Association between proinflammatory cytokines and lipid peroxidation in patients with severe dengue disease around defervescence

Rajendiran, Soundravally; Hoti, S.L.; Patil, Shripad A.; Cleetus, Cherupanakkal; Bobby, Zachariah; Kadhiravan, Tamilarasu; Parameswaran, Narayanan; Kumar, Brajendra

doi:10.1016/j.ijid.2013.09.022

Cited by 76 publications

(72 citation statements)

References 37 publications

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“…The ROS generated during the innate immune response will induce the redox homeostasis mechanism through the activation of antioxidant defence [17]. We have earlier reported that increased levels of inflammatory cytokines along with lipid peroxides in various clinical spectrum of dengue disease [21]. The present study shows that Nox-2 as well as Nrf-2gene expression was attenuated in all study groups including OFI during DOA.…”

Section: Discussionsupporting

confidence: 57%

Differential expression of NADPH oxidase-2 (Nox-2) and nuclear factor-erythroid 2-related factor 2 (Nrf2) transcripts in peripheral blood mononuclear cells isolated from dengue patients

et al. 2017

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The role of oxidative stress in the pathogenesis of dengue infection is not completely known. A recent study reveals the involvement of oxidative stress responsive molecules in the generation of host immune responses to dengue virus in vitro. Objective of the present study was to analyse the changes in the expression of oxidant-antioxidant genes Nox-2 (NADPH oxidase) and Nrf2 (nuclear factor-erythroid 2-related factor 2) in patients with dengue during the early phase of infection compared to other febrile illness (OFI) cases and healthy controls using Real-time qPCR assay. The study enrolled 88 dengue patients, 31 OFI cases, and 63 healthy individuals as controls. Out of 88 dengue cases, 32 were classified as severe dengue cases (SD) and remaining 56 patients as non-severe dengue (NSD). Blood samples were collected firstly at the time of admission and a second sampling was done from the available individuals (38 dengue and 13 OFI cases) at the time of defervescence. Total RNA was extracted from the Peripheral blood mononuclear cells and the transcripts level of Nox-2 and Nrf2 were analysed by qPCR. On DOA, both Nox-2 and Nrf2 expression was found to be down regulated in dengue and OFI cases (P \ 0.05) compared to healthy controls. Interestingly at defervescence, the transcript levels were found to be significantly increased in dengue cases unlike OFI, where no such increment was evidenced. From DOA to DOD, the study observed a signficant increase in the levels of Nox-2 transcripts (P \ 0.05) both in SD and NSD cases. But a significant Nrf2 activation was not observed in SD cases as we found in NSD cases. Thus a steady and significant increase in Nox-2 transcript level in severe, non-severe and secondary dengue infected groups observed in the current study supports the earlier reports on the involvement of anti-oxidant response in dengue severity. However further studies on its protein levels and mechanistic action would decipher the exact role of these potential molecules in the disease virulence.

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Section: Discussionsupporting

confidence: 57%

Differential expression of NADPH oxidase-2 (Nox-2) and nuclear factor-erythroid 2-related factor 2 (Nrf2) transcripts in peripheral blood mononuclear cells isolated from dengue patients

et al. 2017

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“…Ten studies noted affirmatively that their first blood sampling was performed at the febrile phase, which we defined here as <5 days or <96 h from fever onset (Brasier et al, 2012;Butthep et al, 2012;Green et al, 1999a, b;De La Cruz Hern andez et al, 2014;Malavige et al, 2013a;P erez et al, 2004;Soundravally et al, 2014;Wang et al, 2007). The remaining studies had wider blood sampling windows, ranging from 1 to 10 days from symptom onset (Arias et al, 2014;Bozza et al, 2008;Chen et al, 2005;de-Oliveira-Pinto et al, 2012;Guerrero et al, 2013;Houghton-Triviño et al, 2010;Kurane et al, 1991Kurane et al, , 1993Laur et al, 1998;Levy et al, 2010;Del Moral-Hern andez et al, 2014;Nguyen et al, 2004;Suharti et al, 2003).…”

Section: Study Characteristicsmentioning

confidence: 97%

“…Studies differed in the types of immuno-modulating proteins investigated, and the most frequently profiled cytokines and chemokines were TNFa (12/20, 60 %), IFNg (11/20, 55 %), IL-6 (10/20, 50 %), IL-10 (9/20, 45 %), IL-4 (6/20, 30 %) and IFNa (3/20, 15 %). Despite being the most investigated cytokine, TNFa was significantly elevated in DHF patients in only five studies (Houghton-Triviño et al, 2010;Levy et al, 2010;Nguyen et al, 2004;Soundravally et al, 2014;Wang et al, 2007), and one study found no statistical difference in TNFa levels between DF and DHF patients (Laur et al, 1998). The rest of studies reported no statistical difference or did not detect TNFa.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…A total of 19 out of 24 studies were case-control studies (Arias et al, 2014;Bozza et al, 2008;Brasier et al, 2012;Butthep et al, 2012;Chen et al, 2005;Furuta et al, 2012;Green et al, 1999a, b;Guerrero et al, 2013;Houghton-Triviño et al, 2010;De La Cruz Hern andez et al, 2014;Laur et al, 1998;Levy et al, 2010;Malavige et al, 2013a;Nguyen et al, 2004;Del Moral-Hern andez et al, 2014;P erez et al, 2004;Soundravally et al, 2014;Wang et al, 2007), and the remaining 5 studies were prospective cohort studies (de-Oliveira-Pinto et al, 2012;Kurane et al, 1991Kurane et al, , 1993Suharti et al, 2003). Comparison groups were healthy controls, patients with other febrile illness (OFI) or non-severe disease patients (DF or non-SD); however, comparisons made between SD and non-SD cases were considered most ideal for the purpose of this review.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…By contrast, IL-10 stood out as the cytokine for which concentration was elevated in DHF relative to DF patients consistently in seven studies utilizing the WHO (1986)/WHO (1997) case definition (Table 3) (Brasier et al, 2012;Butthep et al, 2012;Chen et al, 2005;Green et al, 1999b;Houghton-Triviño et al, 2010;Nguyen et al, 2004;P erez et al, 2004). The association of dengue severity with circulating levels of IFNg was more heterogeneous (Table 4) (Bozza et al, 2008;Butthep et al, 2012;Chen et al, 2005;Nguyen et al, 2004;Soundravally et al, 2014). Three studies noted increased IFNg in DHF and/or DSS patients relative to DF patients or healthy controls (Bozza et al, 2008;Butthep et al, 2012;Nguyen et al, 2004), whereas three studies noted decreased IFNg levels in DHF/DSS over DF patients (Chen et al, 2005;Soundravally et al, 2014).…”

Section: Study Characteristicsmentioning

confidence: 99%

See 2 more Smart Citations

Markers of dengue severity: a systematic review of cytokines and chemokines

Lee

Leong

Wilder‐Smith

2016

Journal of General Virology

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The prognosis of dengue remains a challenge in the early, objective triage of patients with dengue fever of differing severity. Circulating immuno-modulating proteins have brought new possibilities as prognostic markers of severe dengue (SD). This systematic review is devoted to understanding the potential utility of blood-based cytokines and chemokines as prognostication markers of SD based on the current literature. PubMed and Embase were searched. Of 794 candidate articles, 685 abstracts were screened against our exclusion/inclusion criteria and 25 (3.6 %) studies met the quality assessments. A total of 18 studies were retrospective observational and 2 were prospective cohort studies. Elevated IL-10, up to day 7 of fever onset, stood out as a candidate prognostic marker for SD using the 1997 and 2009 World Health Organization (WHO) case definitions. IFNg was another potential prognostic marker of SD (1997 WHO case definition), but its levels varied between studies. Significant heterogeneity in methodologies and patient cohorts prevent ready application of IL-10 and IFNg as prognostic markers to other dengue populations. Our results suggest that the current non-randomized studies are delivering inconsistent messages and higher-quality studies, with consistent methodologies and validation in independent patient cohorts, are needed to delineate confounding variables. Major gaps identified were full accounting and transparency of sampling days, dengue virus type, infection status and age group.

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Redox control of vascular biology

et al. 2019

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Redox control is lost when the antioxidant defense system cannot remove abnormally high concentrations of signaling molecules, such as reactive oxygen species (ROS). Chronically elevated levels of ROS cause oxidative stress that may eventually lead to cancer and cardiovascular and neurodegenerative diseases. In this review, we focus on redox effects in the vascular system. We pay close attention to the subcompartments of the vascular system (endothelium, smooth muscle cell layer) and give an overview of how redox changes influence those different compartments. We also review the core aspects of redox biology, cardiovascular physiology, and pathophysiology. Moreover, the topic‐specific knowledgebase DES‐RedoxVasc was used to develop two case studies, one focused on endothelial cells and the other on the vascular smooth muscle cells, as a starting point to possibly extend our knowledge of redox control in vascular biology.

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Association between proinflammatory cytokines and lipid peroxidation in patients with severe dengue disease around defervescence

Cited by 76 publications

References 37 publications

Differential expression of NADPH oxidase-2 (Nox-2) and nuclear factor-erythroid 2-related factor 2 (Nrf2) transcripts in peripheral blood mononuclear cells isolated from dengue patients

Differential expression of NADPH oxidase-2 (Nox-2) and nuclear factor-erythroid 2-related factor 2 (Nrf2) transcripts in peripheral blood mononuclear cells isolated from dengue patients

Markers of dengue severity: a systematic review of cytokines and chemokines

Redox control of vascular biology

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