Association of HTTLPR and 5-HT2A T102C polymorphisms with smoking characteristics and anthropometric profiles of Thai males

Abstract

ABSTRACT. Nicotine increases serotonin release in the brain. Gene polymorphisms in the serotonergic system have been suggested to be associated with smoking behavior. We investigated a possible association between two polymorphisms in the serotonergic system -HTTLPR of a serotonin transporter gene and 5-HT 2A at position T102C -with biochemical and anthropometric parameters, and with cigarette smoking in an investigation of 200 smokers and 111 non-smokers. The two polymorphisms, HTTLPR and 5-HT 2A at position T102C, were genotyped by PCR-RFLP. They were not significantly associated with smoking status in these Thai males. Among the smokers, thiocyanate concentrations and quantity of cigarettes smoked (cigarette pack-years) were significantly higher for individuals with LL/LS genotypes than SS HTTLPR and 5-HT 2A T102C polymorphisms and smoking genotypes of 5-HTTLPR (all P < 0.05), whereas "age at starting smoking" and "duration of smoking" were not significantly different between these two genotypes. Moreover, anthropometric variables, comprising triceps skinfold thickness, arm circumference, waist circumference, hip circumference, and waist-to-hip ratio, were significantly higher for the CC/TC genotypes of 5-HT 2A than the TT genotype (all P < 0.05), except for body mass index. HTTLPR and 5-HT 2A T102C polymorphisms were not significantly associated with smoking status among Thai males; however, the HTTLPR polymorphism among smokers appears to be an indicator of increased smoking intensity consisting of cigarette packyears and thiocyanate concentrations. The 5-HT 2A T102C polymorphism plays a role in the anthropometric profiles, triceps skinfold thickness, arm circumference, waist circumference, hip circumference, and waist-to-hip ratio, but not smoking status in Thai subjects.

Lack of Association between Serotonin Transporter (5-HTT) and Serotonin Receptor (5-HT2A) Gene Polymorphisms with Smoking Behavior among Malaysian Malays

“…Similar to the findings for 5-HTTLPR, our findings for the 5-HT2A polymorphism were consistent with several previous studies that found no association between the polymorphisms in the 5HT gene and smoking behavior [ 23 – 25 ]. A study on a Brazilian population found that the 5-HT2A polymorphism was related to smoking behavior; in particular, the CC genotype was more frequently found in current smokers than in former smokers or individuals who had never smoked [ 21 ].…”

“…Interestingly, the opposite finding was obtained in Caucasian Australians; in this study, the occurrence of the TT genotype was associated with current smokers [ 22 ]. However, this finding has not been replicated in several other studies in different populations [ 23 – 25 ], indicating that the influence of these genes may vary from one ethnicity to another.…”

“…Another study involving a Thai population also found no significant association between 5-HTTLPR polymorphisms and smoking behavior. However, individuals who carried the L allele were found to have higher-intensity smoking behaviors [ 25 ].…”

Increased lipid peroxidation and lowered antioxidant defenses predict methamphetamine induced psychosis

“…These findings extend previous findings that MA use increases MDA and other markers of lipid peroxidation in the blood and brain and decreases catalase, Gpx, GSH, SOD, and thiols groups (Mirecki, Fitzmaurice et al 2004, Moszczynska, Fitzmaurice et al 2004, Fitzmaurice, Tong et al 2006, Govitrapong, Boontem et al 2010, Suriyaprom, Tanateerabunjong et al 2011, Huang, Lin et al 2013, Hacimusalar, Karaaslan et al 2019. MA also causes mitochondrial oxidative damage in human T lymphocytes (Potula, Hawkins et al 2010) and in vivo and in vitro MA exposure increases ROS production in the CNS (Pubill, Chipana et al 2005, Wu, Ping et al 2007.…”

Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study

“…To date, many gene candidates, such as D2 dopamine receptor ( Munafò et al , 2009 ), SLC6A3 dopamine transporter ( Vandenbergh et al , 2002 ), BDNF ( Suriyaprom et al , 2013 ) and HTTPLRF ( Suriyaprom et al , 2012 ), have been analyzed. However, the results are inconclusive, often due to the relatively low numbers of individuals included in the studies.…”