Association of Coronary Artery Calcification with MDA-LDL-C/LDL-C and Urinary 8-Isoprostane in Japanese Patients with Type 2 Diabetes

Abstract

Objective Oxidative stress has been implicated in the development of coronary artery calcification (CAC). However, there are few reports on this issue in Japanese patients with diabetes. In this study, we examined the association of the CAC score (CACS) with oxidative stress markers. Methods The study subjects were 163 Japanese patients with type 2 diabetes (75 men and 88 women). The CACS (Agatston unit: AU) was measured by multi-detector computed tomography (MDCT), and the oxidative stress markers, such as the urinary 8-isoprostane and 8-hydroxydeoxyguanosine (8-OHdG) and serum malondialdehyde (MDA)-LDL cholesterol were measured. The relationships between CACS and oxidative stress markers were statistically analyzed. Results Compared with the CACS 0-400 AU group (n=132), the age, duration of diabetes, urinary 8-isoprostane levels, serum MDA-LDL-C/LDL-C and maximum intima media thickness (IMT) were higher, and body mass index and HbA1c level were lower, in the CACS >400 AU group (n=31). The multiple logistic regression analysis showed that a CAC >400 AU was independently associated with the urinary 8-isoprostane (>median) (OR=2.54, 95% CI=1.03-6.32, p=0.044), MDA-LDL-C/LDL-C (>median) (OR=2.62, 95% CI=1.07-6.40, p=0.035) and HbA1c (>median) (OR=0.32, CI=0.12-0.87, p<0.025). Focusing on oxidative stress, a higher MDA-LDL-C/LDL-C (p=0.026) and a higher urinary 8-isoprostane level (p=0.074) were associated with the CACS. Conclusion The CACS was found to be independently associated with the MDA-LDL-C/LDL-C and urinary 8-isoprostane levels in Japanese patients with type 2 diabetes.

Association of Advanced Glycation End Products with coronary Artery Calcification in Japanese Subjects with Type 2 Diabetes as Assessed by Skin Autofluorescence

“…IMT of the carotid arteries was measured using ultrasound diagnostic equipment (LOGIQ 500, GE Yokogawa Medical Systems Corp., Hino, Tokyo, Japan) with an electrical linear transducer (mid-frequency of 7.5 MHz). The common carotid artery, carotid bulb, and portions of the internal and external carotid arteries on both sides were scanned with the subject in the supine position 6) . We defined the max IMT as the thickest portion detected in the scanned regions.…”

“…CACS measurement has also been regarded as being clinically useful for evaluating coronary atherosclerosis in Japan 5) where the incidence of coronary heart disease is much lower than in Western countries. In a previous study, we showed CACS to be positively associated with biological markers of oxidative stress 6) and to predict the morbidity of CVD in Japanese subjects with type 2 diabetes 7) .…”

Involvement of oxidative stress in atherosclerosis development in subjects with sarcopenic obesity

“…Oxidative stress markers, such as urinary 8‐isoprostane and 8‐hydroxydeoxyguanosine (8‐OHdG) and serum malondialdehyde‐LDL cholesterol (MDA‐LDL‐C), were measured by SRL, Inc. (Tokyo, Japan). Hepatic steatosis was defined as a liver to spleen density ratio below 0.9, based on plain abdominal CT .…”

Circulating Malondialdehyde-Modified LDL-Related Variables and Coronary Artery Stenosis in Asymptomatic Patients with Type 2 Diabetes

“…Serum MDA-LDL levels have been positively correlated with carotid intima-media thickness [ 9 , 12 ]. Moreover, it was shown that the MDA-LDL-to-LDL-cholesterol (LDL-C) ratio (MDA-LDL/LDL-C) was a more useful predictor of coronary artery calcification than the MDA-LDL level alone [ 11 , 13 ]. Kurobe et al revealed that the vascular protective effects of ezetimibe, an inhibitor of cholesterol intestinal absorption, were correlated with decreased values of MDA-LDL and MDA-LDL/LDL-C but not with those of LDL-C [ 14 ].…”