Association of<i>Ureaplasma diversum</i>with reproductive disease in cattle

Díaz, José Manuel; Prieto, Alberto; López, Gonzalo; Díaz, Pablo; López, C.; Arias, Luis Angel Quintela; Morrondo, P.; Fernández, Gonzalo

doi:10.1080/00480169.2019.1623733

Cited by 16 publications

(13 citation statements)

References 17 publications

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“…Ureaplasma presented a relative abundance higher than 30%. Ureaplasma diversum has been previously reported in bovine vaginal microbiota in several countries including Brazil (26,27), France (28), Canada (29), Costa Rica (30), Argentina (31), Australia (32), and Spain (33). Particularly in Canada, U. diversum has been identified in the microbiota of the reproductive tract of cattle without clinical signs of granular vulvitis (29,34).…”

Section: Discussionmentioning

confidence: 97%

“…Particularly in Canada, U. diversum has been identified in the microbiota of the reproductive tract of cattle without clinical signs of granular vulvitis (29,34). In Spain, the presence of U. diversum was related to granular vulvovaginitis and subclinical endometritis (33). Interestingly, the abundance of Ureaplasma has been reported to be high in the uterine microbiota of cows that failed to become pregnant after 200 days in milk (35).…”

Section: Discussionmentioning

confidence: 99%

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Vaginal Microbiota Changes During Estrous Cycle in Dairy Heifers

et al. 2020

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The vaginal microbiota plays an important role in the health of dairy cattle, and it could be manipulated for the prevention and treatment of reproduction-related infections. The present study profiles and compares the vaginal microbiota of healthy dairy heifers during the estrous cycle focusing the results in follicular (estrus) and luteal (diestrus) phases using 16S rRNA sequencing of the V3-V4 hypervariable region. Twenty 13-16-months-old virgin dairy heifers from a single farm were included in this study. Vaginal swabs and blood samples were obtained during estrus (6-8 h before artificial insemination) and diestrus (14 days after insemination). Estrus was evaluated by an activity monitoring system and confirmed with plasma progesterone immunoassay. Results showed that the taxonomic composition of the vaginal microbiota was different during the follicular and luteal phases. At the phylum level, the most abundant bacterial phyla were Tenericutes, Firmicutes, and Bacteroidetes which comprised more than 75% of the vaginal microbiota composition. The next more abundant phyla, in order of decreasing abundance, were Proteobacteria, Actinobacteria, Fusobacteria, Epsilonbacteraeota, and Patescibacteria. Together with Tenericutes, Firmicutes, and Bacteroidetes represented more than 96% of the bacterial composition. Ureaplasma, Histophilus, f_Corynebacteriaceae, Porphyromonas, Mycoplasma, Ruminococcaceae UCG-005, were the most abundant genera or families. The results also showed that the vaginal microbiota of dairy heifers was non-lactobacillus dominant. The genus Lactobacillus was always found at a low relative abundance during the estrous cycle being more abundant in the follicular than in the luteal phase. Despite more research is needed to explore the potential use of native vaginal microbiota members as probiotics in dairy heifers, this study represents an important step forward. Understanding how the microbiota behaves in healthy heifers will help to identify vaginal dysbiosis related to disease.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Vaginal Microbiota Changes During Estrous Cycle in Dairy Heifers

et al. 2020

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“…Although it grows readily in culture, M. bovigenitalium is not always considered in the differential diagnosis of reproductive disease. This may also be true for U. diversum which is associated with infertility and abortion cases, with in vitro evidence supporting invasion of bovine spermatozoids [ 45 , 46 ], but again lack of awareness of Ureaplasma spp. as a potential aetiological agent, and possibly costs associated with testing, may mean submission of samples and subsequent diagnosis is infrequent.…”

Section: Discussionmentioning

confidence: 99%

Identification of Mycoplasma species and related organisms from ruminants in England and Wales during 2005–2019

2021

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Background Mycoplasma species have been associated with economically important diseases affecting ruminants worldwide and include contagious bovine pleuropneumonia (CBPP), contagious caprine pleuropneumonia (CCPP) and contagious agalactia, listed by the World Organisation for Animal Health (OIE). The Mycoplasma Team at the Animal and Plant Health Agency provides an identification service for Mycoplasma and Ureaplasma species of veterinary importance to the United Kingdom (UK), supporting the detection of new and emerging pathogens, as well as contributing to the surveillance of endemic, and the OIE listed diseases exotic to the UK. Mycoplasma and other Mollicutes species were identified from diagnostic samples from farmed ruminants in England and Wales using a combination of culture and 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis, submitted between 2005 and 2019. Results A total of 5578 mollicutes identifications, which include mycoplasmas and the related acholeoplasmas and ureaplasmas, were made from farmed ruminant animals during the study period. Throughout the study period, the pathogen Mycoplasma bovis was consistently the most frequently identified species, accounting for 1411 (32%) of 4447 molecular identifications in cattle, primarily detected in the lungs of pneumonic calves, followed by joints and milk of cattle showing signs of arthritis and mastitis, respectively. M. bovirhinis, M. alkalescens, M. dispar, M. arginini and Ureaplasma diversum, were also common. Mixed species, principally M. bovis with M. alkalescens, M. arginini or M. bovirhinis were also prevalent, particularly from respiratory samples. The non-cultivable blood-borne haemoplasmas Candidatus ‘Mycoplasma haemobos’ and Mycoplasma wenyonii were identified from cattle, with the latter species most often associated with milk-drop. M. ovipneumoniae was the predominant species identified from sheep and goats experiencing respiratory disease, while M. conjunctivae preponderated in ocular samples. The UK remains free of the ruminant mycoplasmas listed by OIE. Conclusions The continued high prevalence of M. bovis identifications confirms its ongoing dominance and importance as a significant pathogen of cattle in England and Wales, particularly in association with respiratory disease. M. ovipneumoniae has seen a general increase in prevalence in recent years, notably in coughing lambs and should therefore be considered as a primary differential diagnosis of respiratory disease in small ruminants.

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“…However, it is not clear which species of Ureaplasma plays a potential role in swine respiratory disease. To elucidate this, the OTU sequences clustered to Ureaplasma in our results were aligned with the public 16S rRNA genes of bacterial species, and they were found to be most similar to U. diversum , an opportunistic pathogen [ 33 ]. To further validate the presence of U. diversum in infected piglets, we used PCR and sequencing.…”

Section: Resultsmentioning

confidence: 99%

Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing

et al. 2020

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Background and Aim: Due to the incomplete development of the immune system in immature piglets, the respiratory tract is susceptible to invasion by numerous pathogens that cause a range of potential respiratory diseases. However, few studies have reported the changes in pig lung microorganisms during respiratory infection. Therefore, we aimed to explore the differences in lung environmental microorganisms between healthy piglets and piglets with respiratory diseases. Materials and Methods: Histopathological changes in lung sections were observed in both diseased and healthy pigs. Changes in the composition and abundance of microbiomes in alveolar lavage fluid from eleven 4-week-old Chinese Kele piglets (three clinically healthy and eight diseased) were studied by IonS5TM XL sequencing of the bacterial 16S rRNA genes. Results: Histopathological sections showed that diseased pigs displayed more lung lesions than healthy pigs. Diseased piglets harbored lower bacterial operational taxonomic units, α-diversity, and bacterial community complexity in comparison to healthy piglets. Taxonomic composition analysis showed that in the diseased piglets, the majority of flora was composed of Ureaplasma, Mycoplasma, and Actinobacillus; while Actinobacillus, Sphingomonas, and Stenotrophomonas were dominant in the control group. The abundance of Ureaplasma was significantly higher in ill piglets (p<0.05), and the phylogenetic tree indicated that Ureaplasma was clustered in Ureaplasma diversum, a conditional pathogen that has the potential to affect the swine respiratory system. Conclusion: The results of this study show that the microbial species and structure of piglets' lungs were changed during respiratory tract infection. The finding of Ureaplasma suggested that besides known pathogens such as Mycoplasma and Actinobacillus, unknown pathogens can exist in the respiratory system of diseased pigs and provide a potential basis for clinical treatment.

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Association ofUreaplasma diversumwith reproductive disease in cattle

Cited by 16 publications

References 17 publications

Vaginal Microbiota Changes During Estrous Cycle in Dairy Heifers

Vaginal Microbiota Changes During Estrous Cycle in Dairy Heifers

Identification of Mycoplasma species and related organisms from ruminants in England and Wales during 2005–2019

Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing

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