Association of CD44 Isoform Immunohistochemical Expression with Myometrial and Vascular Invasion in Endometrioid Endometrial Carcinoma

Stokes, Gary N.; Shelton, Jack B.; Zahn, Christopher M.; Kendall, Brian

doi:10.1006/gyno.2001.6470

Cited by 10 publications

(9 citation statements)

References 10 publications

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“…It would, however, run contrary to the hypothesis that increased vCD44 expression occurs in more advanced, aggressive tumors. Stokes et al [32] found standard CD44 (sCD44) expression and depth of invasion to be inversely, but not significantly correlated, which is similar to our findings. On the other hand, Leblanc et al [28] reported CD44 expression to increase with depth of myometrial invasion.…”

Section: Discussionsupporting

confidence: 91%

“…Such a result is consistent with a hypothesis that highly invasive cancers tend to lose the standard variant. A similar but also insignificant relationship of CD44v6 expression was found by Ayhan et al and Stokes et al [26, 32], who found that CD44v6 expression strongly correlated with lack of myometrial invasion. Ayhan and Stokes suggest this could be a marker for cancers with myometrial involvement, which would thus facilitate preoperative qualification for lymphadenectomy.…”

Section: Discussionsupporting

confidence: 82%

“…On the other hand, loss of CD44v6 correlates with worse prognosis in atypical carcinoids, non-planoepithelial lung cancer, neuroblastoma, and bladder and prostate cancers [18, 20, 21, 39]. This reflects both the complexity of CD44 regulation in cancers, and its tissue specificity [18, 19, 32]. …”

Section: Discussionmentioning

confidence: 99%

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CD44 expression in curettage and postoperative specimens of endometrial cancer

Wojciechowski

Krawczyk

Śmigielski

et al. 2014

Arch Gynecol Obstet

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BackgroundAdhesive molecules like CD44 are well defined key players in the metastatic cascade in many cancers, including endometrial cancer. They could play a role of markers of invasion, metastasis and prognostic factors.Aim of the studyThe aim of the study is to assess a possible role of the CD44 as a marker of invasion in endometrial cancer, both at the moment of preoperative workup and final staging.Materials and methodsAvailable for analysis were archival specimens of 51 patients who had underwent curettage and surgery between 2002 and 2007. An immunohistochemical study for CD44 expression was performed in curettage and postoperative specimens. Normal endometrium of 20 randomly chosen patients was used as a control group.ResultsIn endometrial cancer the expression of CD44 was significantly more intensive than in normal endometrium. In postoperative specimens, the CD44 expression was weaker in serous than in endometrioid cancer. There was no significant correlation between the adhesion molecule expression and clinicopathological features: grade,depth of invasion, cervical involvement, serosal and adnexal involvement, lymph-vascular space involvement, lymph node and distant metastases nor FIGO stage.ConclusionsAn increased expression of CD44 in endometrial cancer suggests its possible role in pathogenesis of this disease, however, it doesn’t seem to be crucial. Different expression of the CD44 in endometrioid and papillary-serous type may reflect different pathogenesis of these types of cancer. No statistically proved relation between the investigated molecule expression and clinicopathological parameters suggests scepticism about its use in diagnostic process of endometrial cancer.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 82%

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CD44 expression in curettage and postoperative specimens of endometrial cancer

Wojciechowski

Krawczyk

Śmigielski

et al. 2014

Arch Gynecol Obstet

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“…The living rate of lung cancer patients with positive expression of CD44 was obviously lower than that of negative expression group [17] . Some found that high expression of CD44v existed in the perpherel blood and bone marrow in patients with micrometastasis of gastric cancer [18][19][20][21] . The expression of CD44s could also be used as markers for some benign and malignant tumors [21][22][23] .…”

Section: Discussionmentioning

confidence: 98%

Expression of CD44s mRNA in gastric carcinoma

Ai-hua

Wang²,

Guo

et al. 2007

Chin. J. Cancer Res.

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Objective:To study the expression of CD44s mRNA in the occurrence, development and invasion of gastric carcinoma (GC). Methods: The expressions of CD44s mRNA in 66 cases of GC, 25 cases of superficial gastritis and 25 cases of atypical hyperplasia were examined by in situ hybridization (ISH). Results: There was no expression of CD44s mRNA in the group of superficial gastritis; the positive rate was 20%(5/25) in the group of atypical hyperplasia and 62.12%(41/66) in the group of gastric carcinoma. The positive rate in poor differentiation group was significantly higher than that in well differentiation group (P<0.05), and the positive rate of lymph node metastasis group was significantly higher than that in negative lymph node metastasis group(P<0.05). Conclusion: The expression of CD44s mRNA was related to cell differentiation degree and lymph node metastasis, the activation of CD44s gene was related to strong invasion of cancer cells and poor prognosis.Gastric carcinoma (GC) is one of the mo9st common malignant tumors. CG is in the first place of death among all malignant tumors worldwide [1,2] . In recent years, researches on the molecular mechanism of oncogenesis and metastasis and the correlated markers of GC are important objects in clinical research in oncology [2][3][4] . In order to investigate the relationship between the over expression of CD44s mRNA and the oncogenesis invasion, metastasis, and pathological grade of GC, 66 specimens of GC and 50 specimens of non gastric tissue were examined by in situ hybridization technology. MATERIALS AND METHODS Clinical DataThe specimens of GC gastrectomy from 66 patients (49 males and 17 females) with an average age of 57.91 ⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯ years, median age of 59 years (range from 37-79 years)were collected from the department of tumor surgery, College of the First Clinical Medicine, Hebei North University. Specimens from 25 cases of superficial gastritis and 25 cases of gastritis with atypical hyperplasia were also collected among them there were 38 males and 12 females with an average age of 50 years and median age of 48.5 years(range from 39-65 years). Specimens ProcessingGastrectomy specimens from surgery were fixed immediately for 4 hours with 4% formaldehyde in 0.1 mol/L PBS (pH 7.2 containing 0.1% DEPC), then dehydrated, embedded in paraffin wax, sliced up and stained with haematoxylin and eosin. Cases were diagnosed by two seasoned professor. TNM criteria of WHO was applied to the histopathological classification, lymph node metastasis was observed. The multi tissue sections (7 μm) for in situ hybridization were besmeared to the slices with ploy-lysine and kept at 37℃ for use. ReagentsThe kit of in situ hybridization was bought from Boster Biological Technology Ltd.

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“…CD44 is a transmembrane receptor protein that belongs to the family of adhesion molecules and has a critical role in extracellular matrix adhesion and is implicated in a series of cellular events, such as lymphocyte homing, leukocyte activation, lymphopoiesis, embryogenesis, and wound healing 19 . With regard to CD44 and its variants, several studies have investigated its expressions in endometrial pathologies, including adenocarcinomas [20][21][22][23][24][25] . In our study we observed higher methylation in CD44 gene, but with no statistical significance.…”

Section: Discussionmentioning

confidence: 99%

Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA

Dvořáková¹,

Chmelařová²,

Laco³

et al. 2013

BIOMED PAP

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Background. Epigenetic changes are considered to be a frequent event during tumor development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumor suppressor genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in endometrial cancer by comparison with normal endometrial tissue. Materials and Methods. We used MS-MLPA (Methylation-specific Multiplex ligation-dependent probe amplification) to compare the methylation status of 59 tissue samples of endometroid type of endometrial carcinoma with 20 control samples of non-neoplastic endometrium. Results. Using 15% cut-off for methylation, we observed significantly higher methylation in the CDH13 gene in endometrial cancer group. We observed significantly higher methylation in both WT1 and GATA5 genes in IB stage of endometroid carcinoma. We also observed significantly higher methylation in GATA5 gene in the group of poorly differentiated endometroid carcinoma. Conclusion. The findings suggest the importance of hypermethylation of CDH13, WT1 and GATA5 genes in endometrial carcinogenesis and could have implications for future diagnostic and therapeutic strategies of endometrial cancer based on epigenetic changes.

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Association of CD44 Isoform Immunohistochemical Expression with Myometrial and Vascular Invasion in Endometrioid Endometrial Carcinoma

Cited by 10 publications

References 10 publications

CD44 expression in curettage and postoperative specimens of endometrial cancer

CD44 expression in curettage and postoperative specimens of endometrial cancer

Expression of CD44s mRNA in gastric carcinoma

Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA

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