Association between Repeated Unpredictable Chronic Mild Stress (UCMS) Procedures with a High Fat Diet: A Model of Fluoxetine Resistance in Mice

Isingrini, Elsa; Camus, Vincent; Guisquet, Anne-Marie Le; Pingaud, M; Devers, Séverine; Belzung, Catherine

doi:10.1371/journal.pone.0010404

Cited by 199 publications

(102 citation statements)

References 42 publications

(61 reference statements)

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“…5A). We then used the cookie test paradigm, another test that evaluates hedonic behavior (29,30). Mice were placed in the first compartment and traveled to reach the cookie in the third compartment (Fig.…”

Section: -Ht1b Receptor In Cholinergic Neurons Regulates Hedonic Behmentioning

confidence: 99%

Opposing roles for serotonin in cholinergic neurons of the ventral and dorsal striatum

Virk

Sagi

Medrihan

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Little is known about the molecular similarities and differences between neurons in the ventral (vSt) and dorsal striatum (dSt) and their physiological implications. In the vSt, serotonin [5-Hydroxytryptamine (5-HT)] modulates mood control and pleasure response, whereas in the dSt, 5-HT regulates motor behavior. Here we show that, in mice, 5-HT depolarizes cholinergic interneurons (ChIs) of the dSt whereas hyperpolarizing ChIs from the vSt by acting on different 5-HT receptor isoforms. In the vSt, 5-HT1A (a postsynaptic receptor) and 5-HT1B (a presynaptic receptor) are highly expressed, and synergistically inhibit the excitability of ChIs. The inhibitory modulation by 5-HT1B, but not that by 5-HT1A, is mediated by p11, a protein associated with major depressive disorder. Specific deletion of 5-HT1B from cholinergic neurons results in impaired inhibition of ACh release in the vSt and in anhedonic-like behavior.5-HT1A | 5-HT1B | cholinergic interneurons | ventral striatum | TRAP C holinergic interneurons (ChIs) represent only 1-2% of all striatal neurons (1). Despite their low abundance, ChIs play a major role in striatal function, by modulating both inputs to and outputs from spiny projection neurons (SPNs) (2). In the ventral striatum (vSt), ChIs are thought to play a major role in mediating reward, motivation, food intake, and hedonic behavior, whereas ChIs of the dorsal striatum (dSt) are implicated in motor behavior and action selection (3-5). Despite their functional differences, only a few morphological differences between vSt and dSt ChIs have been demonstrated, but no molecular differences between these two populations have been reported (6).Serotonin [5-Hydroxytryptamine (5-HT)] signaling in the striatum has long been implicated in modulating locomotion as well as in mood control (7-9). Striatal 5-HT levels are high, and multiple 5-HT receptors (5-HTRs) are thought to mediate the function of 5-HT in these circuits (7, 10). In dSt ChIs, several 5-HTRs (5-HT7, 5-HT6, and 5-HT2) have been reported to induce membrane depolarization (11,12), but the role of 5-HT signaling in vSt ChIs and its implication for mood regulation are poorly understood. To elucidate the role of 5-HT in vSt ChIs, we used electrophysiological recordings, optogenetics, and cell type-specific gene expression analysis. We demonstrate that 5-HT1A and 5-HT1B synergistically inhibit the function of vSt ChIs. The effect of activation of 5-HT1B, but not that of 5-HT1A, is mediated by p11. Furthermore, deletion of 5-HT1B from cholinergic neurons resulted in a loss of pleasure response (anhedonia), a core symptom of major depressive disorder. ResultsOpposite Effects of 5-HT on Cell Excitability Between vSt ChIs and dSt ChIs. To investigate the effect of 5-HT on ChIs from the vSt and dSt, we carried out electrophysiological recordings from visually identified neurons in acute brain slices. To identify ChIs, we used translating ribosome affinity purification (TRAP) mice expressing a GFP-tagged ribosomal protein, L10a, under the choline acetyltrans...

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Section: -Ht1b Receptor In Cholinergic Neurons Regulates Hedonic Behmentioning

confidence: 99%

Opposing roles for serotonin in cholinergic neurons of the ventral and dorsal striatum

Virk

Sagi

Medrihan

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…CS and BW of the animals were recorded on each Monday before and during the experiments. The evaluation of the CS was carried out by assessment of eight different body parts: head (including eyes and nose); neck; dorsal coat;ventral coat; tail; forepaws; hind paws; and, genital region [16,18]. A score of "0" for a coat in a good state or a score of "1" for a dirty coat were given for each of these areas.…”

Section: Behavioural Testsmentioning

confidence: 99%

“…A sucrose solution (10%) was sprayed on the neck and dorsal coat of mice. The total number of grooming was recorded for 5 min after vaporization of the sucrose solution [18]. The observer was unaware of the treatment conditions.…”

Section: Behavioural Testsmentioning

confidence: 99%

Dipyrone ameliorates behavioural changes induced by unpredictable chronic mild stress: gender differences

Kıroğlu¹,

Demirkol²,

Berktaş³

et al. 2016

CIM

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Dipyrone ameliorates behavioural changes induced by unpredictable chronic mild stress: gender differences Abstract Purpose: Antidepressant effects of analgesics have been investigate in both clinical and experimental studies. The purpose of this study was to investigate if the analgesic-antipyretic drug, dipyrone, also had antidepressant-like effects.Methods: Depression-like effects were investigated in an unpredictable chronic mild stress (UCMS) model in both male and female mice. Cage changes, light-dark cycle reversal, cage tilting, wet floor, empty cage, foreign material on the floor and predator sounds were used to induce light stress at different times for six weeks. Dipyrone was administered intraperitoneally beginning from the third week. Splash, rota-rod (RR) and forced swimming (FST) tests were performed at the seventh week as behavioural tests to evaluate the antidepressant-like effects of dipyrone. Coat state score (CSS) and weights of animals were recorded at seventh weeks. Results were analyzed using one or two-way ANOVA followed by the Bonferonni post hoc test.Results: Weight of UCMS-exposed mice did not change compared with controls; however, significant changes were observed in CSS in both sexes of stressed mice (p<0.05). RR latency decreased and immobility time enhanced in FST test in both sexes of stressed mice (p<0.05). Grooming behaviour was not different between the groups in female mice, but different in male mice in the splash test. Dipyrone did not produce a significant change in CSS in the UCMS-exposed group but reversed the latency time and immobility time to normal values in both sexes of mice and augmented the number of grooming behaviour only in stressed male mice. Conclusion:These results indicate that dipyrone produce antidepressant-like effects to some symptoms of UCMS according to gender.SUPPLEMENT

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“…More recently, follow-up work has taken a proteomic approach in an attempt to find molecular differences in the ventral hippocampus between responders and non-responders (Bisgaard et al, 2007). Another study demonstrated that if animals are on a high fat diet during multiple UCMS procedures they become resistant to treatment with a SSRI (fluoxetine) (Isingrini et al, 2010).…”

Section: Non-response/resistance In the Unpredictable Chronic Mild Stmentioning

confidence: 99%

Non-Response to Initial Antidepressant Therapy

Guilloux¹,

David²,

Samuels³

et al. 2012

Psychology - Selected Papers

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Association between Repeated Unpredictable Chronic Mild Stress (UCMS) Procedures with a High Fat Diet: A Model of Fluoxetine Resistance in Mice

Cited by 199 publications

References 42 publications

Opposing roles for serotonin in cholinergic neurons of the ventral and dorsal striatum

Opposing roles for serotonin in cholinergic neurons of the ventral and dorsal striatum

Dipyrone ameliorates behavioural changes induced by unpredictable chronic mild stress: gender differences

Non-Response to Initial Antidepressant Therapy

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