Association of CCND1 Gene c.870G>A Polymorphism with Breast Cancer Risk: A Case-ControlStudy and a Meta-Analysis

Abstract

Cyclin D1 (CCND1) plays an essential role in regulating the progress of the cell cycle from G1 to S phase. There is a common c.870G>A polymorphism in the CCND1 gene. The aim of this study was to investigate the association of CCND1 gene c.870G>A polymorphism with breast cancer risk in a case-control study, which followed by a meta-analysis and an in silico analysis. Three hundred and thirty-five subjects composed of 174 women with breast cancer and 161 healthy controls were included in the case-control study. CCND1 gene c.870G>A genotyping was performed by PCR-RFLP. Meta-analysis was done for 14 studies composed of 7281 cases and 6820 controls. Some bioinformatics tools were applied to investigate the effects of c.870G>A on the mRNA splicing and structure. Our data obtained from case-control study revealed that GA genotype (OR: 1.89, 95%CI: 1.12-3.17, p = 0.017), AA genotype (OR: 1.95, 95%CI: 1.08-3.53, p = 0.027), and A allele (OR: 1.44, 95%CI: 1.06-1.95, p = 0.019) were significantly associated with breast cancer risk. The results of meta-analysis showed a significant association between CCND1 c.870G>A polymorphism and breast cancer risk, especially in Caucasian population. In silico analysis revealed that c.870G>A transition affect CCND1 mRNA splicing and secondary structure.

IL-1RA VNTR and IL-1α 4845G>T polymorphisms and risk of idiopathic male infertility in Iranian men: A case-control study and an in silico analysis

“…Therefore, in this study we used bioinformatics software for analysing the molecular effects of IL‐1RA VNTR, and IL‐1α 4845G>T polymorphisms. Our data revealed that IL‐1RA VNTR alters RNA splicing pattern; furthermore, this issue could influence the gene expression (McManus & Graveley, ; Soleimani et al, ). According to Korthagen et al, (), IL‐1RA VNTR could affect the gene expression which can explain the role of this polymorphism in male infertility resulting from altered levels of IL‐1RA in the testis.…”

Association between Cyclin D1 G870A (rs9344) polymorphism and cancer risk in Indian population: meta-analysis and trial sequential analysis

“…These findings are further strengthened by another meta-analyses conducted by Lu et al (2009) [ 59 ] and Cui et al (2012) [ 60 ] that showed the association of AA genotype of CCND1 G870A polymorphism with breast cancer susceptibility. Similarly, Soleimani et al (2016) [ 61 ] showed a significant association between CCND1 G870A polymorphism and breast cancer risk but in Caucasians. A meta-analysis conducted Wen et al (2014) [ 62 ] supported our data that CCND1 G870A polymorphism is a potential risk factor in the development of esophageal cancer.…”

Data mining analysis of miR-638 and key genes interaction in cisplatin resistant triple-negative breast cancer

“…Genetic alterations study with cBioportal revealed al terations of CCND1 in 35% of patient samples, with am plification as the highest alterations. Previous studies demonstrated that amplification in CCND1 is an early event in the development of a breast cancer stem cells (Burandt et al 2016), and mutations in CCND1 is associ ated with increased risk of breast cancer (Soleimani et al 2017). A previous study demonstrated that overexpression of CCND1 has occurred through amplification, transloca tion, or posttranscriptional regulation (Xu and Lin 2018).…”