Association between Serum Level of Multiple Trace Elements and Esophageal Squamous Cell Carcinoma Risk: A Case–Control Study in China

Abstract

We investigated the associations between multiple serum trace element levels and risk for esophageal squamous cell carcinoma (ESCC). A total of 185 ESCC patients and 191 healthy individuals were recruited in our study. The concentration of 13 trace elements (Al, V, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Sr, Cd and Pb) in serum was determined with inductively coupled plasma mass spectrometry (ICP-MS). Logistic regression and the Probit extension of Bayesian Kernel Machine Regression (BKMR) models was established to explore the associations and the cumulative and mixed effects of multiple trace elements on ESCC. Three elements (Zn, Se and Sr) displayed a negative trend with risk for ESCC, and a significant overall effect of the mixture of Al, V, Mn, Ni, Zn, Se and Sr on ESCC was found, with the effects of V, Ni and Sr being nonlinear. Bivariate exposure–response interactions among these trace elements indicated a synergistic effect between Zn and Se, and an impactful difference of V combined with Ni, Sr or Zn. Our results indicate that Ni, V, Al, Mn, Zn, Se and Sr are associated with ESCC risk, providing additional evidence of the complex effects of trace elements disorder during the etiology of EC development.

Silver Nanoparticles Cause Neural and Vascular Disruption by Affecting Key Neuroactive Ligand-Receptor Interaction and VEGF Signaling Pathways

“…The metal ion detection in tissues was determined according to a previously described protocol. 19 Briefly, zebrafish embryos treated with different concentrations of AgNP for 72 h (n = 100 for each group) were washed with ultrapure water (Milli-Q, Millipore Inc., Bedford, MA) before storing in the ultra-low temperature freezer for the following experiments. Before digestion, all samples were transferred to a Teflon tube and dried in an oven at 80 °C for 8 h. After that, the sample was digested with 250 uL 65% nitric acid (Merk, Darmstadt, Germany) in a water bath at 80 °C for 1 h, and then diluted with 3% nitric acid, and analyzed by using ICP-MS (Agilent 7900, Agilent Technologies, Santa Clara, CA).…”