Association between vitamin D receptor gene haplotypes and chronic periodontitis among Japanese men

Abstract

Background:The vitamin D receptor (VDR) is involved in a variety of biological processes, such as bone metabolism and modulation of the immune response. Recent findings suggest that the pathway involving bone mineral density-mediated effects is important for the development of periodontitis, but their effects of combined VDR gene polymorphisms have not been confirmed on periodontitis. We assessed the relationship between ApaI, BsmI, and FokI VDR polymorphisms and the risk of severe chronic periodontitis among Japanese adult men. Materials and Methods:In a cross-sectional study, we examined 97 unrelated healthy Japanese men (mean age: 45.6 years, range: 22-59). A clinical examination was performed at a worksite health checkup, and information was obtained using a self-reported questionnaire. DNA was extracted from whole blood, and the VDR ApaI, BsmI, and FokI polymorphisms were genotyped using polymerase chain reaction. Results: F-carriers of FokI VDR polymorphisms were less likely to develop severe chronic periodontitis than non-F-carriers (p = 0.09). The ApaI and BsmI VDR polymorphisms did not show significant differences in the alleles or genotypes between the subjects with or without severe chronic periodontitis. The haplotype analysis of the three combined VDR polymorphisms revealed that the Abf homozygote had a notably higher prevalence of severe chronic periodontitis than the others, and adjustments for age, smoking status, number of teeth present, and prevalence of diabetes did not change this association (OR = 7.5; 95% CI = 1.6-34.4; p = 0.01). Conclusion:The VDR haplotype constructed from the ApaI, BsmI, and FokI polymorphisms is related to the risk of severe chronic periodontitis in Japanese men.

The NRAMPI, VDR and TNF-α gene polymorphisms in Iranian tuberculosis patients: the study on host susceptibility

“…In this study, only TNF-α-308A allele was associated with pulmonary TB (Table 1). Similar to our finding, Bikmaeva et al suggested an association of -308A allele with risk of pulmonary TB [26]. Generally, G to A substitution at position -308 represents a functional polymorphism which leads to different transcription rates in TNF-α production.…”

“…Although, the polymorphisms in position,-238, and -308 have been extensively studied in tuberculosis [24,25]. Previous studies demonstrated no association of particular allele or genotype of TNF-α with TB in different Asians, i.e., Indian, Turkish and Thai population [6,16,26]. In this study, only TNF-α-308A allele was associated with pulmonary TB (Table 1).…”

Vitamin D: relevance in dental practice

“…Vice-versa, in combination with Fc-gammaRIIIB genotype, BsmI may be associated with generalized early-onset periodontitis or, as demonstrated by de Brito, when the allele B of BsmI polymorphism and the T allele of TaqI polymorphism were present, the TB haplotype seemed to increase susceptibility to chronic periodontitis (37)(38)(39). The findings of Gunes indicates that the ApaI, BsmI and TaqI polymorphisms analyzed individually, did not show statistically significant differences in their frequencies in a Turkish population of healthy subjects and patients affected by chronic periodontal disease while, also in this case, the presence of allele T (TaqI) and B (BsmI) in the haplotype BT is over-represented among chronic periodontal cases (40).…”

Vitamin D receptor (FokI, BsmI and TaqI) gene polymorphisms and type 2 diabetes mellitus : A North Indian study

“…[28] Some of the other polymorphisms in the VDR gene identiÞ ed by allelic variation in restriction enzyme sites are Tru9I, TaqI, EcoRV and ApaI. [29] All these are located between exons 8 and 9 except that FokI is in exon 2. FokI polymorphism has been shown to have functional role in trascriptional activation of VDR gene.…”