Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses

Abstract

BackgroundIndependent studies have shown that several single nucleotide polymorphisms (SNP) in the human FTO (fat mass and obesity associated) gene are associated with obesity. SNP have also been identified in the pig FTO gene, among which some are associated with selected fat-deposition traits in F2 crosses and commercial populations. In this study, using both commercial pig populations and an experimental Meishan × Pietrain F2 population, we have investigated the association between one FTO SNP and several growth and carcass traits. Association analyses were performed with the FTO polymorphism either alone or in combination with polymorphisms in flanking loci.MethodsSNP (FM244720:g.400C>G) in exon 3 of porcine FTO was genotyped by PCR-RFLP and tested for associations with some growth, carcass and fat-related traits. Proportions of genetic variance of four pig chromosome 6 genes (FTO, RYR1, LIPE and TGFB1) on selected traits were evaluated using single- and multi-locus models.ResultsLinkage analysis placed FTO on the p arm of pig chromosome 6, approximately 22 cM from RYR1. In the commercial populations, allele C of the FTO SNP was significantly associated with back fat depth and allele G with muscling traits. In the Meishan × Pietrain F2 pigs, heterozygotes with allele C from the Pietrain sows and allele G from the Meishan boar were more significantly associated with fat-related traits compared to homozygotes with allele G from the Pietrain and allele G from the Meishan breed. In single- and multi-locus models, genes RYR1, TGFB1 and FTO showed high associations. The contribution in genetic variance from the polymorphism in the FTO gene was highest for back fat depth, meat area on the musculus longissimus lumborum et thoracis tissues and metabolite glucose-6-phosphate dehydrogenase.ConclusionsOur results show that in pig, FTO influences back fat depth in the commercial populations, while in the Meishan × Pietrain F2 pigs with a CG genotype, heterosis occurs for several fat-related traits.

Association between FTO polymorphism in exon 3 with carcass and meat quality traits in crossbred ducks

“…In commercial pig populations, allele C of the FTO SNP g.400C>G in exon 3 is significantly associated with backfat depth, and allele G is significantly associated with muscle traits and has the greatest effect on thoracic tissues (Dvořáková et al, 2012). The FTO SNPs c.499G>A and c.453C>A in exon 3 are significantly associated with BW at 35, 70, and 84 days of age in New Zealand rabbits (P < 0.01), and the synonymous SNP c.660T>C is significantly associated with BW at 84 days of age, ADG, and the IMF content of the longissimus lumborum in Ira rabbits (P < 0.01) (Zhang et al, 2013).…”

Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles

“…CZL and CZLW were used for crossing in seven of the populations; one population was a purebred CZLW. The populations were maintained in air-conditioned stables under conditions described by Dvořáková et al (2012). The pigs were slaughtered at a target weight of 115 kg (SD ± 12.7).…”

“…Carcass dissection was performed using the method described by Walstra and Merkus (1995), and standard carcass traits were measured. The following performance traits were also recorded: live weight at slaughter, feed conversion ratio, and average daily gain; back fat depth (see Dvořáková et al, 2012); intramuscular fat in the neck, shoulder, and m.l.l.t., and fat content of the belly (determined by the Soxhlet method, which uses gravimetric determination in accordance with the Czech Standard, ČSN ISO 1443(1973; for information on the belly see Stupka et al, 2004); fat and muscle depth and lean meat percentage (measured with Fat-O-Meter (SFK, Viborg, Denmark) (Šprysl et al, 2007) and TPtwo-point method (Krška et al, 2002) For practical and organizational reasons, individual performance traits were not recorded for all of the animals; therefore, different numbers of pigs were analyzed per trait. All pigs were slaughtered according to the protocols for certified Czech slaughterhouses under the supervision of an independent veterinarian.…”

Histological characteristics of the musculus longissimus lumborum et thoracis muscle fibres in pigs in relation to selected RYR1, MYOG, MYOD1 and MYF6 genotypes

“…Fujii et al (1991) inform about a single-point RYR1 gene mutation g.1843C> T, which is related to malignant hyperthermia in pigs. Changes in the RYR1 gene influence the cell membrane of muscle cells and lead to an increased release of the Ca 2+ ions from the sarcoplasmic reticulum, as a response to stress stimuli (Dvořáková et al 2012). The MYOD1 gene was located on the 2 nd chromosome (Soumillion et al 1997), in the 2p1.4-1.7 area.…”