Association of a SNP in KISS 1 gene with reproductive traits in goats

Sahoo, Siddhant Sekhar; Mishra, Chinmoy; Kaushik, Rakesh; Rout, P. K.; Singh, Manoj Kumar; Bhusan, Saket; Dige, Mahesh Shivanand

doi:10.1080/09291016.2019.1608730

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…Additionally, research has shown that kisspeptin plays a part in the formation of the placenta. 10 Because KISS1 is important as a regulator of puberty initiation, variations in this gene are anticipated to be associated with reproductive features in goats such as high prolificacy, sexual precocity, and year-round estrus phenotypes. Kisspeptins may have a role in photoperiodic reproductive regulation in hamsters.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism studies and candidate genes associated with litter size traits in Indonesian goats. a systematic review

et al. 2023

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Background: Litter size (LS) is a significant, challenging, and economical aspect of the goat industry in Indonesia. It is influenced by several different factors and genes; consequently, identifying potential genes and loci associated with litter size has become a genetic problem. Several genetic indicators have been found to be associated with litter size in goats. This has prompted the need to discuss candidate genes associated with litter size in goats in Indonesia. Methods: A systematic review was conducted using critical databases including ResearchGate, Google Scholar, PubMed, Google search engine and Science direct. There were any exclusion criteria, they were as follows: articles published in languages other than English, Conference papers, short communication papers and papers not related to animals. After reviewing the abstracts of 42 publications, the remaining 17 investigations were chosen for full paper evaluation. A further eight studies were removed after a comprehensive evaluation of the publications because they did not match our inclusion criteria. Results: These markers include growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), bone morphogenetic protein receptor type IB (BMPR1B), and kisspeptin (KISS1). Single nucleotide polymorphisms in these genes contribute to the development of novel genetic markers that helps in the selection of goats with the most favorable genotypes for litter size. This type of genetic selection is more successful than the traditional way of selecting animals for reproductive traits, particularly litter size. Conclusions: As a result, this study summarizes the genetic impacts of polymorphisms in candidate genes associated with litter size features in Indonesian goats.

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Section: Introductionmentioning

confidence: 99%

Polymorphism studies and candidate genes associated with litter size traits in Indonesian goats. a systematic review

et al. 2023

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“…In addition, when the intron region is used as an enhancer, the associated SNP variant affects the expression of genes and may affect obesity in humans [43]. In livestock, variation in the promoter zone may affect the number of litter births in sheep [44], breed differentiation, and fat deposition of pigs [45; 46]. Therefore, as an important regulatory region of gene expression, the promoter region can also change the gene expression level by affecting the binding e ciency of a variety of transcription factors, thereby affecting gene function polymorphisms and animal body development process.…”

Section: Discussionmentioning

confidence: 99%

A novel candidate gene CLN8 regulates fat deposition in avian

Zhang

Sun

et al. 2022

Preprint

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Background The fat deposition has a crucial role in animal meat flavor, and fat deposition-related traits are vital for breeding in the commercial duck industry. Avian fat-related traits are typical complex phenotypes, which need a large amount of data to analyze the genetic loci. Results In this study, we newly phenotyped fat traits and genotyped whole-genome variations for 1246 ducks, and combed with previous GWAS data to reach 1880 ducks for following analysis. The carcass composition traits, subcutaneous fat weight (SFW), subcutaneous fat percentage (SFP), Abdominal fat weight (AFW), Abdominal fat percentage (AFP) and the body weight of day 42 (BW42) for each duck were collected. We identified a set of new loci that affect the traits related to fat deposition in avian. Among these loci, Ceroid-lipofuscinosis, neuronal 8 (CLN8) is a novel candidate gene controlling fat deposition. We investigated its novel function and regulation in avian adipogenesis.Five significant SNPs and a single haplotype were detected in the upstream of gene CLN8 and the most significant SNP (P-value = 21.37E-12) for subcutaneous fat percentage was obtained. Subsequently, luciferase assay demonstrated that five linkage mutations in the upstream of the CLN8 gene significantly decreased the transcriptional activity of CLN8. Further, ATAC-seq analysis showed that transcription factor binding sites were identified in a region close to the haplotype. A set of luciferase reporter gene vectors that contained different deletion fragments of the CLN8 promoter were constructed, and the core promoter area of CLN8 was finally identified in the − 1884/-1207 bp region of the 5′ flanking sequences, which contains adipogenesis-related transcription factors binding sites. Moreover, the over-expression of CLN8 can remarkably facilitate adipocyte differentiation in ICPs. Consistent with these, the global transcriptome profiling and functional analysis of the over-expressed CLN8 in the cell line further revealed that the lipid biosynthetic process during the adipogenesis was significantly enriched. Conclusions Our results demonstrated that CLN8 is a positive regulator of avian adipocyte differentiation. These findings identify a novel function of CLN8 in adipocyte differentiation, which provides important clues for the further study of the mechanism of avian fat deposition.

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“…Therefore, the KiSS1 gene is considered to be an important candidate gene for reproductive traits in livestock and humans [3,25]. It has been suggested that the KiSS1 gene plays a crucial role in reproductive performance in animal species [6,26,27]. It was previously reported [20] that six KiSS1 gene polymorphisms were found in five goat breeds with different prolificacy.…”

Section: Discussionmentioning

confidence: 99%

Association of the KiSS1 gene with litter size in Cyprus and Iraqi black goats

Rahawy

Al-Mutar

2021

Vet World

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Aim: The study investigated the genetic polymorphism of the kisspeptin (KiSS1) gene and its relationship with litter size in Cyprus and Iraqi black goats. Materials and Methods: Blood samples (n=124) were collected from the two goat breeds reared at the Agricultural Research-Ruminant Research Station Breeding Station, Baghdad, Iraq. Genomic DNA was isolated using a DNA extraction kit. Polymerase chain reaction (PCR) was used to amplify the KiSS1 gene. All PCR products were sequenced and samples were used for further analysis using NCBI-Blast online on the exon 1 (595 bp) region of the KiSS1 gene. Results: The results of this study revealed a significantly (P<0.05) larger litter size of the Cyprus goat breed than in the Iraqi black goats in the first and second parity. Three (893G/C, 973C/A, and 979T/G) substitutions relative to the KiSS1 gene reference sequence (GenBank ID: J × 047312.1, KC989928.1) were identified. Only the mutation g893G>C was identified as a single nucleotide polymorphism (SNP) associated with litter size. Furthermore, the average alleles in KiSS1 gene of both types of goats 0.567 and 0.3715 GG, were recorded. The genotyping at locus g893C>G was demonstrating domination of fecundity quality litter size, Both genotypes SNP of GC were classified at this marked region of KiSS1 gene. Conclusion: The study concluded that the role of the KiSS1 gene in fecundity, revealing the status of this gene as an indicator in the assisted of caprine breeding selection.

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Association of a SNP in KISS 1 gene with reproductive traits in goats

Cited by 13 publications

References 25 publications

Polymorphism studies and candidate genes associated with litter size traits in Indonesian goats. a systematic review

Polymorphism studies and candidate genes associated with litter size traits in Indonesian goats. a systematic review

A novel candidate gene CLN8 regulates fat deposition in avian

Association of the KiSS1 gene with litter size in Cyprus and Iraqi black goats

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