Association of Eu(III) and Cm(III) onto an extremely halophilic archaeon

Bader, Miriam; Moll, Henry; Steudtner, Robin; Lösch, Henry; Drobot, Björn; Stumpf, Thorsten; Cherkouk, Andrea

doi:10.1007/s11356-019-04165-7

Cited by 13 publications

(12 citation statements)

References 44 publications

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“…TRLFS measurements can help determine the microbial functional groups involved in An/Ln-microorganism interaction. The complexation of Cm(III) with carboxylic functional groups from the archaeon Halobacterium noricense DSM15987 T was observed using TRLFS (Bader et al, 2019). This technique also allowed identifying the association of phosphate species from this halophilic archaeon with Eu(III), the inactive analogue of Cm(III).…”

Section: X-ray Absorption Spectroscopy (Xas)mentioning

confidence: 99%

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

López-Fernández

Jroundi

Ruiz-Fresneda

et al. 2020

Microbial Biotechnology

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Radionuclides (RNs) generated by nuclear and civil industries are released in natural ecosystems and may have a hazardous impact on human health and the environment. RN-polluted environments harbour different microbial species that become highly tolerant of these elements through mechanisms including biosorption, biotransformation, biomineralization and intracellular accumulation. Such microbial-RN interaction processes hold biotechnological potential for the design of bioremediation strategies to deal with several contamination problems. This paper, with its multidisciplinary approach, provides a state-of-theart review of most research endeavours aimed to elucidate how microbes deal with radionuclides and how they tolerate ionizing radiations. In addition, the most recent findings related to new biotechnological applications of microbes in the bioremediation of radionuclides and in the long-term disposal of nuclear wastes are described and discussed.

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Section: X-ray Absorption Spectroscopy (Xas)mentioning

confidence: 99%

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

López-Fernández

Jroundi

Ruiz-Fresneda

et al. 2020

Microbial Biotechnology

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“…MT-2.99 and Pseudomonas fluorescens. , Recently, Yeasts and Archaea have also been investigated for their ability to complex An III (e.g., Cm) and trivalent lanthanides (Ln III ) (e.g., Eu) through carboxyl and phosphate groups. , In addition, biofilm formation by microorganisms has to be considered, as it can lead to the immobilization of bioabsorbed radionuclides within the DGR system and consequently can affect their integrity.…”

Section: Introductionmentioning

confidence: 99%

Molecular Binding of Eu^III/Cm^III by Stenotrophomonas bentonitica and Its Impact on the Safety of Future Geodisposal of Radioactive Waste

Ruiz-Fresneda

López-Fernández

Martinez-Moreno

et al. 2020

Environ. Sci. Technol.

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Microbial communities occurring in reference materials for artificial barriers (e.g., bentonites) in future deep geological repositories of radioactive waste can influence the migration behavior of radionuclides such as curium (CmIII). This study investigates the molecular interactions between CmIII and its inactive analogue europium (EuIII) with the indigenous bentonite bacterium Stenotrophomonas bentonitica at environmentally relevant concentrations. Potentiometric studies showed a remarkably high concentration of phosphates at the bacterial cell wall compared to other bacteria, revealing the great potential of S. bentonitica for metal binding. Infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the role of phosphates and carboxylate groups from the cell envelope in the bioassociation of EuIII. Additionally, time-resolved laser-induced fluorescence spectroscopy (TRLFS) identified phosphoryl and carboxyl groups from bacterial envelopes, among other released complexing agents, to be involved in the EuIII and CmIII coordination. The ability of this bacterium to form a biofilm at the surface of bentonites allows them to immobilize trivalent lanthanide and actinides in the environment.

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“…EDTA: ethylenediaminetetraacetic acid, LPS: lipopolysaccharide. References: (a) Moll et al 2014 , (b) Ozaki et al 2005 , (c) Ozaki et al 2006 , (d) Heller et al 2012 , (e) Barkleit et al 2013 , (f) Bader et al 2019 …”

Section: Resultsunclassified

“…The Eu(III) coordination environment in the cytosol showed similarities with (a) Eu(III) bound to the bacterial phosphate groups of the cell envelope of Sporomusa sp. and bacterial lipopolysaccharide (Moll et al 2014 ; Bader et al 2019 ), (b) Eu(III) complexed by the strong chelate-ligand EDTA, and (c) Eu(III) complexed by carboxyl groups of salicylic acid (Barkleit et al 2013 ). In this first approximation, we confirmed the interaction of Eu(III) with organic phosphate and carboxyl groups in a chelate-manner in the cytosol at the level of [Eu(III)] 0 200 μM.…”

Section: Resultsmentioning

confidence: 99%

Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure

Moll

Sachs

Geipel

2020

Environ Sci Pollut Res

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Experiments conducted over a period of 6 weeks using Brassica napus callus cells grown in vitro under Eu(III) or U(VI) stress showed that B. napus cells were able to bioassociate both potentially toxic metals (PTM), 628 nmol Eu/gfresh cells and 995 nmol U/gfresh cells. Most of the Eu(III) and U(VI) was found to be enriched in the cell wall fraction. Under high metal stress (200 μM), cells responded with reduced cell viability and growth. Subsequent speciation analyses using both metals as luminescence probes confirmed that B. napus callus cells provided multiple-binding environments for Eu(III) and U(VI). Moreover, two different inner-sphere Eu3+ species could be distinguished. For U(VI), a dominant binding by organic and/or inorganic phosphate groups of the plant biomass can be concluded.

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Association of Eu(III) and Cm(III) onto an extremely halophilic archaeon

Cited by 13 publications

References 44 publications

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

Molecular Binding of Eu^III/Cm^III by Stenotrophomonas bentonitica and Its Impact on the Safety of Future Geodisposal of Radioactive Waste

Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure

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Association of Eu(III) and Cm(III) onto an extremely halophilic archaeon

Cited by 13 publications

References 44 publications

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

Molecular Binding of EuIII/CmIII by Stenotrophomonas bentonitica and Its Impact on the Safety of Future Geodisposal of Radioactive Waste

Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure

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Molecular Binding of Eu^III/Cm^III by Stenotrophomonas bentonitica and Its Impact on the Safety of Future Geodisposal of Radioactive Waste