5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Cortés‐Gutiérrez, Elva I.; Ortíz-Hernández, Brenda L.; Dávila-Rodríguez, Martha I.; Cerda‐Flores, Ricardo M.; Fernández, José Luis Fernández; López‐Fernández, Carmen; Gosálvez, Jaime

doi:10.3390/ijms14024135

Cited by 11 publications

(3 citation statements)

References 33 publications

(33 reference statements)

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“…Genome instability is considered as a predisposing factor and one of the primary events in a malignant transformation [8]. Our preliminary results indicate that micronucleus frequency as a marker of genome instability cannot be used as the only predictor for the evaluation of the risk of malignant transformation in uterine cervix.…”

Section: Discussionmentioning

confidence: 85%

Significance of Micronucleus in Cervical Smears

2017

IJSR

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Section: Discussionmentioning

confidence: 85%

Significance of Micronucleus in Cervical Smears

2017

IJSR

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“…Epigenetics alterations such as global DNA hypomethylation of high repetitive DNA sequences is a biological event that signals genomic instability and have been found in a variety of cancers including cervical cancer (Shuangshoti et al, 2007;Ehrlich, 2009). Chromosomal damage and instability were discovered in peripheral blood lymphocytes and cervical cells obtained from patients with Low grade SIL (squamous intraepithelial lesions, LSIL) and high grade SIL (HSIL) when compared to normal cells (Milošević-Djordjević et al, 2011;Cortés-Gutiérrez et al, 2013).…”

Section: Line-1 and Alu Methylation In Hrhpv-associated Precancerous ...mentioning

confidence: 99%

LINE-1 and Alu Methylation in hrHPV-Associated Precancerous Cervical Samples

Chaiwongkot

Buranapraditkun²,

Chujan³

et al. 2022

Asian Pac J Cancer Prev

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Objective: This study was conducted to determine global DNA methylation patterns in cervical cells cytologically identified as atypical squamous cells of unknown significance (ASCUS) with a normal, LSIL, or HSIL histopathological result. Methods: Methylation patterns of long interspersed nuclear elements (LINE-1) and short interspersed element (Alu) sequences were assessed using the combined bisulfite restriction analysis (COBRA) method in cervical samples with cytology-diagnosed cervical lesions. Results: In cervical precancerous lesions with hrHPV positive, the percentage of overall ( m C) and m C m C LINE-1 methylation levels showed a stepwise increase from hrHPV positive normal to HSIL with significant differences (p<0.001). However, both methylation levels were significantly higher in hrHPV negative normal than in hrHPV positive normal (p<0.001). The overall ( m C) Alu methylation in hrHPV positive LSIL and HSIL was lower than in hrHPV positive normal, with a significant difference (p<0.05). Remarkably, the percentage of u C m C and m C u C of LINE-1 and Alu in three different hrHPV positive cervical lesions showed a stepwise decrease from hrHPV positive normal, LSIL and HSIL, respectively. Furthermore, receiver operating characteristic (ROC) curve analyses revealed that the LINE-1 m C and m C m C patterns have high sensitivity and specificity for distinguishing HSIL from normal/LSIL in hrHPV positive cases at the appropriate cutoff levels. Conclusion: We have demonstrated the LINE-1 and Alu methylation data in normal and premalignant cervical epithelia. LINE-1 hypomethylation was found in hrHPV positive normal cells, with lower methylation levels associated with cancer features. In cytologically diagnosed Atypical Squamous Cells of Unknown Significance (ASCUS), the levels of m C and the m C m C pattern could be utilized in concert with hrHPV detection to classify the ASCUS sample prior to colposcopy.

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“…As the number of DNA breaks increases in a target region, more ssDNA is produced, and more probe hybridizes, resulting in a more intense FISH signal, which can be quantified with image analysis systems. 18-20 Moreover, the alkaline treatment may break the sugar-phosphate backbone at abasic sites or at sites with deoxyribose damage, thus transforming these lesions into DNA breaks that are also converted into ssDNA.…”

Section: Introductionmentioning

confidence: 99%

Expression of the HPV18/E6 oncoprotein induces DNA damage

et al. 2017

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This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

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5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Cited by 11 publications

References 33 publications

Significance of Micronucleus in Cervical Smears

Significance of Micronucleus in Cervical Smears

LINE-1 and Alu Methylation in hrHPV-Associated Precancerous Cervical Samples

Expression of the HPV18/E6 oncoprotein induces DNA damage

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