Association between the stages of cervical cancer and chromosome 1 aneusomy

Cortés‐Gutiérrez, Elva I.; Dávila-Rodríguez, Martha I.; Muraira-Rodríguez, Marycarmen; Said‐Fernández, Salvador; Cerda‐Flores, Ricardo M.

doi:10.1016/j.cancergencyto.2004.10.001

Cited by 15 publications

(11 citation statements)

References 17 publications

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“…Given the high number of DNA breakages found in HG-SIL and the low number of DNA breakages detected in patients with LG-SIL, we suggest that the progression to malignant transformation involves an increase in the instability in chromosome-1. These results are in accordance with those of previous studies, which showed a significant positive correlation between numerical and structural aberrations in chromosome-1 and CIN lesions [5,20–23]. …”

Section: Discussionsupporting

confidence: 93%

“…Structural rearrangements of chromosome-1 (e.g., gains, deletions, translocations, and isochromosomes) have been described as the most frequent karyotypic changes in this type of tumor; for example, >95% of patients show rearrangements in this chromosome [2,3]. Aneusomy of chromosome-1 is strongly associated with infection high-risk HPV, and high-grade squamous intraepithelial lesions (HG-SIL) [4,5]. …”

Section: Introductionmentioning

confidence: 99%

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5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Cortés‐Gutiérrez

Ortíz-Hernández

Dávila-Rodríguez

et al. 2013

IJMS

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We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Cortés‐Gutiérrez

Ortíz-Hernández

Dávila-Rodríguez

et al. 2013

IJMS

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“…A number of studies, using fluorescence in situ hybridization and/or karyotyping, investigated both numerical and structural abnormalities of chromosome 1 in cervical carcinomas and its precursor lesions (45)(46)(47)(48). These studies showed that genomic alterations of chromosome 1 are restricted to high-grade dysplasia and carcinoma of the cervix.…”

Section: Discussionmentioning

confidence: 99%

“…The mean age of women included in this study was 33.3, ranging from 22 till 54 y. Only samples that contained hrHPV DNA, as determined by a general primer GP5+/GP6+-mediated PCRenzyme immunoassay method using a probe cocktail of 14 hrHPV types (types 16,18,31,33,35,39,45,51,52,56,58,59, 66, and 68), were included (23). Presence of CIN1 or CIN2/3 in the frozen specimens used for array CGH experiments was independently validated by two experienced pathologists (FJvK and CJLMM).…”

Section: Methodsmentioning

confidence: 99%

Chromosomal Signatures of a Subset of High-Grade Premalignant Cervical Lesions Closely Resemble Invasive Carcinomas

Wilting¹,

Steenbergen²,

Tijssen³

et al. 2009

Cancer Research

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Cervical cancer develops from precancerous high-grade cervical intraepithelial neoplasia (CIN) harboring a transforming infection with high-risk human papillomavirus, which is characterized by p16INK4a overexpression. Once such a lesion has developed, progression toward an invasive squamous cell carcinoma (SCC) may take one or more decades, underlining the heterogeneity of these lesions in terms of duration of existence and progression risk. We performed array-based comparative genomic hybridization (array CGH) on 46 p16

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“…Besides, it is difficult to analyze some of the complex karyotypes characteristic of many human tumors. On the other hand, FISH is a relatively more convenient and quick method for evaluating tissue cell chromosomal changes [24,25] . Moreover, we have observed that by using adequate fluorescent signal capture (optical sectioning microscopic method) and analysis systems, an accurate estimate of the changes in chromosome copy number could be obtained from tumor tissues, based on which we could make satisfactory interpretations.…”

Section: Discussionmentioning

confidence: 99%

Chromosome 11 aneusomy in esophageal cancers and precancerous lesions- an early event in neoplastic transformation: An interphase fluorescencein situhybridization study from south India

Mohan

Ponnala²,

Reddy³

et al. 2007

WJG

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which may play a role in the neoplastic transformation of esophageal precancerous lesions to cancers. INTRODUCTIONMore than 30% of the adult population exhibits upper gastrointestinal tract disorders associated with symptoms such as regurgitation, heartburn and dysphagia, warranting an endoscopic evaluation. Some of these esophageal pathologies require medication while others can be managed by altering life-style and dietary habits. A certain percentage of these, however, progress into esophageal malignancies. In India, esophageal cancer is the second leading cancer in men and fourth leading cancer in women [1] . Epithelial tumors of the esophagus [squamous cell carcinoma (SCC) and adenocarcinoma (ADC)] are responsible for more than 95% of all esophageal carcinomas. This malignancy presents generally as a locally advanced disease, hence leading to poor prognosis with an average 5-year survival of < 12% in India [2] . Abnormal proliferation of the esophageal epithelial cells with hyperplasia and dysplasia in the normal squamous lining are regarded as premalignant lesions [3,4] . Another common premalignant condition is Barrett's esophagus (BE), where patients have a forty-fold increased risk for developing adenocarcinoma as compared to normal individuals. Although significant advances have been made in the diagnosis and treatment of esophageal carcinomas, not many studies have evaluated markers in the target tissue, in association with increased risk for malignant Abstract AIM: To detect aneusomic changes with respect to chromosome 11 copy number in esophageal precancers and cancers wherein the generation of cancer-specific phenotypes is believed to be associated with specific chromosomal aneuploidies. METHODS:We p e r fo r m e d f l u o r e s c e n c e i n s i t u hybridization (FISH) on esophageal tissue paraffin sections to analyze changes in chromosome 11 copy number using apotome-generated images by optical sectioning microscopy. Sections were prepared from esophageal tumor tissue, tissues showing preneoplastic changes and histologically normal tissues (control) obtained from patients referred to the clinic for endoscopic evaluation. RESULTS:Our results demonstrated that aneusomy was seen in all the cancers and preneoplastic tissues, while none of the controls showed aneusomic cells. There was no increase in aneusomy from precancers to cancers. CONCLUSION:Our results suggest that evaluation of chromosome 11 aneusomy in esophageal tissue using FISH with an appropriate signal capture-analysis system, can be used as an ancillary molecular marker predictive of early neoplastic changes. Future studies can be directed towards the genes on chromosome 11,

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Association between the stages of cervical cancer and chromosome 1 aneusomy

Cited by 15 publications

References 17 publications

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Chromosomal Signatures of a Subset of High-Grade Premalignant Cervical Lesions Closely Resemble Invasive Carcinomas

Chromosome 11 aneusomy in esophageal cancers and precancerous lesions- an early event in neoplastic transformation: An interphase fluorescencein situhybridization study from south India

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