Association of culture medium with growth, weight and cardiovascular development of IVF children at the age of 9 years

Zandstra, Heleen; Brentjens, L B P M Stevens; Spauwen, B.; Touwslager, Robbert N.H.; Bons, J; Mulder, A. L. M.; Smits, Luc; Hoeven, M.A.H.B.M. van der; Golde, Ron J T van; Evers, Johannes L.H.; Dumoulin, J.C.M.; Montfoort, Aafke P.A. van

doi:10.1093/humrep/dey246

Cited by 51 publications

(22 citation statements)

References 53 publications

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“…Subsequent prospective studies from the Dutch group with random allocation to two culture media groups showed a similar BW of the infants and altered growth patterns of the fetus from the second trimester of pregnancy and at 2 years of age between the different culture media (Dumoulin et al 2010;Nelissen et al 2012;Kleijkers et al 2014). A recent Dutch study observed that two culture media were associated with differences in body weight, BMI, truncal adiposity, waist circumference and waist/hip ratio in 9-year-old children conceived after ART, while no significant differences were observed regarding cardiovascular development (Zandstra et al 2018). Two other studies showed culture medium-dependent differences in mRNA expression levels of genes involved in apoptosis, protein degradation, metabolism and cell-cycle regulation (Kleijkers et al 2015a) and that the age of the embryo culture media was inversely associated with the BW of singletons born after ART (Kleijkers et al 2015b).…”

Section: Culture Mediamentioning

confidence: 82%

The health of children conceived by ART: ‘the chicken or the egg?’

Berntsen

Söderström‐Anttila

Wennerholm

et al. 2019

Human Reproduction Update

294

226

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Long-term health Neurodevelopmental health Cardiovascular function and metabolism Growth Respiratory and allergic disorders Malignancies Reproductive health Controlled ovarian stimulation Laboratory factors Culture media Culture time Number of embryos transferred Oocyte donation and gestational surrogacy Oocyte vitrification Epigenetics Imprinting disorders The chicken or the egg? Subfertility and sibling studies Future research Conclusion The conclusion we can reach from the well designed studies aimed at disentangling the influence on child health of parental and ART factors is that both the chicken and the egg matter.

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Section: Culture Mediamentioning

confidence: 82%

The health of children conceived by ART: ‘the chicken or the egg?’

Berntsen

Söderström‐Anttila

Wennerholm

et al. 2019

Human Reproduction Update

294

226

View full text Add to dashboard Cite

show abstract

“…On the other hand, the trophectoderm, whose genomic imprints are unstable and vulnerable to the surrounding microenvironment during implantation, appears to be sensitive to in vitro culture conditions and prone to an imbalance in imprinted gene expression [15,16]. Although the composition of the culture medium has continuously been improved, it is still not completely equivalent to the physiological conditions of the natural environment in vivo [17]. In addition, even with careful and gentle manipulation, the trophectoderm must withstand severe stress and interference from the environment, such as changes in temperature, pH, and oxygen tension; light exposure; pH variations during manipulation; and shear stress linked to repeated pipetting, which may interact and synergistically affect placental function and development [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

The placental transcriptome of the first-trimester placenta is affected by in vitro fertilization and embryo transfer

Zhao¹,

Zheng²,

Liu³

et al. 2019

Reprod Biol Endocrinol

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Background: The placenta is a highly specialized temporary organ that is related to fetal development and pregnancy outcomes, and epidemiological data demonstrate an increased risk of placental abnormality after in vitro fertilization and embryo transfer (IVF-ET). Methods: This study examines alterations in the transcriptome profile of first-trimester placentas from IVF-ET pregnancies and analyzes the potential mechanisms that play a role in the adverse perinatal outcomes associated with IVF-ET procedures. Four human placental villi from first-trimester samples were obtained through fetal bud aspiration from patients subjected to IVF-ET due to oviductal factors. An additional four control human placental villi were derived from a group of subjects who spontaneously conceived a twin pregnancy. We analyzed their transcriptomes by microarray. Then, RT-qPCR and immunohistochemistry were utilized to analyze several dysregulated genes to validate the microarray results. Biological functions and pathways were analyzed with bioinformatics tools. Results: A total of 3405 differentially regulated genes were identified as significantly dysregulated (> 2-fold change; P < 0.05) in the IVF-ET placenta in the first trimester: 1910 upregulated and 1495 downregulated genes. Functional enrichment analysis of the differentially regulated genes demonstrated that the genes were involved in more than 50 biological processes and pathways that have been shown to play important roles in the first trimester in vivo. These pathways can be clustered into coagulation cascades, immune response, transmembrane signaling, metabolism, cell cycle, stress control, invasion and vascularization. Nearly the same number of up-and downregulated genes participate in the same biological processes related to placental development and maintenance. Procedures utilized in IVF-ET altered the expression of first-trimester placental genes that are critical to these biological processes and triggered a compensatory mechanism during early implantation in vivo. Conclusion: These data provide a potential basis for further analysis of the higher frequency of adverse perinatal outcomes following IVF-ET, with the ultimate goal of developing safer IVF-ET protocols.

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“…insulin), amino acids, pyruvate, lactate and growth factors can induce alterations in birth weight, body growth rate and cardiovascular function (Banrezes et al 2011, Kannampuzha-Francis et al 2015. A similar situation has been found in humans, where the culture medium composition induced changes in birth weight (Kleijkers et al 2016) and body weight and BMI examined at 9 years of age (Zandstra et al 2018). Importantly, animal models have revealed that culture T39 M A Velazquez et al…”

Section: Assisted Reproductive Technologiesmentioning

confidence: 66%

Periconceptional environment and the developmental origins of disease

Velazquez

Fleming

Watkins

2019

Journal of Endocrinology

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The concept emerging from Professor David Barker’s seminal research on the developmental origins of later-life disease has progressed in many directions since it was first published. One critical question being when during gestation might environment alter the developmental programme with such enduring consequences. Here, we review the growing consensus from clinical and animal research that the period around conception, embracing gamete maturation and early embryogenesis might be the most vulnerable period. We focus on four types of environmental exposure shown to modify periconceptional reproduction and offspring development and health: maternal overnutrition and obesity; maternal undernutrition; paternal diet and health; and assisted reproductive technology. These conditions may act through diverse epigenetic, cellular and physiological mechanisms to alter gene expression and cellular signalling and function in the conceptus affecting offspring growth and metabolism leading to increased risk for cardiometabolic and neurological disease in later life.

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Association of culture medium with growth, weight and cardiovascular development of IVF children at the age of 9 years

Abstract: NTR4220.

Cited by 51 publications

References 53 publications

The health of children conceived by ART: ‘the chicken or the egg?’

The health of children conceived by ART: ‘the chicken or the egg?’

The placental transcriptome of the first-trimester placenta is affected by in vitro fertilization and embryo transfer

Periconceptional environment and the developmental origins of disease

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