Association between urinary levels of 8-hydroxy-2-deoxyguanosine and F2a-isoprostane in male football players and healthy non-athlete controls with dietary inflammatory and antioxidant indices

Abstract

PurposeThe relationship between the inflammatory and antioxidant potential of an athlete's diet and their oxidative biomarkers is an important area of investigation. Therefore, this study aimed to assess the excretion of 8-hydroxy-2-deoxyguanosine (8-OHdG) and F2alpha-isoprostane (F2a-IP) in the urine of male football players and healthy non-athlete controls. This study also aimed to examine the associations among the dietary inflammatory index (DII), the dietary total antioxidant capacity (DTAC), and the dietary phytochemical index (PI) with 8-OHdG and F2a-IP.MethodsIn this descriptive-analytical study, 45 male football players and 45 healthy non-athletes, who were individually matched based on age and body mass index (BMI), were recruited from Shiraz City, Iran. Fasted urine samples were analyzed for 8-OHdG and F2a-IP levels. Anthropometric measurements were performed, and body composition was assessed using a body composition analyzer. A valid food frequency questionnaire (FFQ) was used to calculate DII, DTAC, and PI scores. Data analysis was conducted using a generalized estimating equation (GEE) model.ResultsWe found that 8-OHdG (β = −6.96), F2a-IP (β = −82.58), and DII (β = −2.06) were significantly lower, while DTAC (β = 2.37) and PI (β = 0.084) were significantly higher in the football player group compared with the non-athlete group (P < 0.001 for all variables). In all participants, dietary indices were significantly associated with oxidative biomarkers. DII was positively associated with 8-OHdG (β = 2.25; P < 0.001) and F2a-IP (β = 38.34; P < 0.001). Furthermore, negative associations between DTAC (β = −1.42; P < 0.001) and PI (β = −35.37; P < 0.001) with 8-OHdG were found. Moreover, DTAC (β = −17.34; P < 0.001) and PI (β = −428.11; P = 0.003) were negatively associated with F2a-IP.ConclusionThe results of this study highlighted the importance of a healthy diet in reducing oxidative stress among football athletes. The levels of urinary biomarkers for DNA and lipid oxidation were found to be lower in football players compared to non-athletes. This suggests that following an anti-inflammatory and antioxidant-rich diet may help reduce oxidative stress in these individuals.

Effect of a plant-based diet on oxidative stress biomarkers in male footballers

“…This descriptive-analytical study is part of a research project, some of the findings of which have already been published 27 , 28 . A total of 90 males (45 footballers and 45 healthy non-athlete controls) aged 20–30 with a BMI of 20–25 kg/m 2 from Shiraz, Iran, participated in the study.…”

The association between diet quality indices and oxidative stress biomarkers in male footballers and healthy active controls

“…The current research data were obtained from a research project, and some of the findings have been previously published [ 27 – 29 ]. The current descriptive-analytical study was conducted on 90 males in two groups (45 footballers and 45 active controls), and the groups were individually matched according to BMI and age.…”

Dietary Antioxidant Capacity Indices are Negatively Correlated to LDL-Oxidation in Adults

“…The total mean of TAC was considered for similar foods (for example, some types of breads). At the end, the frequency of each food item intake was multiplied by its corresponding FRAP value, then summed up to estimate participant's DTAC [ 29 , 30 ].…”

“…Potatoes were often consumed as a starchy food and were not considered as vegetables. Natural juices of vegetables and fruits were included in the vegetable and fruit groups, because these components are also considered as high sources of phytochemicals [ 29 , 30 ].…”