Association Between PCR-SSCP of Bone Morphogenetic Protein 15 Gene and Prolificacy in Jining Grey Goats

Chu, Mingxing; Jiao, Chengzhi; He, Yi; Wang, J. Y.; Liu, Z. H.; Chen, G. H.

doi:10.1080/10495390701331114

Cited by 35 publications

(22 citation statements)

References 40 publications

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“…FecX I mutation was also not found in Chinese Merino (54), Xinjiang Fine Wool (35) and Romolly Hills (40; Dai 2004 Although there are many reports on mutations in a single gene of fecundity gene family, mutations at the multiple loci of fecundity genes have been reported in three sheep breeds only. These include the Belclare/ Cambridge sheep with mutations at GDF9 and BMP15 loci (Hanrahan et al 2004), the Lacaune sheep at FecL and BMP15 loci (Bodin et al 2002) and Small-tailed Han sheep at BMPRIB and BMP15 loci (Chu et al 2007). To the best knowledge of the authors, the Garole sheep is therefore the fourth sheep breed that has coexisting mutations in two different fecundity genes (BMPR1B and GDF9).…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep

Polley

Brahma

et al. 2009

Trop Anim Health Prod

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Mutation studies in different prolific sheep breeds have shown that the transforming growth factor beta super family ligands viz. the growth differentiation factor 9 (GDF9/FecG), bone morphogenetic protein 15 (BMP15/FecX) and associated type I receptors, bone morphogenetic protein receptor (BMPR1B/FecB), are major determinant of ovulation rate and consequent increase in litter size. The Garole sheep is a highly prolific sheep breed of India. Characterization of fecundity genes in these animals could substantially improvise the breeding programme in these animals as well as other sheep breeds of the region. The present study was therefore designed with the objective of polymorphism study of fecundity genes in these prolific microsheep. A total of 11 point mutations were detected by polymerase chain reaction (PCR)-based method. A competitive technique called tetra-primer amplification refractory mutation system-PCR was adapted to type a total of ten points of two ovine fecundity genes (GDF9 and BMP15). The FecB locus of the BMPR1B gene and G1 locus of GDF9 gene were found to be polymorphic. In FecB locus, two genotypes, wild type (FecB(+)) and mutant (FecBB), were detected with allele frequencies of 0.39 and 0.61, respectively. At G1 locus, two genotypes, mutant (A) and wild types (G) were detected with allele frequencies of 0.18 and 0.82, respectively. This study reports Garole sheep as the fourth sheep breed after Belclare/Cambridge, Lacaune and Small-tailed Han sheep, where coexisting polymorphism has been found in two different fecundity genes (BMPRIB and GDF9 genes).

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Section: Discussionmentioning

confidence: 99%

Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep

Polley

Brahma

et al. 2009

Trop Anim Health Prod

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“…P values <0.05 were significant. The statistical analysis model of litter size in Jining Grey goats was the same as Chu et al (10), in which l is the population mean, S is the fixed sire effect (S ¼ 1, 2, 3, 4, 5), KS is the fixed effect of kidding season (KS ¼ 1, 2, 3, 4), P is the fixed parity effect (P ¼ 1, 2, 3), G is the fixed effect for GPR54 genotype (G ¼ 1, 2, 3), and e is the random residual effect of each observation.…”

Section: Methodsmentioning

confidence: 99%

Association Between Sexual Precocity and Alleles ofKISS-1andGPR54Genes in Goats

Feng

Zhao

Chu

et al. 2009

Animal Biotechnology

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KISS-1 and GPR54 were regarded as key regulators for the puberty onset and fundamental gatekeepers of sexual maturation in mammals. To explore the possible association between variations in KISS-1 and GPR54 with sexual precocity, mutation screening of exon 1 of KISS-1 and exon 1, exon 3, and partial exon 5 of GPR54 was performed in a sexual precocious breed (Jining Grey goats) and sexual late-maturing breeds (Inner Mongolia Cashmere, Angora, and Boer goats) by PCR-SSCP. The results showed that five novel mutations were identified in exon 1 and partial exon 5 of GPR54 including C96 T, T173C, G176A, G825A, and C981 T. The Jining Grey goats with genotype BB or AB had 1.07 (P < 0.05) or 0.40 (P < 0.05) kids more than those with AA. The Jining Grey goats with genotype DD or CD had 1.80 (P < 0.05) or 0.55 (P < 0.05) kids more than CC, respectively. The present study preliminarily showed an association between alleles B and D of GPR54 with high litter size and sexual precocity in Jining Grey goats.

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“…Mutations of A898G (also named as A963G and resulted in S300G in protein) and C985G (also named as C1050G and resulted in L329V in protein) were reported in exon 2 of the BMP15 gene in Jining Grey goats (14,17). Another 2 novel SNPs (G735A and C808G) were observed in exon 2 of the BMP15 gene, which showed no evidence of being related to litter size in Indian goat breeds (23 , and FecX R , were associated with both increased ovulation rate or litter size in heterozygous carriers and sterility in homozygous carriers, which had no significant effect on fecundity of JG goats (13)(14)(15)(16).…”

Section: Resultsmentioning

confidence: 99%