Murine miR-483-3p promotes proliferation and suppresses differentiation of C2C12 cells by targeting SRF

Huang, Jing; Liu, Ying; Fu, Liangliang; Li, Hegang; Xie, Bingyan; Ma, Guojian

doi:10.7287/peerj.preprints.3454v1

Cited by 1 publication

(2 citation statements)

References 1 publication

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zhang et al, 2013). In C2C12 (mouse myoblasts) cells, miR-483 promoted the proliferation and inhibited the differentiation via targeting SRF (Huang et al, 2017). However, in the current study, miR-483 suppressed both the proliferation and the differentiation of bovine myoblast cells by targeting IGF1.…”

Section: Discussioncontrasting

confidence: 70%

“…The function of miR‐483 has been demonstrated in a variety of biological processes (Qiao et al, ; Song et al, ; Xiang et al, ; Z. Zhang et al, ). In C2C12 (mouse myoblasts) cells, miR‐483 promoted the proliferation and inhibited the differentiation via targeting SRF (Huang et al, ). However, in the current study, miR‐483 suppressed both the proliferation and the differentiation of bovine myoblast cells by targeting IGF1 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

miR‐483 inhibits bovine myoblast cell proliferation and differentiation via IGF1/PI3K/AKT signal pathway

Song

Dong

et al. 2018

Journal Cellular Physiology

View full text Add to dashboard Cite

MicroRNAs (miRNAs) have been established to regulate skeletal muscle development in mammals. However, few studies have been conducted on the regulation of proliferation and differentiation of bovine myoblast cells by miRNAs. The aim of our study was to explore the function of miR‐483 in cell proliferation and differentiation of bovine myoblast. Here, we found that miR‐483 declined in both proliferation and differentiation stages of bovine myoblast cells. During the proliferation phase, the overexpression of miR‐483 downregulated the cell cycle–associated genes cyclin‐dependent kinase 2 (CDK2), proliferating cell nuclear antigen (PCNA) messenger RNA (mRNA), and the protein levels. At the cellular level, cell cycle, cell counting kit‐8, and 5‐ethynyl‐2´‐deoxyuridine results indicated that the overexpression of miR‐483 block cell proliferation. During differentiation, the overexpression of miR‐483 led to a decrease in the levels of the myogenic marker genes MyoD1 and MyoG mRNA and protein. Furthermore, the immunofluorescence analysis results showed that the number of MyHC‐positive myotubes was reduced. In contrast, the opposite experimental results were obtained concerning both proliferation and differentiation after the inhibition of miR‐483. Mechanistically, we demonstrated that miR‐483 target insulin‐like growth factor 1 (IGF1) and downregulated the expression of key proteins in the PI3K/AKT signaling pathway. Altogether, our findings indicate that miR‐483 acts as a negative regulator of bovine myoblast cell proliferation and differentiation.

show abstract

Section: Discussioncontrasting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

miR‐483 inhibits bovine myoblast cell proliferation and differentiation via IGF1/PI3K/AKT signal pathway

Song

Dong

et al. 2018

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

Murine miR-483-3p promotes proliferation and suppresses differentiation of C2C12 cells by targeting SRF

Abstract: 31Myogenesis is a complicated process, which is regulated by numerous regulators.

Cited by 1 publication

References 1 publication

miR‐483 inhibits bovine myoblast cell proliferation and differentiation via IGF1/PI3K/AKT signal pathway

miR‐483 inhibits bovine myoblast cell proliferation and differentiation via IGF1/PI3K/AKT signal pathway

Contact Info

Product

Resources

About