic experiment of nature has been conducted in south India in the name of the caste system. One can expect the frequency of an infectious disease to be equal to the product of the frequencies of various indicated loci/alleles, whether physiological, hormonal or immunological, in an endemic area. The sympatrically isolated caste and sub-caste populations of southern India, with differing origins, migration patterns and breeding habits, differ significantly in their HLA and other immune repertoire and are ideal models to study and test this hypothesis. The prevalence of a number of major infectious diseases, including TB and leprosy, are reviewed in different communities in the light of their genetic history.

show abstract

Section: Community Genetics As a Tool To Investigate Differential Dismentioning

confidence: 99%

Castes, Migration, Immunogenetics and Infectious Diseases in South India

Pitchappan

2002

Public Health Genomics

View full text Add to dashboard Cite

show abstract

“…Thus, the disease status was essentially linked to specific TCR V␤ usage (models 1 and 2, 12 TCR V␤ families) and HLA class II high-risk allele status (model 1, five TCR V␤ families). Previous studies have shown that HLA-DR2 status, as well as HLA non-DR2, BCG scarnegative status and IL-10 expression, are associated with pulmonary tuberculosis (4,7,24). In the present study we identified a role for TCR V␤ usage in tuberculosis susceptibility and protection operating in the context of HLA high-risk allele status and within the parameters of BCG vaccination and environmental exposure.…”

Section: Discussionmentioning

confidence: 49%

“…M. bovis hsp65 peptide-specific T-cell clones express more TCR V␤ families 5.1 and J␣ 9 (15,31). Previous studies of cytokine expression in the peripheral blood of tuberculosis patients and endemic controls have shown that there is PPD-specific enhancement or suppression of gamma interferon and IL-10 following 48 h of culture (7). The reason for the observed PPDrecalled expression of many TCR V␤ families in the peripheral blood of more endemic controls than patients in the present study can thus be attributed to the constant exposure to typical ); B, BCG scar status (positive or negative); D, disease status (healthy or diseased).…”

Section: Discussionmentioning

confidence: 99%

“…Samples (3 to 5 ml) of peripheral blood were obtained from the patients and controls in heparin vacutainers (455 051; Greiner, Frickenhausen, Germany) and were transferred to the laboratory. Whole-blood cultures were set up on the same day (7). Briefly, 50 l of whole blood, diluted to a volume of 200 l with RPMI 1640 medium (31870-025; Life Technologies, Gibco-BRL, Gaithersburg, Md.)…”

Section: Study Population (I) Pulmonary Tuberculosis Patientsmentioning

confidence: 99%

“…Furthermore, expression of the Th2 cytokines interleukin-10 (IL-10) and IL-4 is associated with Mycobacterium bovis BCG scar-negative non-DR2 status in patients (7). It has also been demonstrated that there is a spectrum of immune reactivity (delayed-type hypersensitivity versus serum antibodies) in hospital contacts and healthy individuals in southern India, and this finding has been confirmed in other countries where tuberculosis is highly endemic, such as Indonesia and Brazil (3,12,22).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Mycobacterium bovisBCG Scar Status and HLA Class II Alleles Influence Purified Protein Derivative-Specific T-Cell Receptor Vβ Expression in Pulmonary Tuberculosis Patients from Southern India

Shanmugalakshmi¹,

Dheenadhayalan²,

Muthuveeralakshmi

et al. 2003

Infect Immun

Self Cite

View full text Add to dashboard Cite

Purified protein derivative (PPD) RT23-recalled T-cell receptor (TCR) V␤ expression was studied in the peripheral blood of 42 pulmonary tuberculosis patients and 44 healthy controls from southern India, a region where tuberculosis is endemic. Forty-eight-hour whole-blood cultures in the presence or absence of PPD-RT23 were set up, and at the end of the culture period total RNA was extracted and cDNA was synthesized. Expression of various TCR V␤ families was assessed by using family-specific primers. PPD-specific expression (usage) of TCR V␤ families 4, 6, 8 to 12, and 14 was found in more controls than patients. Among the responders (individuals who showed PPD-specific expression), endemic controls had significantly higher responses than the patients had for TCR V␤ families 2, 3, 7, 13, and 17. The majority of the patients did not show usage of most of the TCR V␤ families, and this was attributed to T-cell downregulation. A four-way nested classification analysis revealed that TCR V␤ family 1, 5, 9, 12, and 13 usage in the context of HLA class II high-risk alleles (DRB1*1501, DRB1*08, and DQB1*0601) and Mycobacterium bovis BCG scar status were the determining factors in susceptibility and resistance to tuberculosis. The healthier status of controls was attributed to the wider usage of many TCR V␤ families readily recalled by PPD, while the disease status of the patients was attributed to TCR V␤ downregulation and the resultant T-cell (memory cell?) unresponsiveness. Host genetics (HLA status) and BCG vaccination (scar status) seem to play important roles in skewing the immune response in adult susceptibility to pulmonary tuberculosis through TCR V␤ usage.

show abstract

Not all the infected develop the disease — A “Lotus and Cactus” model

Pitchappan

2016

Infection, Genetics and Evolution

View full text Add to dashboard Cite

Association of Interleukin-10 Cytokine Expression Status with HLA Non-DRB1*02 andMycobacterium bovisBCG Scar-Negative Status in South Indian Pulmonary Tuberculosis Patients

Cited by 5 publications

References 42 publications

Castes, Migration, Immunogenetics and Infectious Diseases in South India

Castes, Migration, Immunogenetics and Infectious Diseases in South India

Mycobacterium bovisBCG Scar Status and HLA Class II Alleles Influence Purified Protein Derivative-Specific T-Cell Receptor Vβ Expression in Pulmonary Tuberculosis Patients from Southern India

Not all the infected develop the disease — A “Lotus and Cactus” model

Contact Info

Product

Resources

About