Association ofCDKN2B-AS1rs1333049 with Brain Diseases: A Case-control Study and a Meta-analysis

Abstract

ObjectiveCDKN2B-AS1 polymorphisms were shown to associate with the risk of stroke in European. The goal of this study was to evaluate the contribution of CDKN2B-AS1 rs1333049 to the risk of hemorrhagic stroke (HS) and brain tumor (BT) in Han Chinese.MethodsA total of 142 HSs, 115 BTs, and 494 controls were included in the current association study. The genotyping test was performed using the melting temperature shift method.ResultsWe failed to validate the association of CDKN2B-AS1 rs1333049 with the risk of brain disease. Significantly higher levels of low-density lipoprotein cholesterol (LDL-C) (p=0.027), high-density lipoprotein cholesterol (HDL-C) (p<0.001) and total cholesterol (TC) (p<0.001) were found in HSs in the genotype GG/GC carriers, but not the genotype CC carriers (p>0.05). The meta-analysis of 10 studies among 133,993 individuals concluded that rs1333049 of CDKN2B-AS1 gene was likely to increase a 16% incidence rate of cerebrovascular disease (CD) among various populations (odds ratio 1.16, 95% confidence interval 1.08–1.25; p<0.0001, random-effect method).ConclusionOur case-control study identified rs1333049 genotypes showed different association with the concentration of the LDL-C, HDL-C and TC in the HS patients. Meta-analysis supported the association between rs1333049 and CD risk in various populations, although we were unable to observe association between rs1333049 and the risk of HSs in Han Chinese.

<p>LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p</p>

“…CDKN2B anti-sense RNA (CDKN2B-AS1) is located within the CDKN2B-CDKN2A gene cluster at chromosome 9p21. 21 CDKN2B participates in the development of many diseases such as coronary heart disease (CHD), 22 myocardial infarction, 23 stroke, 24 brain disease, 25 and inflammatory bowel disease in Koreans. 26 The association between CDKN2B-AS1 and cancers is less explored.…”

Integrative metabolomics as emerging tool to study autophagy regulation

“…protein precipitate). Examples where lysis or extraction steps are omitted are solid state NMR spectroscopic studies of intact tissue and solution NMR spectroscopic studies of urine or cerebrospinal fluid 7 54 . In case of MS, samples are measured either directly from the matrix or using extraction protocols to enrich the metabolites of interest 15 .…”

“…The metabolome provides a snapshot of the functional endpoint of complex biological networks and accurately describes the functional and physiological states of an organism 3 4 5 6 . Aiming at in-depth characterization of complex metabolite mixtures, the recent technological developments in the field of metabolomics have opened up a wide range of research fields in biological, biomedical, environmental, agricultural, and nutritional research 7 8 . In biomedical research, metabolomics has established itself as a key technique for systems biology, disease diagnostics, and biomarker discovery 2 9 10 11 12 .…”

Genetic polymorphisms of diabetes‐related genes, their interaction with diabetes status, and breast cancer incidence and mortality: The Long Island Breast Cancer Study Project

“…In this population‐based sample, of the 143 diabetes risk variants genotyped, 12 SNPs were associated with the risk of developing BC, three with all‐cause mortality, and three with BC‐specific mortality, in additive genotype models at an alpha of 0.05, but none were statistically significant at the Bonferroni‐corrected alpha of 0.0003. The top three most significant SNPs associated with BC risk included: rs4876369, an intron variant of SLC30A8 (solute carrier family 30 member 8), which encodes a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles; rs11187146, an intergene variant in the IDE ‐ KIF11 ‐ HHEX gene cluster at 10q23.33; and rs1333049, an intergene variant in the cyclin‐dependent kinase inhibitor 2A/B ( CDKN2A/B ) locus at 9p21.3, hypothesized to play a pivotal role in the development of cardiovascular disease by altering the dynamics of vascular cell proliferation . ORs per allele increase for the 12 statistically significant SNPs ranged from 1.14 to 1.34 for those positively associated with BC and from 0.81 to 0.83 for those inversely associated with BC risk.…”