The goal of this study was to see if there was a link between LH, FSH, testosterone, oestradiol, progesterone, and metabolic markers, including AMH and vitamin D, in a woman.. PCOS patients had significant increases in serum levels of LH (P <0.01), FSH (P 0.05), LH/FSH ratio (P <0.05), testosterone (P <0.01), progesterone (P <0.05), antimalaria (P <0.001), glucose (P <0.01), total cholesterol (P 0.01), triglycerides (P <0.001), LDL (P <0.01), VLDL (P <0.05), and Vitamin D (P <0.01). AMH appeared to be favourably connected with higher levels of LH, FSH, LH/FSH ratio, testosterone, progesterone, hyperglycemia, total cholesterol, triglycerides, LDL, and VLDL, and uncorrelated with oestradiol and HDL. We can conclude that AMH can potentially be applicate as a diagnostic marker for PCOS, the results also showed vitamin D deficiency is related to pathophysiology of PCOS. Vitamin D serum levels showed an inverse correlation with hyperglycaemia, hyperlipidaemia, and hormonal disturbances in PCOS.