Association ofMMP-1-1607 1G/2G (rs1799750) polymorphism with primary knee osteoarthritis in the Greek population

Abstract

Osteoarthritis is the most common form of arthritis with still unknown pathogenic etiology and considerable contribution of genetic factors. One of the mechanisms of cartilage degradation in osteoarthritis is enzymatic proteolysis of the extracellular matrix by metalloproteinases. MMP-1, produced by chondrocytes and synovial cells, is a major proteinase of the MMPs family. The present study aims at evaluating the association of MMP1 gene -1607 1G/2G (rs1799750) polymorphism with primary knee osteoarthritis in the Greek population. One hundred fifty five patients with primary symptomatic knee osteoarthritis participated in the study along with 139 controls. Genotypes were determined using PCR-RLFP technique. Allelic and genotypic frequencies were compared between both study groups. There was no significant association between MMP1 -1607 1G/2G polymorphism and knee osteoarthritis, in crude analysis; however, after multiple logistic regression analysis, 1G/2G was associated with reduced odds of knee osteoarthritis by 75% in males, compared to genotypes 1G/1G þ 2G/2G, adjusting for age and BMI (adjusted OR: 0.25, 95% CI: 0.069, 0.910, p ¼ 0.035). The present study shows that MMP1 -1607 1G/2G (rs1799750) polymorphism might be a risk factor for knee osteoarthritis susceptibility in the Greek population. Further investigations are needed to confirm this association in the pathogenesis of osteoarthritis. ß

High MMP-1, MMP-2, and MMP-9 protein levels in osteoarthritis

“…MMP-1, MMP-2, and MMP-9 are key members of the MMP family. MMP-1, which is a collagenase, plays a crucial role in cancer progress and metastasis (Lepetsos et al, 2014). MMP-2, which is localized in cardiomyocytes, can degrade type-IV collagen and other bioactive molecules, and it is overexpressed in head and neck squamous cell carcinoma during cancer invasion and metastasis (Hughes and Schulz, 2014).…”

Association Between The Polymorphism of MMP-1 Gene with Knee Osteoarthritis Risk: A Meta-Analysis

“…Recent studies have shown that some functional polymorphisms of MMPs, including MMP-1 (-1607 1G/2G) (rs1799750) polymorphism, have been associated with the risk of knee OA [11][12][13][14][15][16].However, due to the limited sample size of these studies, previous single studies may not be effective, so con icting research results have been obtained, especially considering the heterogeneity of the study and different races, clinical heterogeneity and genetic diversity of other causes. Therefore, we conducted this meta-analysis to provide more con dent evidence.…”

Association between the polymorphism of MMP-1 gene with knee osteoarthritis risk: a meta-analysis

“…Recent studies have shown that some functional polymorphisms of MMPs, including MMP-1 (-1607 1G/2G) (rs1799750) polymorphism, have been associated with the risk of knee OA [11][12][13][14][15][16].However, due to the limited sample size of these studies, previous single studies may not be effective, so conflicting research results have been obtained, especially considering the heterogeneity of the study and different races, clinical heterogeneity and genetic diversity of other causes. Therefore, we conducted this meta-analysis to provide more confident evidence.…”

“…Several studies investigated the relationships of MMP-1 (-1607 1G/2G)(rs1799750) polymorphism with increased knee OA risk in different countries and draw different conclusions [11,[13][14][15][16]. Therefore, we conducted a meta-analysis to assess the effect of the MMP-1 (-1607 1G/2G)(rs1799750) polymorphism on the risk of knee OA.…”