Association of Genetic Polymorphisms in Matrix Metalloproteinase-9 and Coronary Artery Disease in the Chinese Han Population: A Case–Control Study

Abstract

Objective: Matrix metalloproteinase-9 (MMP-9) plays an important role in inflammation and matrix degradation involved in atherosclerosis and plaque rupture. The T allele of rs3918242 has been reported to lead to a high promoter activity and associate with the extent of coronary artery disease (CAD). And some studies have reported that the G allele of rs17576 might be associated with CAD. The aim of this study was to assess the association between the polymorphisms of the MMP-9 gene and CAD in the Chinese Han population. Methods: This case-control study comprised 258 CAD cases and 153 controls from the Chinese Han Population. The genomic DNA of MMP-9 was isolated from whole blood. Polymerase chain reaction-based restriction fragment length polymorphism was used to determine the rs3918242 and rs17576 genotypes in the MMP-9 gene and the total serum levels of MMP-9 were measured using enzyme-linked immunosorbent assay in both case and control groups. Results: Analysis of MMP-9 gene polymorphisms showed that the frequencies of the T allele and CT + TT genotypes of rs3918242 were significantly higher in the case group than in the control group ( p < 0.05). However, the distribution of variant genotypes of rs17576 did not differ between the case and control groups ( p > 0.05). The total serum level of MMP-9 was significantly higher in the case group than in the control group ( p < 0.05). The subjects carrying T alleles in the CAD group had higher average serum MMP-9 levels compared with CC genotypes ( p < 0.05). Conclusions: Our results suggest that the single-nucleotide polymorphism of rs3918242 in the MMP-9 gene is associated with CAD and high serum levels of MMP-9 are also associated with CAD in the Chinese Han population. Therefore, genetic variation of rs3918242 may participate in the development of CAD through influencing MMP-9 expression.

Plasma concentration, genetic variation, and gene expression levels of matrix metalloproteinase 9 in Iranian patients with coronary artery disease

“…studies. [61013] However, some other studies identified no close association between MMP9 -1562C>T polymorphism and plasma levels of MMP9 that were inconsistent with our study. [112526] The explanation behind these contradictory findings may be related to use of some medications such as statins by CAD patients that can change plasma levels of MMP9 as indicated by some studies.…”

“…[1011121314] Our study demonstrated that -1562C>T polymorphism of MMP9 gene didn't increase the risk of CAD either in heterozygote, homozygote, dominant or recessive genetic models. Therefore, our study was in accordance with the Opstad et al .…”

Relationship between serum cytokines receptors and matrix metalloproteinase 9 levels and coronary artery disease

“…Soluble interleukin receptors 2 and 6 (SIL2R, SIL6R) are released into the blood stream in response to the inflammation. 8 It has been suggested that MMP-9 is involved in plaque destabilization and rupture. MMPs also play an important role in proliferation, migration, differentiation, angiogenesis, and apoptosis.…”

Association of COL4A3 (rs55703767), MMP-9 (rs17576)and TIMP-1 (rs6609533) gene polymorphisms with susceptibility to type 2 diabetes

“…MMPs regulate numerous normal and pathological activities (16). The MMP-9 gene is located on chromosome 20q12.2-13.1 and contains 13 exons and 12 introns (17). The activities of MMPs are regulated by tissue inhibitors of metalloproteinases (TIMPs) (18).…”