Association of ALOX5APgene single nucleotide polymorphisms and cerebral infarction in the Han population of northern China

Zhang, Shuang-yan; Xu, Min; Zhang, Cuie; Qu, Zhengyi; Zhang, Binbin; Zheng, Zu-yan; Li-ming, Zhang

doi:10.1186/1471-2350-13-61

Cited by 16 publications

(5 citation statements)

References 19 publications

(30 reference statements)

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“…The risk of CI may vary according to the presence of various genetic and environmental factors. In addition, the relationship between the functional variation of multiple gene in different ethnic groups and CI risk has been studied, including the polymorphisms of interleukin-6 gene [ 2 ], β-fibrinogen gene [ 3 ], PON1 hypermethylation and PON3 hypomethylation [ 4 ], 5-lipoxygenase-activating protein gene association (ALOX5AP) [ 5 ], the cytochrome P450 1A1 (CYP1A1) gene [ 6 ], the human scavenger receptor class B type I (SR-BI) gene [ 7 ], genes associated with lipid metabolism [ 8 , 9 ], and others [ 10 ]. In general, it is important for both risk prediction and prevention of CI by identifying genetic risk.…”

Section: Introductionmentioning

confidence: 99%

The SNPs rs429358 and rs7412 of APOE gene are association with cerebral infarction but not SNPs rs2306283 and rs4149056 of SLCO1B1 gene in southern Chinese Hakka population

Huang

et al. 2020

Lipids Health Dis

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Background: Apolipoprotein E (ApoE) and solute carrier organic anion transporter family member 1B1 (SLCO1B1) regulate lipid metabolism. However, the relationship between genetic polymorphisms of APOE and SLCO1B1 and cerebral infarction (CI) remains unclear. Methods: A total of 938 CI patients and 1028 control participants were included in the study. The rs429358 and rs7412 single nucleotide polymorphisms (SNPs) in the APOE gene and rs2306283 and rs4149056 SNPs in the SLCO1B1 gene were analyzed by fluorescence polymerase chain reaction (PCR).

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Section: Introductionmentioning

confidence: 99%

The SNPs rs429358 and rs7412 of APOE gene are association with cerebral infarction but not SNPs rs2306283 and rs4149056 of SLCO1B1 gene in southern Chinese Hakka population

Huang

et al. 2020

Lipids Health Dis

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show abstract

“…Another study carried out in a large Chinese cohort reported a significant association of SG13S114AA genotype with a 1.62 fold risk for thrombotic stroke. The other variants of ALOX5AP gene evaluated in the study did not associate with stroke or its subtypes [21]. Kaushal et al (2007) found rs957640 and rs769874 to be associated with stroke in Whites in the United State (US) [22].…”

Section: Discussionmentioning

confidence: 96%

Association of ALOX5AP1 SG13S114T/A variant with ischemic stroke, stroke subtypes and aspirin resistance

Sharma

Dadheech

Kaul

et al. 2013

Journal of the Neurological Sciences

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“…Unlike the results of other existing studies on myocardial infarction area [5,7], the results achieved by the authors revealed that the total number of differentially expressed lncRNAs in the border zone was more than that of the myocardial infarction area, demonstrating more occurrences of active and more complex biological processes. For instance, LncRNA: AY212271, showing a coexpression relationship to Alox5ap, might be involved in various in ammatory reactions [12][13][14][15][16]. EF424788 and MRAK088538 were co-expressed with Itgb2, and Itgb2 was reported to be a risk factor for myocardial infarction and atherosclerotic thrombotic cerebral infarction [17][18][19].…”

Section: Discussionmentioning

confidence: 99%

NR_027324 regulates autophagy and apoptosis by combining with miR-103-3p as ceRNA to regulate ATG5 in cardiomyocytes during hypoglycemic and hypoxic injury

Meng¹,

Tan²,

Gu³

et al. 2020

Preprint

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Background: Myocardial infarction (MI) refers to a fatal disease, and the border zone (BZ) of myocardial infarction is of high importance to the prognosis of myocardial infarction patients. Autophagy and apoptosis can significantly impact cardiovascular diseases. In the previous studies conducted by the authors, plenty of differential expressions of long non-coding RNA (lncRNA) were reported in the border zone of myocardial infarction. As revealed from the results of bioinformatics analysis, LncRNA may take up a vital part in the pathological process of cardiovascular disease by regulating apoptosis and autophagy. This study aimed to firstly conduct bioinformatics analyses to predict that NR_027324, as a competitive endogenous RNA (ceRNA), binds to miR-103-3p and subsequently regulates the expression of downstream target gene ATG5, to secondly verify the mentioned regulatory relationship by molecular biological experiments, and thirdly to investigate that the above pathways transmit apoptosis and autophagy signals in cardiomyocytes during hypoglycemic and anoxic injuries. Methods: The binding of NR_027324 to miR-103-3p was predicted by the bioinformatics analysis, and then the expression of downstream ATG5 was regulated. By the dual luciferase assay, the binding of NR_027324 to miR-103-3p was confirmed. H9c2 cells underwent the culture under low glucose and hypoxia to simulate cardiomyocytes under ischemia. NR_027324 siRNA and overexpression plasmid vector and miR-103-3p (mimics and inhibitors) were adopted to transfect cells to examine its function in cardiomyocytes. By the reverse transcription-quantitative polymerase chain reaction (RT-PCR), the expression levels of NR_027324, miR-103a-3p and ATG5 were assessed. Cell viability was measured by the MTT assay, and cell injury was analyzed by the lactate dehydrogenase assay (LDH). The western blotting assay was performed to detect the expressions of Bcl2, Bax, Atg5, cleaved-caspase3 and cleaved-caspase9. Moreover, the immunofluorescence assay was used to detect the expression of LC3-I/II as a marker of autophagy.Results: In hypoglycemic and anoxic H9C2 cells, the expressions of NR_027324 and ATG5 were up-regulated, while miR-103-3p showed a down-regulated expression. By the dual luciferase assay, the binding of NR_027324 to miR-103-3p was confirmed. The overexpressed NR_027324 could enhance the reduced cell viability of hypoglycemic and anoxic H9C2 cells and reduce cell damage. Interfering with the expression of NR_027324 could further reduce the viability of hypoglycemic anoxic H9C2 cells, increase injury, inhibit protective autophagy and accelerate apoptosis of hypoglycemic anoxic H9C2 cells. The mentioned pathophysiological processes were achieved by NR_027324 binding to miR-103-3p and then regulating the expression of target gene ATG5.Conclusion: In hypoglycemic and anoxic H9C2 cells, the expressions of NR_027324 and ATG5 are up-regulated, while the expression of miR-103-3p decreases. NR_027324 acts as ceRNA combined with miR-103-3p to regulate ATG5 and control autophagy and apoptosis in cardiomyocytes during hypoglycemic and anoxic injuries.

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Association of ALOX5APgene single nucleotide polymorphisms and cerebral infarction in the Han population of northern China

Cited by 16 publications

References 19 publications

The SNPs rs429358 and rs7412 of APOE gene are association with cerebral infarction but not SNPs rs2306283 and rs4149056 of SLCO1B1 gene in southern Chinese Hakka population

The SNPs rs429358 and rs7412 of APOE gene are association with cerebral infarction but not SNPs rs2306283 and rs4149056 of SLCO1B1 gene in southern Chinese Hakka population

Association of ALOX5AP1 SG13S114T/A variant with ischemic stroke, stroke subtypes and aspirin resistance

NR_027324 regulates autophagy and apoptosis by combining with miR-103-3p as ceRNA to regulate ATG5 in cardiomyocytes during hypoglycemic and hypoxic injury

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