Association of CYP19A1 gene polymorphisms with reproductive traits in pigs

Zhou, Rong; Yang, Yalan; Liu, Ying; QiMei, Chen; Chen, Jie; Li, Kui

doi:10.1016/s2095-3119(16)61520-4

Cited by 6 publications

(6 citation statements)

References 39 publications

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“…BALB/c mice were purchased from the Wuhan Center for Disease Control. Ten different tissues, including heart, liver, spleen, lung, kidney, stomach, back muscles, leg muscle, abdominal fat, and small intestine of three 6-week-old male mice were collected and treated as our previous report (Zhou, Yang et al 2017). We also took the leg muscles of postnatal 2 days, 2 weeks, 4 weeks, 6 weeks, and adult mice to analyze miR-483-3p gene expression pattern.…”

Section: Tissue Samples Preparation and Cell Culturementioning

confidence: 99%

Murine miR-483-3p promotes proliferation and suppresses differentiation of C2C12 cells by targeting SRF

Huang

Liu

et al. 2017

Preprint

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Section: Tissue Samples Preparation and Cell Culturementioning

confidence: 99%

Murine miR-483-3p promotes proliferation and suppresses differentiation of C2C12 cells by targeting SRF

Huang

Liu

et al. 2017

Preprint

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“…Myogenesis is a complicated process regulated by a number of transcription factors, followed by a failure to undergo hypertrophic growth after birth (Li, Czubryt et al 2005 abdominal fat, and small intestine of three 6-week-old male mice were collected and treated 131 as our previous report (Zhou, Yang et al 2017). We also took the leg muscles of postnatal 2 …”

mentioning

confidence: 99%

Murine miR-483-3p promotes proliferation and suppresses differentiation of C2C12 cells by targeting SRF

Huang

Liu

et al. 2017

Preprint

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“…The expression of CYP17A1 was recognized as a feature of cells similar to those in the uterus (Kiezun et al, ). CYP19A1 was also associated with the proliferation capability of granulosa cells in vitro (Ciesiolka et al, ) and was highly expressed in the ovaries, spleen and uterus and lowly expressed in other tissues (Zhou et al, ). Therefore, the steroidogenesis and hormone receptor genes related to E2 and P4 and are very important for granulosa cells.…”

Section: Discussionmentioning

confidence: 99%

The effects of phospholipase C on oestradiol and progesterone secretion in porcine granulosa cells cultured in vitro

Chen

Yang

Wang

et al. 2019

Reprod Domestic Animals

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Granulosa cells play important roles in the regulation of ovarian functions. Phospholipase C is crucial in several signalling pathways and could participate in the molecular mechanisms of cell proliferation, differentiation and ageing. The objective of this study was to identify the effects of phospholipase C on the steroidogenesis of oestradiol and progesterone in porcine granulosa cells cultured in vitro. Inhibitor U73122 or activator m‐3M3FBS of phospholipase C was added to the in vitro medium of porcine granulosa cells, respectively. The secretion of oestradiol decreased after 2 hr, 8 hr, 12 hr, 24 hr and 48 hr of treatment with 500 nM U73122 (p < .05) and decreased after 2 hr of treatment in the 500 nM m‐3M3FBS addition group (p < .05). The secretion of progesterone increased after 4 hr of treatment with 500 nM U73122 (p < .05) and increased after 2 hr and 8 hr of treatment in the 500 nM m‐3M3FBS addition group (p < .05). The ratio of oestradiol to progesterone decreased at each time point, except 8 hr after the addition of 500 nM U73122 (p < .05). The ratio of oestradiol to progesterone decreased after 2 hr (p < .05) of treatment with 500 nM m‐3M3FBS. In genes that regulate the synthesis of oestradiol or progesterone, the mRNA expression of CYP11A1 was markedly increased (p < .05), and the mRNA expression of other genes did not change significantly in the U73122 treatment group, while the addition of m‐3M3FBS did not change those genes significantly despite the contrary trend. Our results demonstrated that phospholipase C can be a potential target to stimulate the secretion of oestradiol and suppress progesterone secretion in porcine granulosa cells cultured in vitro, which shed light on a novel biological function of phospholipase C in porcine granulosa cells.

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Association of CYP19A1 gene polymorphisms with reproductive traits in pigs

Cited by 6 publications

References 39 publications

Murine miR-483-3p promotes proliferation and suppresses differentiation of C2C12 cells by targeting SRF

Murine miR-483-3p promotes proliferation and suppresses differentiation of C2C12 cells by targeting SRF

Murine miR-483-3p promotes proliferation and suppresses differentiation of C2C12 cells by targeting SRF

The effects of phospholipase C on oestradiol and progesterone secretion in porcine granulosa cells cultured in vitro

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